In vitro organogenesis and efficient plant regeneration from root explants of Passiflora suberosa L. (Passifloraceae)

In Vitro Organogenesis from Root Explants of Passiflora miniata Mast., an Amazonian Species with Ornamental Potential

“…Successful regeneration protocols based on the use of the root as explant source has been described for several plant species [25][26][27]. In Passiflora, a protocol using root explants for shoot organogenesis was stablished for P. cinicinnata [28,14], P. edulis [14,15,29], P. setacea [11] and P. suberosa [16]. Organogenesis is a regeneration system based in the formation of unipolar adventitious organs with vascular connection to the original explant [30,31,32].…”

“…Unlike, most of the adventitious shoots were formed directly (52%) in TDZ-treatments, as observed in Figure 2. For most protocols, MS medium supplemented with BA concentrations range from 2.2 to 8.8 µM has been used for shoot bud induction from root explants of passion fruit species [14,11,16]. However, few of those studies have tested TDZ [11]).…”

“…leaf, hypocotyl, root, nodal and intermodal segments and zygotic embryos [5][6][7][8][9][10][11][12][13]. At present, root segments have been adopted as explant source for in vitro regeneration of passion fruit [14,15,11,16]; for being easily obtained and maintained under in vitro conditions, in general [17]. In addition, they have shown high regenerative potential in shoot production when compared with other non-meristematic Passiflora explants [18,14].…”

A simple and fast Agrobacterium-mediated transformation system for hybrid passion fruit (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa)

“…BAP concentrations tested had the potential to successfully induce shoots, nevertheless, the induction results indicated that 2.0 mg L −1 BAP was the optimum concentration with a higher efficiency on shoots induction compared to the other concentrations. BAP has been reported as an efficient cytokinin that induces regeneration of shoots in many plants including passion fruit species such as P. edulis f. flavicarpa, P. caerulea, P. alata and P. setacea [19][20][21]. However, on medium containing 2.0 mg L −1 BAP, the induced shoots of KPF4 did not proliferate or elongate and the problem became severe after six weeks of culture.…”

Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulisSims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

“…One of the most appropriate methods of clonal propagation is the use of tissue-culture technology for the production of high-quality and disease-free planting material in a short span of time. ere are many reports on in vitro regeneration of different Passiflora species using different types of explants such as root segments, nodes, internodes, and leaf discs [12][13][14]. At present, there is no report on in vitro regeneration of KPF 4 variety cultivated in Kenya and this hinders genetic improvement of the fruit through biotechnological techniques.…”