In vitro models of intestinal epithelium: Toward bioengineered systems

Creff, Justine; Malaquin, Laurent; Besson, Arnaud

doi:10.1177/2041731420985202

Cited by 38 publications

(32 citation statements)

References 119 publications

(222 reference statements)

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“…Organoids are defined as an in vitro 3D culture obtained from primary tissue, embryonic stem cells (ESCs) or IPSCs, that are capable of self-organization, multicell-complex and manifest similar organ functionality as the tissue of origin [ 108 , 109 ]. Organoids have been able to provide the ability to establish a well-controlled system via external manipulation and mimic vivo physiology, respectively.…”

Section: Current Approaches To Gi Bioengineeringmentioning

confidence: 99%

Tissue engineering of the gastrointestinal tract: the historic path to translation

2022

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The gastrointestinal (GI) tract is imperative for multiple functions including digestion, nutrient absorption, and timely waste disposal. The central feature of the gut is peristalsis, intestinal motility, which facilitates all of its functions. Disruptions in GI motility lead to sub-optimal GI function, resulting in a lower quality of life in many functional GI disorders. Over the last two decades, tissue engineering research directed towards the intestine has progressed rapidly due to advances in cell and stem-cell biology, integrative physiology, bioengineering and biomaterials. Newer biomedical tools (including optical tools, machine learning, and nuanced regenerative engineering approaches) have expanded our understanding of the complex cellular communication within the GI tract that lead to its orchestrated physiological function. Bioengineering therefore can be utilized towards several translational aspects: (i) regenerative medicine to remedy/restore GI physiological function; (ii) in vitro model building to mimic the complex physiology for drug and pharmacology testing; (iii) tool development to continue to unravel multi-cell communication networks to integrate cell and organ-level physiology. Despite the significant strides made historically in GI tissue engineering, fundamental challenges remain including the quest for identifying autologous human cell sources, enhanced scaffolding biomaterials to increase biocompatibility while matching viscoelastic properties of the underlying tissue, and overall biomanufacturing. This review provides historic perspectives for how bioengineering has advanced over time, highlights newer advances in bioengineering strategies, and provides a realistic perspective on the path to translation.

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Section: Current Approaches To Gi Bioengineeringmentioning

confidence: 99%

Tissue engineering of the gastrointestinal tract: the historic path to translation

2022

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“…They can achieve long-term culture and in vivo-like self-renewal and self-organization, representing cellular heterogeneity, physiological functionality, and morphology of organs. [161][162][163][164] The interface characteristics and functions of the all-aqueous structure MPRs that can support the construction of organoids in vitro include the following points. 1) The all-aqueous structure MPRs are considered an ideal biomimetic matrix or scaffold for the long-term cell culture, the guidance of stem cell differentiation, the construction of in vitro 3D tissue and organoid, owing to their inherent biocompatibility and chemical openness (Figure 9a,b).…”

Section: Organoid In Vitro Based On All-aqueous Structure Mprsmentioning

confidence: 99%

Microfluidic Particle Reactors: From Interface Characteristics to Cells and Drugs Related Biomedical Applications

Xin

et al. 2022

Adv Materials Inter

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with advanced functions, [6] can be attributed to two aspects. 1) The exquisite control and manipulation abilities of fluids at a microscale have reached a new height as droplet microfluidic technology is leaping forward. The precisely tunable interface characteristics of emulsion templates, determined by the optimization of fluids composition and interfacial complexity, contribute to the inherent interface properties of MPRs. [7][8][9][10] 2) The introduction of advanced materials expands the interface characteristics of the MPRs. Considerable natural polymers, synthetic polymers, and stimuli-responsive biomaterials have been employed continuously with the development of materials science. As a result, a series of performances possessed by the materials can significantly improve the inherent interface characteristics of the MPRs under a combination of the mentioned advanced materials. [11][12][13][14][15][16] Overall, researchers have stressed the improvement of the development process of interface characteristics and functions (such as substance encapsulation and controlled release) by providing novel technical innovations in every aspect to support biomedical clinical applications related to cells and drugs. Recently, the types, preparation methods, and biomedical applications of MPRs have been systematically presented in several excellent reviews. [3][4][5][8][9][10][17][18][19][20] The thermodynamic properties of liquid-liquid droplet reactors have been analyzed more specifically in some reviews. [7,17] However, the origin of interface characteristics of MPRs, as well as the function and cuttingedge cell-and drug-related biomedical applications determined by the interface characteristics, are deficient in a systematic summary. This review highlights that different emulsion templates endow the inherent interface characteristics of MPRs. Besides, the methods of converting emulsion templates into MPRs are summarized by introducing specific functional biomaterials, followed by the flexible and adjustable interface characteristics expanded by the mentioned materials. Moreover, the biomedical applications related to cells and drugs are analyzed based on the mentioned unique interface characteristics of the MPRs. Furthermore, the existing challenges regarding the current status are presented, and the subsequent development of the MPRs is illustrated from various perspectives (Scheme 1).

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“…Although human enterocytes retain important anatomical and biochemical characteristics during the in vitro culture, their use also has major drawbacks, due to their culture difficulties and limited viability, as well as their inability to form polarized epithelial monolayers typical of a tissue enterocyte that shows an apical and a basolateral surface. Therefore, human carcinogenic cell lines such as Caco-2 or HT-29, (as well as its mutant variant HT-29 MTX) [5], derived from a colorectal carcinoma [6,7], are routinely used to establish in vitro intestinal models for pharmacological and pharmacokinetic application to evaluate strategies for investigation drug absorption [8] or human goblet cell differentiation and mucin secretion [9,10].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effect of an O-2-Substituted (1-3)-β-D-Glucan Produced by Pediococcus parvulus 2.6 in a Caco-2 PMA-THP-1 Co-Culture Model

Notararigo

Varela

Otal

et al. 2022

IJMS

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Bacterial β-glucans are exopolysaccharides (EPSs), which can protect bacteria or cooperate in biofilm formation or in bacterial cell adhesion. Pediococcus parvulus 2.6 is a lactic acid bacterium that produces an O-2-substituted (1-3)-β-D-glucan. The structural similarity of this EPS to active compounds such as laminarin, together with its ability to modulate the immune system and to adhere in vitro to human enterocytes, led us to investigate, in comparison with laminarin, its potential as an immunomodulator of in vitro co-cultured Caco-2 and PMA-THP-1 cells. O-2-substituted (1-3)-β-D-glucan synthesized by the GTF glycosyl transferase of Pediococcus parvulus 2.6 or that by Lactococcus lactis NZ9000[pGTF] were purified and used in this study. The XTT tests revealed that all β-glucans were non-toxic for both cell lines and activated PMA-THP-1 cells’ metabolisms. The O-2-substituted (1-3)-β-D-glucan modulated production and expression of IL-8 and the IL-10 in Caco-2 and PMA-THP-1 cells. Laminarin also modulated cytokine production by diminishing TNF-α in Caco-2 cells and IL-8 in PMA-THP-1. All these features could be considered with the aim to produce function foods, supplemented with laminarin or with another novel β-glucan-producing strain, in order to ameliorate an individual’s immune system response toward pathogens or to control mild side effects in remission patients affected by inflammatory bowel diseases.

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In vitro models of intestinal epithelium: Toward bioengineered systems

Cited by 38 publications

References 119 publications

Tissue engineering of the gastrointestinal tract: the historic path to translation

Tissue engineering of the gastrointestinal tract: the historic path to translation

Microfluidic Particle Reactors: From Interface Characteristics to Cells and Drugs Related Biomedical Applications

Anti-Inflammatory Effect of an O-2-Substituted (1-3)-β-D-Glucan Produced by Pediococcus parvulus 2.6 in a Caco-2 PMA-THP-1 Co-Culture Model

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