In Vitro Micropropagation of Rose (Hybrid Rosa spp.) through Plant Tissue Culture Technique

Abstract

Roses are the most important cut flowers in the world. Tissue culture of rose has been improved since last twenty years, and exploited for various purposes from basic anatomical and physiological research to micropropagation from auxiliary buds, shoot tips, leaf explants, etc. Single bud nodal stem segments were surface sterilized and then cultured on MS medium supplemented with 30 g/l sugar, 6 g/l agar and different concentrations of BAP(1, 2, 3, 4 and 5mg/l). Among them, the most suitable concentration for shoot initiation and multiplication was 3mg/l BAP. And it also found that the best for shoot elongation occurred in MS medium supplemented with BAP 3 mg/l and GA3 1mg/l. The proliferated microshoots were transferred to root inducing media of half strength MS medium supplemented with 15 g/l sugar, 5 g/l agar and NAA (0.5, 1, 1.5, 2 mg/l). The best rooting was observed at NAA 1 mg/l concentration. The least response in root formation was found at NAA 0.5 mg/l supplementation on half strength MS media.

Optimizing Micropropagation Protocol of Rose (Rosa hybrida L.) cv. ‘Double Delight’

“…It was found that increasing BAP concentrations up to 3.0 mg/l improved the percentage of shoot proliferation (88%), with an average of 7.20 ± 0.80 regenerated shoots per culture and a mean length of 5.22 ± 0.47 cm (Table 4). These findings are consistent with the findings of Oo et al (2021) and Thi (2008) in the case of R. hybrida. They showed that MS medium supplemented with 3.0 mg/l BAP was found to be the optimum concentration for shoot proliferation.…”

“…Additionally, the results indicate that the single use of IBA performed significantly better than NAA and the combined effect of IBA and NAA on the rooting of micro-shoots. As opposed to this, Oo et al (2021) demonstrated substantial rooting in hybrid Rosa spp. on half-strength MS medium containing 15 g/l sugar and 1.0 mg/l NAA.…”

“…Similarly to this, Kajla et al (2018) and Brunda et al (2017) showed that MS medium containing 2.0 mg/l BAP was sufficient for direct shoot induction in the case of different cultivars of R. hybrid. Oo et al (2021), Aggarwal et al (2020) and Tirkey et al ( 2019) also found that BAP was the best cytokinin type growth regulator for direct shoot induction in different cultivars of R. hybrida. In this study, the interactive effect of BAP and NAA was determined to be less favorable than the single effect of BAP (Table 1).…”