In vitro micrografting and the histology of graft union formation of selected species of prickly pear cactus (Opuntia spp.)

Estrada-Luna, Andrés Adolfo; López-Peralta, Cristina; Cárdenas-Soriano, Elizabeth

doi:10.1016/s0304-4238(01)00296-5

Cited by 52 publications

(45 citation statements)

References 11 publications

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“…Evidence of the compatibility between rootstock and scion during micrografting is the high percentage of success of grafting, and the establishment of the vascular tissue continuity observed in the histological study [24]. This agrees with the results obtained in our study.…”

Section: Anatomy Of the Graft Union Formationsupporting

confidence: 93%

In Vitro Micrografting of Mature Carob Tree (Ceratonia siliqua L.)

Hsina¹,

Mtili²

2009

TOHORTJ

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A micrografting technique was developed for Ceratonia Siliqua using in vitro germinated seedlings as rootstocks and apex or shoot cultures (established from mature female tree source) as microscions. In vitro germinated seedlings were decapitated and used as rootstock. Mature explants were initiated on Murashige and Skoog medium containing 30 g/l sucrose, and 0.5 mg/l 6-benzylaminopurine, then transferred on the multiplication medium (Murashige and Skoog + 0.5 mg/l 6-benzylaminopurine + 0.1 mg/l gibberellic acid + 0.1 mg/l indol-3-butyric acid). Micrografts could be easily cultured on the same medium and developed after one month. Grafting success has been dependent on the method of grafting. The micrografting on hypocotyl (central grafting) method was significantly more successful (92, 50%) than the other grafts methods. The compatible graft union was observed one month after grafting. The histological cuttings showed that the graft union formation was initiated by the development of the callus bridge at the interface between scion and rootstock, after that, new vascular elements including vessels and tracheids were seen across the interface zone.

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Section: Anatomy Of the Graft Union Formationsupporting

confidence: 93%

In Vitro Micrografting of Mature Carob Tree (Ceratonia siliqua L.)

Hsina¹,

Mtili²

2009

TOHORTJ

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“…Healing is capable of initiating such cellular responses by triggering various intracellular signaling events (León et al 2001;Minibayeva et al 2015;Sophors et al 2016). Grafting generates an impulse to elicit healing mechanism that generates biological response (isolation layer formation, callus differentiation, callus proliferation and connection, cambium differentiation and connection, and conducting tissue differentiation and connection) (Estrada-Luna et al 2002;Fan et al 2015). Studies conducted on graft-healing mechanism focused on determining the quantities of various biochemical substances in the scion (Pina and Errea 2005;Aloni et al 2008;Muneer et al 2016), enzyme activity (Zarrouk et al 2010), and endogenous hormone content (Aloni et al 2008;van Hooijdonk et al 2011;Yin et al 2012), all of which vary with the healing stage; however, signaling pathways of grafting healing was seldom reached.…”

Section: Introductionmentioning

confidence: 99%

Signaling pathway in development of Camellia oleifera nurse seedling grafting union

Feng

Yang

Chen

et al. 2017

Trees

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Key message The anatomical and physiological signaling pathways associated with successful scion-rootstock union in nurse seedling grafting of Camellia oleifera propagation are illustrated. Abstract Grafting, the successful union between scion and rootstock, has practical and biological importance. Nurse seedling grafting, as those practiced for Camellia oleifera, often results in high cell division activity and affinity, and is usually associated with significant rootstock and scion anatomical structures changes. However, a comprehensive explanation of signaling pathways, and how they affect graft union development, is still largely unknown. The present study investigates the union formation process in C. oleifera nurse seedling grafts and determines that it consists of six stages, namely, isolation layer formation, rootstock callus differentiation, scion callus differentiation, callus proliferation and connection, cambium differentiation and connection, and conducting tissue differentiation and connection, extending over a period of 35 days. Principal components analyses of the observed changes in physiology and protein expression identified three main factors contributing to the union formation process: cell proliferation, cell differentiation, and vascular bundle development. Further analysis showed that the regulation of the union formation process can be divided into two signaling pathways, namely, calcium and MAPK, which occur during vascular bundle development and cell proliferation and differentiation, respectively.

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“…Para el establecimiento de un sistema de propagación in vitro en cactáceas se pueden emplear diversos tejidos u órganos como explantes, cuya elección depende del género y la especie. Se pueden usar brotes terminales de plántulas (Gómez et al, 2006), secciones laterales o verticales de cladodios (Estrada et al, 2002); aréolas simples (Pérez y Dávila, 2002;Ramírez et al, 2007;Quiala et al, 2009);semillas (Dávila et al, 2005;De Madeiros et al, 2006) y brotes florales (Wyka et al, 2006), entre otros, los cuales son cultivados con diferentes reguladores de crecimiento (auxinas, citocininas y giberelinas) de acuerdo con los objetivos de estudios y las respuestas morfogénicas deseadas.…”

Section: Introductionunclassified

“…Terminal shoots of seedlings can be used (Gómez et al, 2006), lateral or vertical sections of cladodes (Estrada et al, 2002); simple areolas (Pérez y Dávila, 2002;Ramírez et al, 2007;Quiala et al, 2009);seeds (Dávila et al, 2005;De Madeiros et al, 2006) and flower buds (Wyka et al, 2006), among others, which are grown with different growth regulators (auxins, cytokinins and gibberellins) in accordance with the study objectives and desired morphogenic responses.…”

Section: Introductionmentioning

confidence: 99%

Morfogénesis in vitro de Mammillaria plumosa Weber

Téllez-Román

López-Peralta

Hernández-Meneses

et al. 2017

Remexca

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Mammillaria plumosa es una cactácea mexicana altamente apreciada como planta ornamental por su peculiar morfología. La extracción desmedida y el saqueo de sus poblaciones silvestres han forzado su protección y actualmente se encuentra clasificada como una especie en peligro de extinción. Ante esta crítica situación el presente trabajo se planteó como objetivo desarrollar un sistema de propagación in vitro eficiente factible de implementarse como una de las estrategias para la recuperación de la especie. Segmentos de tallos con aréolas se sembraron en medio MS (1962), suplementado con 2,4-D (9, 13.5 y 18 μM) en combinación con cinetina (4.6, 9.3 y 13.9 μM). Todos los tratamientos evaluados indujeron la formación de callos pero la activación de las aréolas para su conversión en brotes solo se obtuvo con 18 μM de 2,4-D y 9.3 μM de cinetina. En la etapa de proliferación los callos continuaron su crecimiento en las mismas concentraciones de reguladores de crecimiento, la diferenciación de brotes se produjo a partir de la activación areolar y la diferenciación de brotes adventicios de novo. Los brotes formaron raíces de forma natural en el proceso pero el enraizamiento se mejoró con el cultivo en medio MS (1962) a la mitad de concentración de sales. En la aclimatación de plantas la tasa de supervivencia fue 85% en sustrato de turba y arena de río. Con este protocolo es posible establecer la micropropagación de Mammillaria plumosa donde se pueden regenerar un promedio 500 plantas en 24 semanas de cultivo, las cuales podrían servir en la restauración de poblaciones silvestres o para su aprovechamiento comercial.

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In vitro micrografting and the histology of graft union formation of selected species of prickly pear cactus (Opuntia spp.)

Cited by 52 publications

References 11 publications

In Vitro Micrografting of Mature Carob Tree (Ceratonia siliqua L.)

In Vitro Micrografting of Mature Carob Tree (Ceratonia siliqua L.)

Signaling pathway in development of Camellia oleifera nurse seedling grafting union

Morfogénesis in vitro de Mammillaria plumosa Weber

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