In vitro micropropagation of Acacia nilotica subsp. indica Brenan via cotyledonary nodes

Abstract

Cotyledonary node explants of Acacia nilotica subspecies indica Brenan, differentiated multiple shoots on Gamborg et al.' s medium (B5, Gamborg et al. 1968) supplemented with cytokinins like N(6)-benzyladenine, 6-(γ, γ-Dimethylallylamino)-purine, kinetin or zeatin. Of the four, BA supported maximum multiple shoot differentiation; the highest average number of shoots (6.3) per expiant was in 1.5 mg/l. The number of shoots was further enhanced by (i) using nodal explants of in vitro regenerated shoots as microcuttings, and (ii) repeated subculture of the original expiants (stumps) on the same medium after excising the shoots. Thus, over seven hundred shoots could be obtained from a single cotyledonary node explant. Individual shoots, when transferred to 2 mg/l indole-3-acetic acid augmented medium organised healthy roots in 100% cultures. Such test tube grown plantlets have been successfully transferred to soil, where they grow well up to eight weeks.

Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway

“…After 60 days, the green areas developed into shoot buds in a frequency of 5 to 6 buds/ 0.5g of callus. Frequencies similar to this were observed by Rai & Chandra (1988) in Dalbergia latifolia, Kumar (1992) in Bauhinia purpurea and Dewan et al (1992) in Acacia nilotica. The addition of the auxins 2,4-D (Carvalho, 1998) and IAA was tested in tissue culture of B. forficata alone and in combination with BAP with no improvement of the number of shoot buds observed (data not shown).…”

“…It has been observed for various legumes that high concentration of cytokinin stimulated callus and subsequently shoot bud formation (Malik & Saxena, 1992;Dorneles et al, 1994), increased their survival (Dorneles et al, 1994) but also inhibited shoot growth (Thomé et al 1995). Other studies with legumes in vitro (Dewan et al, 1992;Mohamed et al, 1992;Murthy et al, 1996) have determined the need of cytokinin for shoot differentiation and bud multiplication. The percentage of buds elongating into shoot regenerants was 68.2% in this work, compared to values of 81 and 94% obtained by Murthy et al (1996) with Cicer arietinum.…”

“…Organogenesis has been reported for some leguminous tree species such as Dalbergia sissoo (Kumar et al, 1991), Acacia nilotica subsp. indica (Dewan et al, 1992), Parkinsonia aculeata (Mathur & Mukunthakumar, 1992) and Sesbania grandifolia (Detrez et al, 1994). Although micropropagation and organogenesis have been reported for B. purpurea L. (Kumar, 1992), B. variegata (Mathur & Mukunthakumar, 1992) and B. vahlii Wight and Arnott (Upreti & Dhar, 1996), no report on shoot bud formation structures from tissue culture is available in the literature for Bauhinia species.…”

In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH-B1 through enhanced axillary branching

“…BAP 8.84 μM +IAA 5.7 μM was found to be most prolific combination of the treatments with regard to number of shoots, leaves and length of axillary shoots of in vitro established culture. Among the two cytokinins (BAP, Kinetin), BAP was found to be most effective cytokinin in inducing multiple shoot formation (Dewan et al, 1992). However, a combination of an auxin IAA with BAP augmented multiplication of shoots.…”

In vitro micropropagation of a tree legume adapted to arid lands Acacia tortilis subsp raddiana

“…A few reports are available on cell suspension cultures or plantlet regeneration from embryonic explants viz A albida (Duhoux and Davies, 1985). A auriculiformis (Mittal et al, 1989), A koa (Skolmen, 1978(Skolmen, , 1986), A senegal (Dave et al, 1980), A mangium (Galiana et al, 1991) and A nilotica (Dewan et al, 1992 [24][25][26][27][28][29][30] replicates, and cultures for multiplication and elongation were grown for at least 3 passages of 4 weeks duration using conditions similar to those described above except that cotyledons were removed from the multiple shoot buds prior to subculture in the second passage.…”