In vitro optimization of the Gallus domesticus oviduct epithelial cells culture

Kasperczyk, K.; Bajek, Anna; Joachimiak, R.; Walasik, K.; Marszałek, Andrzej; Drewa, Tomasz; Bednarczyk, Marek

doi:10.1016/j.theriogenology.2011.12.029

Cited by 22 publications

(25 citation statements)

References 24 publications

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“…This system allows us to study infections of differentiated epithelial cells under controlled in vitro conditions. Isolation and cultivation of chicken oviduct epithelial cells has been described by others [13,14], and also the preparation of oviduct explants -displaying the epithelial cells in their original settings as in vivo-has been shown to be a useful method to analyse IBV infections [15][16][17]. Our system has the advantage that it displays the immature organ, which differs in the epithelial composition from the mature organ [18].…”

Section: Discussionmentioning

confidence: 94%

Differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus IBV strains QX and B1648 are not related to the sialic acid binding properties of their spike proteins

Mork

Hesse

Rahman

et al. 2014

Vet Res

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The avian coronavirus (AvCoV) infectious bronchitis virus (IBV) is a major poultry pathogen. A characteristic feature of IBV is the occurrence of many different strains belonging to different serotypes, which makes a complete control of the disease by vaccinations a challenging task. Reasons for differences in the tissue tropism and pathogenicity between IBV strains, e.g. a predilection for the kidneys or the oviduct are still an open question. Strains of the QX genotype have been major pathogens in poultry flocks in Asia, Europe and other parts of the world. They are the cause of severe problems with kidney disease and reproductive tract disorders. We analysed infectivity and binding properties of the QX strain and compared them with those of the nephropathogenic strain B1648. As most IBV strains do not infect permanent cell lines and show infection only in primary chicken cells of the target organs, we developed a culture system for chicken oviduct explants. The epithelial cells of the oviduct showed a high susceptibility to infection by the QX strain and were almost resistant to infection by the nephropathogenic B1648 strain. Binding tests with isolated primary oviduct epithelial cells and soluble S1 proteins revealed that S1 proteins of two IBV strains bound with the same efficiency to oviduct epithelial cells. This attachment was sialic acid dependent, indicating that the sugar binding property of IBV spike proteins is not the limiting factor for differences in infection efficiency for the oviduct of the corresponding viruses.

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Section: Discussionmentioning

confidence: 94%

Differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus IBV strains QX and B1648 are not related to the sialic acid binding properties of their spike proteins

Mork

Hesse

Rahman

et al. 2014

Vet Res

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“…POECs were isolated based on methods described in Jung et al (2011) and Kasperczyk et al (2012), with some modifications. Oviduct tissue was collected from a 5-month-old laying-period pigeon that had not been stimulated with hormones.…”

Section: Cell Culturementioning

confidence: 99%

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Zhang¹,

Chen²,

Li³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs on the secretion of ovalbumin (OVA) in pigeon oviduct epithelial cells (POECs), we determined the complete coding sequences encoding ER alpha (ERα) and ER beta (ERβ) in pigeons. The abundance of pigeon ERα and ERβ mRNA was detected using quantitative polymerase chain reaction. These results revealed that pigeon ERα is highly expressed in the oviduct, while pigeon ERb is highly expressed in the ovary and kidney. We hypothesize that ERα mRNA predominates over that of ERβ in the oviduct. The expression of ERα can be down- 1927©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (1): 1926-1937 (2014 Characterization and regulation of pigeon ERs regulated by 17β-estradiol, and the knockdown of ERα promoted OVA mRNA expression in cultured POECs, indicating that ERα may play an important role in OVA secretion.

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“…To establish a primary culture system for OMECs, the method for oviduct cell dissociation was slightly modified from that of Jung's and Kasperczyk's studies . Briefly, the oviducts were selected from 16‐week‐old Lohman layers.…”

Section: Methodsmentioning

confidence: 99%

“…To establish a primary culture system for OMECs, the method for oviduct cell dissociation was slightly modified from that of Jung's and Kasperczyk's studies. 21,22 Briefly, the oviducts were selected from 16-week-old Lohman layers. The magnum segments of oviducts were trimmed free of blood and connective tissue, then the magnum was dissected into a 1 mm 3 tissue sample by a sterile ophthalmic scissor, and washed in 37 C preheated PBS (Hycolne, Logan, UT, USA) until liquid clarification.…”

Section: Isolation and Culture For Omecsmentioning

confidence: 99%

Involvement of P38 and ERK1/2 in mitochondrial pathways independent cell apoptosis in oviduct magnum epithelial cells of layers challenged with vanadium

Wang

Huang

Zhang

et al. 2018

Environmental Toxicology

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Vanadium (V) can induce cell apoptosis in layers' oviduct resulting in egg quality reduction. In this study, we investigated the relationship between the mitogen‐activated protein kinase (MAPK)‐signaling pathway and V‐induced apoptosis in poultry oviduct magnum epithelial cells (OMECs). Cultured OMECs were divided into 8 treatment groups: 0 μmol/L V (control), 100 μmol/L V (V100), V100 + P38MAPK inhibitor (SB203580), SB203580, V100 + extracellular signal‐regulated kinases 1 and 2 (ERK1/2) inhibitor (U0126), U0126, V100 + c‐JUN NH2‐terminal kinase (JNK) inhibitor (SP600125), and SP600125. The OMECs were pretreated with the MAPK inhibitors before their treatment with V100 for 12 h. V100 increased the apoptosis of OMECs (P < .05), while 3 MAPK inhibitors suppressed V100‐induced apoptosis P < .05); V100 enhanced the depolarization of △ψm (P < .05), and SB203580 and U0126 alleviated the V100‐induced △ψm decrease (P < .05); V100 downregulated B‐cell lymphoma‐2 (Bcl‐2) and poly [Adenosine diphosphate ribose] polymerase 1 (PARP1) mRNA expression (P < .05), meanwhile it upregulated Bcl‐2 associated x (Bax), Apaf1, cytochrome C (CytC) and cysteine aspartase (caspase) 3, 8, 9 mRNA expression (P < .05). All MAPKs inhibitors alleviated the up‐regulation of V100 for Bax and caspase 3 mRNA expression and down‐regulation of V100 for Bcl‐2 expression (P < .05). SB203580 and U0126 upregulated CytC expression treated by V100 (P < .05), except SP600125, while SB203580 administration resulted in a similar upregulation of PARP1 expression (P < .05). SP600125 can alleviated V triggered p‐P38MAPK (phosphor‐P38), p‐ERK1/2 (phosphor‐ERK1/2), p‐JNK (phosphor‐JNK) increase on OME cells, and SB203580 and U0126 had a similar response to phosphor‐P38 and p‐JNK (P < .05). It concluded that V‐induced apoptosis in OMECs through the activation of P38 and ERK1/2, and by increasing the ratio of Bax/Bcl‐2, which resulted in △ψm decrease, CytC release into the cytosol; consequently caspase 3 is recruited and activated, PARP1 is cleaved, eventually leading to apoptosis.

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In vitro optimization of the Gallus domesticus oviduct epithelial cells culture

Cited by 22 publications

References 24 publications

Differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus IBV strains QX and B1648 are not related to the sialic acid binding properties of their spike proteins

Differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus IBV strains QX and B1648 are not related to the sialic acid binding properties of their spike proteins

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Involvement of P38 and ERK1/2 in mitochondrial pathways independent cell apoptosis in oviduct magnum epithelial cells of layers challenged with vanadium

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