In vitro migration of peritoneal and spleen cells and its inhibition in some avion species

The effect of suppression and cytotoxic drugs on dinitrochlorobenzene induced delayed type hypersensitivity in the chicken

“…Sampson et al (1975) and Salmon 1978) reported that glucocorticosteroids inhibited proliferation of human spleen suppressor cells. Separation of white cells from spleen by ficoll-diatrizoate removes granulocytes (Trifonov et al, 1977) and cells nonadherent to plastic are said to be mainly free of both B-cells and macrophages, leaving the major population to be T-cells (Shortmann, 1975;Greaves et al, 1976).…”

“…Blood lymphocytes/monocytes were obtained by centrifugation of 5 ml lots of citrated peripheral blood over 5 ml of a ficoll-diatrizoate gradient (d = 1.085) following the method described by Trifonov et al (1977). Spleen cells were obtained by removing the capsule and pushing the spleen through a 10 ml syringe into phosphate buffered saline (PBS) and filtering through nylon gauze, before centrifugation over the ficoll-diatrizoate gradient.…”

Characterization of immunity to Pasteurella multocida

“…The LMIF acts to inhibit the random migration of polymorphonuclear neutrophils. A number of different migration inhibition assays have been described for use with avian leukocytes (47,48,49,50,51). The migration inhibition test often correlates with skin test reactions.…”