This research study investigated cysteine supplements and their effects upon in vitro maturation (IVM) media. Experimentally, cumulus-oocyte complexes (COCs) which were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml cysteine where 0 served as the control. IVF-TALP medium was used to fertilize the mature oocytes which subsequently underwent culturing in a simple culture medium (KSOM). The results showed that percentage of matured oocytes, fertilized oocytes, 8-16 cells developments, blastocyst in 100 μM cysteine group (73.00%, 54.79%, 30.14%, and 17.81%, respectively) were higher than the control group (48.00%, 37.50%, 18.75%, and 10.42%, respectively) and 200 μM cysteine (respectively, 52.00%, 38.46%, 19.23%, and 9.62%) at a significance level of 0.05, but the difference between the 200 μM cysteine group and the control was not significant (p>0.05). The findings confirm that adding 100 μM cysteine to IVM media significantly increased the percentage of matured oocytes, fertilized oocytes, 8-16 cells developments and blastocyst stage.
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