In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia)

Gray, D. J.; Benton, C. M.

doi:10.1007/bf00048199

Cited by 84 publications

(52 citation statements)

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“…These results are not unusual as similar stunting of shoots regenerated using TDZ has been reported in grapes (Gray and Benton, 1991) and in tree species such as Cassia (Parveen and Shahzad, 2010), Myrica rubra (Asghari et al, 2013) and Calophyllum inophyllum (Thengane et al, 2006). TDZ use has also been associated with failure of regenerated shoots to root well and general inhibition of rooting (Gray and Benton, 1991;Lu, 1993;Ranyaphia et al, 2011). As for meristic characters, there was less morphological difference between the regenerants and wild type seedlings (Table 3).…”

Section: Morphological Variationsupporting

confidence: 80%

“…Overall, inhibition of rooting was greater in in vitro plants regenerated indirectly from zygotic embryos than those regenerated directly from cotyledon explants, suggesting a possible role of the mode of regeneration in determination of rooting response. The poor root meristics of both types of regenerants can also be attributed to the reported inability of plants regenerated by TDZ to root well (Gray and Benton, 1991;Lu, 1993;Ranyaphia et al, 2011).…”

Section: Morphological Variationmentioning

confidence: 99%

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Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

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Somaclonal variation induced in vitro during tissue culture can be a problem in clonal micropropagation of elite plants. This study investigated the extent of morphological and genetic similarity or dissimilarity between Melia volkensii in vitro plants (somatic seedlings) obtained via somatic embryogenesis and normal seedlings. Comparisons were made between in vitro plants regenerated directly from cotyledon explants, indirectly from zygotic embryos and normal seedlings of the same parent trees. Regeneration was achieved using half MS medium supplemented with 0.05 mg/l thidiazuron. Shoots were elongated in half MS with 0.1 mg/l BAP plus 0.01 mg/l IAA then rooted in half MS with 0.1 mg/l IBA and 0.1 mg/l NAA. Six morphometric and five meristic characters were used for the morphological characterization. PCR-RAPD markers were used for assessment of genetic similarity or distance. Multivariate analysis using principal coordinates, cluster analysis, analysis of similarities (Anosim) and similarity percentages analysis (SIMPER) revealed significant dissimilarities (p< 0.0001) in morphometric and meristic characters between the in vitro plants and normal seedlings. However, significant similarity (p<0.01) was observed in the RAPD-genic characters of the in vitro plants and normal seedlings. Out of six morphometric traits, taproot length, internode length and shoot height were the most important sources of dissimilarity, cumulatively accounting for 72.37% of overall morphometric dissimilarity. Number of lateral roots was the single most important source of meristic dissimilarity, with 77.02% contribution. Plants regenerated directly from cotyledons were more similar to the normal seedlings in morphological and RAPD-marker characters than those regenerated indirectly from zygotic embryos. This study paves the way for identification of trait-specific RAPD markers for further characterization through sequence-characterized amplified regions (SCARs).

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Section: Morphological Variationsupporting

confidence: 80%

Section: Morphological Variationmentioning

confidence: 99%

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

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“…Para induzir o crescimento dos explantes in vitro, recomenda-se a adição de uma citocinina ao meio de cultura inicial (BOUQUET; TORRE-GROSA, 2003;MACHADO et al, 2006), sendo a 6-benzilaminopurina (BAP) a mais utilizada e a mais efetiva para um grande número de cultivares (BIASI et al, 1998a;NOVÁK;JUVOVÁ, 1983;BENTON, 1990).…”

Section: Introductionunclassified

Indução de multibrotação in vitro em videira cv. Bordô

Ayub

Spinardi²,

Basso³

et al. 2010

Rev. Bras. Frutic.

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A micropropagação possibilita a propagação massal e pode contribuir para atender à demanda de plantas-matrizes e mudas de qualidade genética e sanitária comprovadas de videira. Diferentes meios de cultura, acrescidos ou não de reguladores vegetais, tem sido usado com freqüência para auxiliar na indução de brotações. O trabalho teve por objetivo avaliar os meios de cultura MS e GZ completo ou com metade da concentração de sais acrescidos de BAP para indução de multibrotação em videira da cv. Bordô. O experimento foi realizado em delineamento inteiramente casualizado, em esquema fatorial 4 x 4, com quatro meios de cultura (MS, MS/2, GZ, GZ/2) e quatro concentrações de BAP (0; 2,5; 5 e 10 μM), com quatro repetições. Avaliaram-se aos 30 dias o número de brotos por micro-estaca, número de novas microestacas por broto, número de folhas por broto, comprimento médio dos brotos, número de raízes por microestaca e comprimento da raiz principal. O meio de cultura mais eficiente para indução de multibrotação em videira cv. Bordô e o desenvolvimento da parte aérea é o meio MS acrescido de 2,5 μM de BAP.

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“…(Pires & Biasi, 2003). Diversos trabalhos têm apontado as técnicas de micropropagação como alternativa para a rápida propagação de cultivares da espécie V. rotundifolia e, especialmente, híbridos de Vitis e Muscadinia (Cao, 1990;Compton & Gray, 1994;Gray & Benton, 1990;Gray & Fisher, 1985;Meyerson et al, 1994;Sudarsono & Goldy, 1991;Wetzstein & Myers, 1994).…”

Section: Introductionunclassified

Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia)

et al. 2006

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RESUMOA micropropagação é uma técnica que possibilita a propagação massal de plantas de interesse econômico. Objetivou-se com este trabalho avaliar o efeito de 6-benzilaminopurina (BAP) e cinetina (CIN) na multiplicação do porta-enxerto de videira VR043-43 . O isolamento foi realizado em meio de cultura QL, suplementado com BAP ou CIN, nas concentrações de 0; 2,5; 5,0 e 10,0µM. Os subcultivos foram realizados a cada 45 dias. O número de brotações por explante aumentou ao longo dos subcultivos com BAP. Esse efeito não foi observado com a CIN, que obteve resultado semelhante ao da testemunha (1,0 brotação/explante). As concentrações de 5,0 e 10,0 µM de BAP promoveram o maior número de brotações, porém com redução da altura. A concentração mais elevada de CIN (10,0 µM), também teve efeito negativo na altura das brotações, assim como no número de folhas por brotação. A formação de calo (100%) foi observada com BAP e CIN em todas as concentrações, e na ausência de citocinina não houve a formação de calo. A citocinina BAP reduziu a formação de raízes, que foi de 100% com a CIN e a testemunha.Termos para indexação: Citocinina, micropropagação, enraizamento, Vitis. ABSTRACTThe micropropagation is one technique that makes possible the massal propagation of plants of economic interest. The objective of this work was to evaluate the effect of 6-benzilaminopurina (BAP) and kinetin (KIN) in the multiplication in vitro of the grapevine rootstock VR043-43 . The isolation was carried through in culture medium QL, supplemented with BAP or KIN, in the concentrations of 0; 2,5; 5,0 and 10,0µM. The subcultures had been carried out each 45 days. The number of shoots per explant increased along with the subcultures with BAP. However this effect was not observed with the KIN that it got resulted similar to the one of the cytokin-free (1,0 shoot/explant). The concentrations of 5,0 nd 10,0 µM of BAP had promoted the biggest number of shoots with reduction of the height. The highest concentration of KIN (10,0 µM) also had negative effect in the height of the shoots, as well as, in the number of leaves per shoot. The callus formation (100%) was observed with BAP and KIN in all the concentrations, and in the cytokinin absence it did not have the callus formation. Cytokinin BAP reduced the formation of roots that was of 100% with the KIN and cytokinin-free.

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In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia)

Cited by 84 publications

References 8 publications

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Indução de multibrotação in vitro em videira cv. Bordô

Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia)

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