In vitro micropropagation of huizache: Preliminary results

Abstract

The irrational exploitation of native species of arid and semi-arid zones of Mexico has created the need to carry out research work that contributes to the reforestation of those zones, so the aim of this research was to establish the methodology of scarification and in vitro multiplication of the huizache. For scarification, three treatments were used; T1: Immersion in hot water for 20 minutes; T2: immersion in sulfuric acid for 1 h and cut with the scalpel before seeding; T3: sand the seeds and place them in water for 12 h. Once the scarification process was completed, the seeds were disinfected with 2% sodium hypochlorite and placed in flasks containing water-agar. For the multiplication phase, Murashige and Skoog medium supplemented with sucrose 30 g L-1, phytagel 2.5 g L-1 and four treatments were used: T1: BAP 0.5 mg L-1+ kin 0.5 mg L-1+ Chitosan 60 mg L -1; T2: BAP 1 mg L-1+ kin 0.5 mg L-1+ Chitosan 60 mg L-1; T3: BAP 0.5 mg L-1+ kin 0.5 mg L-1; T4: BAP 1 mg L-1+ kin 0.5 mg L-1. As results, in the scarification phase, the best treatments were T2 and T3 with 100% germination, and in the multiplication phase, T4 favored a multiplication coefficient of 3 and greater height and number of leaves in the explants than the rest of the treatments. These results will allow establishing the in vitro methodology of this important species.