In Vitro Models of Vasculogenesis and Angiogenesis

Vailhé, Bruno; Vittet, Daniel; Feige, Jean‐Jacques

doi:10.1038/labinvest.3780252

Cited by 313 publications

(223 citation statements)

References 139 publications

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“…We have exploited the versatility of μVNs to investigate the angiogenic response of an endothelium with and without local interactions with perivascular cells. To begin, we applied vasculogenic medium exogenously through μVNs with perfusion to mimic the proangiogenic environment found in the vicinity of ischemic tissue or solid tumors (24,27). After a week of exposure, μVNs presented the following properties ( Fig.…”

Section: Resultsmentioning

confidence: 99%

In vitro microvessels for the study of angiogenesis and thrombosis

Zheng

Chen

Craven

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

781

842

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Microvascular networks support metabolic activity and define microenvironmental conditions within tissues in health and pathology. Recapitulation of functional microvascular structures in vitro could provide a platform for the study of complex vascular phenomena, including angiogenesis and thrombosis. We have engineered living microvascular networks in three-dimensional tissue scaffolds and demonstrated their biofunctionality in vitro. We describe the lithographic technique used to form endothelialized microfluidic vessels within a native collagen matrix; we characterize the morphology, mass transfer processes, and long-term stability of the endothelium; we elucidate the angiogenic activities of the endothelia and differential interactions with perivascular cells seeded in the collagen bulk; and we demonstrate the nonthrombotic nature of the vascular endothelium and its transition to a prothrombotic state during an inflammatory response. The success of these microvascular networks in recapitulating these phenomena points to the broad potential of this platform for the study of cardiovascular biology and pathophysiology.tissue engineering | regenerative medicine | microfluidics | cancer | blood T he microvasculature is an extensive organ that mediates the interaction between blood and tissues. It defines the biological and physical characteristics of the microenvironment within tissues and plays a role in the initiation and progression of many pathologies, including cancer (1) and cardiovascular diseases (2, 3). Conventional planar cultures fail to recreate the in vivo physiology of the microvasculature with respect to three-dimensional (3D) geometry (lumens and axial branching points), and interactions of endothelium with perivascular cells, extracellular tissue and blood flow (4). Studies of the microvasculature in vivo allow only limited control of physical, chemical, and biological parameters influencing the microvasculature and present challenges with respect to observation (5). In vitro cultures that produce tubular vessels within 3D matrices will aid in elucidation of the roles of the microvasculature in health and disease. Important progress has been made toward this goal: Biologically derived or synthetic materials have been used to generate macrovessel tubes (6) and endothelialized microtubes (7); cellular self-assembly has been used to generate random microvasculature (8); microfabrication has been used to define complex geometries in hydrogels at the micro-scale (9); and distributions of cells and biochemical factors within 3D scaffolds (10). Of particular note, the group of Tien has pioneered the use of collagen to template the growth of vascular endothelium (7, 11) and demonstrated appropriate permeability (7), response to cyclic AMP (12), and differential properties as a function of the luminal shear stress and composition of the medium (13). Nonetheless, prior methodologies have been unable to produce endothelialized networks that can undergo substantial remodeling via angiogenesis; elucidate the ...

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Section: Resultsmentioning

confidence: 99%

In vitro microvessels for the study of angiogenesis and thrombosis

Zheng

Chen

Craven

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

781

842

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“…When CMs alone were used, cells attached on day 1 and then formed small clusters of cells at days 3 and 7 ( Figure 1). In contrast, when CMs were seeded together with ECs, cells formed interconnected linear networks, as commonly seen with ECs in 3-dimensional culture environments, 31 with increasing spatial organization from day 1 to day 7 ( Figure 1). To establish whether preformed endothelial networks enhanced the organization of myocytes, we also seeded ECs 1 day before myocytes were added.…”

Section: Ec-cm Interactions Affect Myocyte Reorganizationmentioning

confidence: 99%

Endothelial Cells Promote Cardiac Myocyte Survival and Spatial Reorganization

et al. 2004

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“…Due to constraints of space, only selected in vitro angiogenesis assays are described; descriptions of additional assays can be found in the following excellent reviews: (Auerbach et al, 2000;Auerbach et al, 2003;Eccles et al, 2005;Entschladen et al, 2005;Huerta et al, 2007;Renvoizé et al, 1998;Staton et al, 2004;Vailhé et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents

Goodwin¹

2007

Microvascular Research

251

208

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Blood vessels, either in insufficient numbers or in excess, contribute to the pathogenesis of many diseases. Agents that stimulate angiogenesis can improve blood flow in patients with ischemic diseases, whereas anti-angiogenic agents are used to treat disorders ranging from macular degeneration to cancer. In this review I describe in vitro assays that can be used to assess the activity of agents that affect angiogenesis. Means of quantifying endothelial cell matrix degradation, migration, proliferation, apoptosis and morphogenesis are discussed, as are embryoid body, aortic ring and metatarsal assays of vessel outgrowth. Strengths and limitations of these techniques are also addressed.

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In Vitro Models of Vasculogenesis and Angiogenesis

Cited by 313 publications

References 139 publications

In vitro microvessels for the study of angiogenesis and thrombosis

In vitro microvessels for the study of angiogenesis and thrombosis

Endothelial Cells Promote Cardiac Myocyte Survival and Spatial Reorganization

In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents

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