In vitro organogenesis in some citrus species

Abstract

ABSTRACT-In vitro organogenesis of citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP -0.0; 0.5; 1.0; 1.5, or 2.0 mg L -1 ). For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L -1 of BAP. The efficiency of culture medium supplementation with the combination of BAP (0.0; 1.0, or 2.0 mg L -1 ) and NAA (1-naphthaleneacetic acid -0.0; 0.3, or 0.5 mg L -1 ) in the development of adventitious shoots was evaluated for C. aurantium. Culture medium supplementation with BAP is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. In general, culture media supplementation with BAP decreased the percentage of responsive explants excepted for C. sinensis cv. 'Natal' and C. limonia when the concentrations of 1.5 and 2.0 mg/L -1 were used. The presence of cytokinin, in concentrations up to 2 mg/L -1 , stimulated the in vitro organogenesis when internodal segments-derived explants were used for C. limonia and C. aurantium. For C. aurantium no adventitious shoots developed in explants (internodal segments) cultured in basal culture medium, without BAP supplementation. Although no statistic differences could be detected, culture media supplementation with the combination of BAP and NAA favored the development of adventitious shoots in C. aurantium. The best concentration of NAA varied according to BAP concentration. The results presented herein show that citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype. Index terms: epicotyl segment, internodal segment, C. sinensis, C. limonia, C. volkameriana, C. aurantium. ORGANOGÊNESE IN VITRO EM ALGUMAS ESPÉCIES DE CITRUSRESUMO-A organogênese in vitro de citrus foi estudada para os genótipos Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana e C. aurantium, utilizando-se de segmentos de epicótilo como explante, cultivados em meio de cultura MT suplementado com diferentes concentrações de 6-benzilaminopurina (BAP -0,0; 0,5; 1,0; 1,5 ou 2,0 mg L -1 ). Para os genótipos recalcitrantes C. limonia e C. aurantium, a organogênese in vitro também foi estudada, utilizando-se de segmento internodal como explante, cultivado em meio de cultura MT suplementado com 0; 0,5; 1,0; 2,0 ou 4,0 mg L -1 de BAP. A eficiência da suplementação do meio de cultura com a combinação de BAP (0,0; 1,0 ou 2,0 mg L -1 ) e NAA (0,0; 0,3 ou 0,5 mg L -1 ) no desenvolvimento de gemas adventícias foi avaliada para C. aurantium. Pode-se verificar que, nos quatro genótipos estudados, a suplementação do meio de cultura com BAP não é essencial para o desenvolviment...

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

“…The highest number of shoots were obtained in 1.5 mg/l BAP, 0.5 mg/l BAP + 1 mg/l Kn and 0.5 mg/l Kn + 1 mg/l BAP + 1 mg/l 2,4-D. Similar result was reported in Citrus volkameriana (Schinor et al 2011) where the number of shoots increased when 1.5 mg/l BAP was used. The multiplication of shoots appeared to be affected by the use of BAP.…”

Effect of Spraying with Coconut Liquid and Yeast Extract on Vegetative Growth Traits of Citrus rootstock (Citrus aurantium and Volkameriana)

“…The results of the table showed the significant effect of the triple interference of the experimental factors in the characteristic of plant height, as the interference treatment the rootstock of Volkameriana and coconut liquid 200 g L -1 and yeast extract at a concentration of 8 g L -1 achieved the highest rate of 37.863 g compared to the interference treatment of the rootstock of Citrus aurantium and coconut liquid 0 g L -1 and yeast extract 0 g L -1 as it recorded the lowest rate of 17.903g an increase of 111.489% . Tables (1,2,3,4,5,6) show the superiority of the Volkameriana rootstock over the Citrus aurantium rootstock in the studied vegetative traits, which may be due to the nature of the genetic structure of the root and may be due to the high specifications that characterize this rootstock and its positive effect on the grafts growing on it and its compatibility with most types of citrus fruits as well as its resistance to rapid deterioration disease, wood pecking, and viroid bark scaling disease [9] and [20]. Also, this root is considered the best in terms of characteristics compared to Citrus aurantium and other rootstocks, and this is consistent with what was confirmed by [21] and [22], where they confirmed that it is the best in terms of vegetative growth characteristics compared to the rootstock of Citrus aurantium.…”

Determination of the best culture medium for establishment, propagation and conservation in vitro of citrus rootstocks

“…The variations in the regenerative responses observed for the different rootstocks can be attributed to their particular genetic traits, so that genotype has a strong influence on the success of in vitro culture (SCHINOR et al, 2011).…”