In Vitro Morphogenesis of Some Himalayan Flora Using TDZ: A Potential Plant Growth Regulator

Dhiman, Nisha; Gautam, Nitu; Sareen, Bhuvnesh; Kumari, Pooja; Rajouria, Sapna; Bhattacharya, Amita

doi:10.1007/978-981-10-8004-3_12

Cited by 5 publications

(3 citation statements)

References 71 publications

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“…Thus, this result opens perspectives for the use of TDZ as an inducer of organogenesis and/or somatic embryogenesis in A. urundeuva. The morphogenic potential of TDZ has already been proven for different forest species (Dhiman et al, 2018). It is emphasized that, in the treatments in which ANA was associated with BAP, there was no heating of the shoots (Figure 6C).…”

Section: Multiplication In Vitromentioning

confidence: 61%

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

Silva

Oliveira

Nogueira

et al. 2023

Braz. J. Prod. Eng.

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Aroeira-do-sertão (Astronium urundeuva) apresenta aplicabilidade para fins terapêuticos devido às suas características farmacológicas e para uso madeireiro em razão da sua resistência e com grande durabilidade. Isso acarretou a intensa exploração da espécie, levando-a a ser incluída na lista de espécies ameaçadas de extinção. Em decorrência desta situação, a micropropagação é tida como ferramenta em potencial para a propagação e conservação in vitro da espécie. Diante disso, o objetivo deste trabalho foi avaliar a germinação e o efeito de diferentes concentrações de ANA associada com TDZ e BAP na multiplicação in vitro de A. urundeuva. Segmentos nodais, obtidos a partir de plântulas germinadas in vitro, foram utilizados como explantes. Os mesmos foram inoculados no meio de cultura MS50%, suplementado com as seguintes concentrações de ANA (0,0; 0,025 e 0,050 mg.L-1) associadas com TDZ (0,0; 0,25; 0,50; 0,75 e 1,00 mg.L-1) e BAP (0,0; 0,25; 0,50; 0,75 e 1,00 mg.L-1). Transcorridos 31 dias, foi avaliado o vigor dos explantes, calejamento e número de brotações. O percentual de germinação in vitro das sementes de A. urundeuva foi de 61%, demostrando a viabilidade da técnica para iniciar a micropropagação. O maior vigor dos explantes foi obtido no meio de cultura suplementado somente com BAP, por sua vez, o TDZ induziu distúrbios fisiológicos nos explantes, como intenso calejamento e posterior necrose dos tecidos. Os resultados obtidos indicam a necessidade de maiores estudos em razão do balanço hormonal auxina/citocinas não foi favorável para a multiplicação in vitro da espécie.

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Section: Multiplication In Vitromentioning

confidence: 61%

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

Silva

Oliveira

Nogueira

et al. 2023

Braz. J. Prod. Eng.

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“…Its morpho-regulatory potential has led to its application in plant tissue culture for morphogenic system development. 28 TDZ can be used as an effective bioregulator. It also protects chlorophyll degradation, which can lead to better growth and development of plant tissue in vitro.…”

Section: Applications Of Thidiazuron In Plant Tissue Culturementioning

confidence: 99%

“…obtained a higher yield of fruits per plant and effective fruit mass by the use of TDZ. Its morpho‐regulatory potential has led to its application in plant tissue culture for morphogenic system development 28 . TDZ can be used as an effective bioregulator.…”

Section: Applications Of Thidiazuron In Plant Tissue Culturementioning

confidence: 99%

The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro

Ali

Khan

et al. 2022

Biotech and App Biochem

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Thidiazuron (TDZ) is an active substituted phenyl urea compound that has found a significant role as a plant growth regulator. The most exciting aspect of its function is that it can mimic auxins and cytokinin but is chemically different from these two. Many theories have been put forward, and experiments performed to understand the mode of action of TDZ in callogenesis. One suggested mechanism presents that it works by inhibiting the cytokinin degrading enzymes that compete with cytokinin for an active site on the enzyme. An example is the TDZ‐induced suppressed expression of gibberellic acid (GA) biosynthesis genes encoding GA3 and GA20 oxidases. This is entailed with a slightly increased expression of GA catabolism genes encoding GA20 oxidase. Similarly, one of the recommendations is that TDZ induces the expression of specific genes and transcription regulatory sequences that are either responsible directly for callus formation or in turn induce other auxins or cytokinin for callogenesis. There is no concise review available that discusses the details of TDZ‐induced callus, specifically and other in vitro cultures in general. This review is an attempt to explore all these pathways and mechanisms involved in callogenesis in plants stimulated by TDZ.

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Spore culture, proliferation and plant regeneration of Platycerium Wallichii Hook via multiple pathways

Xiong,

Zeng,

Chen

et al. 2024

Plant Growth Regul

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In Vitro Morphogenesis of Some Himalayan Flora Using TDZ: A Potential Plant Growth Regulator

Cited by 5 publications

References 71 publications

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro

Spore culture, proliferation and plant regeneration of Platycerium Wallichii Hook via multiple pathways

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