In vitro maturation of bovine oocytes: beneficial effects of cysteamine

Sandal, Asiye İ̇zem; Özdaş, Özen Banu; Baran, Alper

doi:10.15406/jdvar.2018.07.00191

Cited by 3 publications

(2 citation statements)

References 23 publications

(30 reference statements)

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“…Although cysteamine in concentration 50 µm could not increase nuclear maturation, however it is presumable could increase oocyte quality, thus improve fertilization rate. These statement is supported by Sandal et al (2018) that cysteamine can increase GSH content to protect the cell, as well as increasing cell quality.…”

Section: Discussionmentioning

confidence: 82%

Cysteamine in Maturation Medium Enhances Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro

2020

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Low nuclear maturation and fertilization rate is one obstacle in the in vitro embryo production which decrease embryo yield. This problem is presumable related with high production of reactive oxygen species (ROS) during maturation process. Glutathione (GSH) as an antioxidant is well known to overcome effect of ROS production. GSH synthesis in the cytosol part of the oocyte cytoplasm is influenced by cysteine availability. It is therefore, this research was conducted to evaluate the ability of cysteamine to provide cysteine availability as GSH precursor on the nuclear maturation and fertilization rate of sheep oocytes. Results of this experiment revealed additional cysteamine at 150 µm and 200 µm could significantly improve nuclear maturation rate. On the other side, although additional of cysteamine at 50 µm could not improve nuclear maturation rate, however 50 µm cysteamine in the maturation medium could significantly improve the fertilization rate. Based on those experiment results, it seems that the additional cysteamine might be improve not only GSH availability but also the oocyte quality which characterized the ability of pronuclear formation. This finding strongly suggested that additional cysteamine in the maturation medium could improve nuclear maturation and fertilization rate of sheep oocytes.

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Section: Discussionmentioning

confidence: 82%

Cysteamine in Maturation Medium Enhances Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro

2020

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“…Thus, the enzymatic antioxidants comprise catalase (CAT) and superoxide dismutase stress (SOD) while the non-enzymatic antioxidants comprising glutathione (GSH), cysteamine, vitamin C, and vitamin E have been used to supplement the culture medium to reduce the intracellular level of ROS (Caamano et al, 1998;He et al, 2017). GSH functions protection against oxidative damage, raising embryo development competence (Sandal et al, 2018). Cysteine and cysteamine are precursors of GSH.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cysteine Supplementation in Maturation Medium on Bovine Embryos Development

Amporn¹,

Guntaprom²,

Duongmawong³

et al. 2023

AAVS

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This research study investigated cysteine supplements and their effects upon in vitro maturation (IVM) media. Experimentally, cumulus-oocyte complexes (COCs) which were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml cysteine where 0 served as the control. IVF-TALP medium was used to fertilize the mature oocytes which subsequently underwent culturing in a simple culture medium (KSOM). The results showed that percentage of matured oocytes, fertilized oocytes, 8-16 cells developments, blastocyst in 100 μM cysteine group (73.00%, 54.79%, 30.14%, and 17.81%, respectively) were higher than the control group (48.00%, 37.50%, 18.75%, and 10.42%, respectively) and 200 μM cysteine (respectively, 52.00%, 38.46%, 19.23%, and 9.62%) at a significance level of 0.05, but the difference between the 200 μM cysteine group and the control was not significant (p>0.05). The findings confirm that adding 100 μM cysteine to IVM media significantly increased the percentage of matured oocytes, fertilized oocytes, 8-16 cells developments and blastocyst stage.

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Current Advances in Bovine In Vitro Maturation and Embryo Production Using Different Antioxidants: A Review

Naspinska,

Moreira da Silva,

Moreira da Silva

2023

JDB

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In vitro maturation (IVM) is one of the most important steps in in vitro embryo production (IVEP). It is a complicated procedure in which nuclear and cytoplasmatic changes in oocytes appear. In order to carry out the in vitro maturation procedure correctly, it is necessary to provide the oocytes with as close to a natural (in vivo) environment as possible. Many factors contribute to the overall poor quality of in vitro-matured oocytes. One important factor may be oxidative stress (OS). The generation of oxidants, such as reactive oxygen species, is common under culture conditions. The solution for OC treatment and prevention is antioxidants. In the last 5 years, many studies have examined different antioxidants and their effects on in vitro maturation of oocytes and embryo production. The aim of this systematic review was to present the achievements of scientific research in the last five years, in which the effects of many antioxidants were tested on bovine oocyte maturation and embryo production.

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In vitro maturation of bovine oocytes: beneficial effects of cysteamine

Cited by 3 publications

References 23 publications

Cysteamine in Maturation Medium Enhances Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro

Cysteamine in Maturation Medium Enhances Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro

Effect of Cysteine Supplementation in Maturation Medium on Bovine Embryos Development

Current Advances in Bovine In Vitro Maturation and Embryo Production Using Different Antioxidants: A Review

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