In vitro mechanistic and in vivo anti-tumor studies of Glycosmis pentaphylla (Retz.) DC against breast cancer

Shoja, Muhammed Haneefa; Reddy, Neetinkumar D.; Nayak, Pawan G.; Biswas, Subhankar; Srinivasan, Kannupal; Rao, C. Mallikarjuna

doi:10.1016/j.jep.2016.04.002

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…Our study is the first to evaluate R. stricta extracts (RSF EtOAc) using an animal model. In this study, tumors were observed in the 8 th week, in agreement with studies that reported that DMBA administration to female rats caused primary breast tumors within 2 to 3 months [32,33]. The histopathology of the tissue examined revealed little improvement in the tissue when compared to control.…”

Section: Discussionsupporting

confidence: 91%

Apoptotic Induction and Anti-Migratory Effects of Rhazya Stricta Fruit Extracts on a Human Breast Cancer Cell Line

et al. 2019

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Rhazya stricta is a medicinal plant that is widely used in Saudi folklore medicine for treatment of various diseases. R. stricta fruit powder was sequentially extracted with n-hexane, chloroform, ethyl acetate, and methanol using a Soxhlet extractor. The cytotoxic effects of these fractions on human breast cancer cells (MDA-MB-231 and MCF-7) and non-tumorigenic control cells (MCF-10A) were evaluated via cell viability measurements, microscopy, gene expression, and migration assays. Moreover, the effect of the most promising extract on 7,12-dimethyl-benz[a]anthracene (DMBA)-induced breast cancer was investigated in rats. The promising extract was also subjected to gas chromatography–mass spectrometry. Fruit extracts of R. stricta were significantly cytotoxic toward all tested cell lines, as demonstrated by MTT and LDH assays. Treatment of MDA-MB-231 cells with fruit ethyl acetate fraction (RSF EtOAc) increased expression 11of P53, Bax and activation of caspase 3/7. A cell migration scratch assay demonstrated that extracts at non-cytotoxic concentrations exerted a potent anti-migration activity against the highly invasive MDA-MB-231 cell line. Moreover, RT-PCR results showed that RSF EtOAc significantly downregulated MMP-2 and MMP-9 expression, which play an important role in breast cancer metastasis. Histological studies of breast tissue in experimental animals showed a slight improvement in tissue treated with fruit ethyl acetate extract. GC-MS chromatogram showed thirteen peaks with major constituents were camphor, trichosenic acid and guanidine. Our current study demonstrates that fruit extracts of R. stricta are cytotoxic toward breast cancer cell lines through apoptotic mechanisms.

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Section: Discussionsupporting

confidence: 91%

Apoptotic Induction and Anti-Migratory Effects of Rhazya Stricta Fruit Extracts on a Human Breast Cancer Cell Line

et al. 2019

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“…Previously reported methods were executed for the animal experiments and evaluation of different parameters. 19 Drug administration began when tumors became stable and clearly palpable.…”

Section: Experimental Designmentioning

confidence: 99%

Antiproliferative Potential of Ethyl acetate Extract of Clerodendrum thomsoniae Balf.f. on DMBA-induced Breast Cancer in Female Sprague-dawley Rats

Ashraf¹,

Kalaichelvan²,

Ragunathan³

et al. 2021

IJPER

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Background: Clerodendrum is a genus of around 500 species that has a place with the family Lamiaceae. Numerous types of this class have been demonstrated for the treatment of different maladies including cancer. This study aimed to investigate the anti-cancer activity of ethyl acetate fractions of Clerodendrum thomsoniae Balf.f. (EACT) in female Sprague-Dawley rats. Methods: 7, 12-dimethylbenz (a) anthracene (DMBA) was used to induce breast cancer. Two doses of (25 and 50 mg/kg) EACT were used for the study. Various biochemical, hematological and, antioxidant parameters were analyzed. Results: Reduction in mean tumor volume and tumor weight was observed in EACT treated groups. The blood parameters were seen as normal. In both DMBA and doxorubicin groups, malondialdehyde were increased and the level was significantly reduced in EACT-treated groups. The effect of catalase has been seen as reduced in the groups administered by DMBA and doxorubicin and normal in the EACT groups. In the DMBA control groups, nitrate and nitrite were found to increase, the levels in others were normal. There were less necrosis and infiltration in breast tissues treated with doxorubicin as well as in EACT. The curative effect was found to be dose-dependent in animals treated with EACT. Some of the damaged breast patterns were restored to normal by the treatments. GC-MS analysis confirmed 2, 4-bis(1-phenylethyl)-phenol (39%), which was found to be the major compound present in EACT. Conclusion: The study concludes that C. thomsoniae may be a potential anticancer candidate.

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“…DC. have many pharmacological effects including anti-inflammatory, antibacterial, and anticancer properties [32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

The Inhibitory Effect of KerraTM, KSTM, and MinozaTM on Human Papillomavirus Infection and Cervical Cancer

Choowongkomon,

Choengpanya,

Pientong

et al. 2023

Medicina

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Background and Objectives: Cervical cancer is one of the most common types of frequently found cancers in Thailand. One of the causative agents is the infection of the high-risk human papillomavirus (HPV) type 16 and 18. Traditional medicines are rich sources of bioactive compounds which are a valuable source for the development of novel cancer therapies. In this study, the therapeutic effects of 3 traditional medicines, KerraTM, KSTM, and MinozaTM, were studied on HeLa and CaSki cells. Materials and Methods: The effects of KerraTM, KSTM, and MinozaTM on cancer cells were evaluated through cytotoxicity and cell death assays. The infection assay using HPV-16 pseudovirus was also carried out. Results: All traditional medicines efficiently suppressed cell growths of HeLa and CaSki, with KerraTM being the most potent anticancer agent followed by KSTM and MinozaTM. KerraTM at 158 µg/mL and 261 µg/mL significantly increases the percentage inhibition of the HPV-16 pseudovirus infection in a pre-attachment step in a dose-dependent manner, while KSTM at 261 µg/mL efficiently inhibited viral infection in both pre-attachment and adsorption steps. However, KerraTM, KSTM, and MinozaTM at subtoxic concentrations could not reduce the viral E6 mRNA expressions of HPV-16 and HPV-18. Cell death assay by acridine orange/ethidium bromide showed that KerraTM increased population of dead cells in dose-dependent manner in both CaSki and HeLa. The percentage of secondary necrosis in KerraTM-treated CaSki was higher than that of HeLa cells, while the percentage of late apoptotic cells in HeLa was higher than that of CaSki, indicating that HeLa was more susceptible to KerraTM than CaSki. For KSTM and MinozaTM, these extracts at 250 µg/mL promoted autophagy over cell death. At 500 µg/mL, the percentage of dead cells in KerraTM was higher than that of KSTM and MinozaTM. Conclusions: KerraTM is a potent traditional medicine for promoting cancer cell death. KerraTM is possibly useful in the prevention and treatment of cervical cancer. Further investigation will be carried out to gain a better understanding of the biochemical mechanism and the pharmacological activity underlying this effect.

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In vitro mechanistic and in vivo anti-tumor studies of Glycosmis pentaphylla (Retz.) DC against breast cancer

Cited by 8 publications

References 28 publications

Apoptotic Induction and Anti-Migratory Effects of Rhazya Stricta Fruit Extracts on a Human Breast Cancer Cell Line

Apoptotic Induction and Anti-Migratory Effects of Rhazya Stricta Fruit Extracts on a Human Breast Cancer Cell Line

Antiproliferative Potential of Ethyl acetate Extract of Clerodendrum thomsoniae Balf.f. on DMBA-induced Breast Cancer in Female Sprague-dawley Rats

The Inhibitory Effect of KerraTM, KSTM, and MinozaTM on Human Papillomavirus Infection and Cervical Cancer

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