In vitro maturation and early developmental capacity of bovine oocytes cultured in pure follicular fluid and supplementation with follicular wall

Coleman, Natalia; Shagiakhmetova, Galina A.; Lebedeva, I.; Kuzmina, T. I.; Golubev, A. K.

doi:10.1016/j.theriogenology.2006.10.019

Cited by 21 publications

(21 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In horses, Dell 'Aquila et al (1997) and Bøgh et al (2002), using 100% FF, found nuclear maturation rates of 68.5 and 79%, respectively. In the present study, pure FF provided lower rates, ranging from 43 to 65%, similar to the reports of Avery et al (2003) and Coleman et al (2007), who describe nuclear maturation rates of 41 and 44%, respectively, in cattle.…”

Section: Resultssupporting

confidence: 92%

“…Follicular fluid as a maturation medium is sufficient to promote nuclear maturation; however, it presents limitations to support appropriate embryo development (Avery et al, 2003;Coleman et al, 2007). Due to the inhibitory effect on germinal vesicle breakdown and on meiotic progression, FF leads to a delay on the nuclear maturation (Ducolomb et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

View full text Add to dashboard Cite

-This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 -100% FF) slowed meiotic progression and CG migration (control -78.13% vs. treated -52.58% and control -52.7% vs. treated -11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control -90.13% vs. treated -82.64% and control -35.73% vs. treated -11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control -29.91 vs. treated -35.49, at 24 h of maturation) and total cell numbers (control -109.53 vs. treated -120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Moreover, maturation of oocytes in the FF did not have any deleterious effects on oocyte developmental competence, as the proportion of oocytes that cleaved and developed to the blastocyst stage did not differ from that of oocytes matured in the standard maturation medium. Similarly, previous studies have reported that oocyte maturation in FF or addition of a certain concentration of FF can even improve embryonic development (Ali et al 2004, Coleman et al 2007. By contrast, another study showed that maturation of oocytes in FF reduces developmental competence (Avery et al 2003).…”

Section: Discussionmentioning

confidence: 69%

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Asaf

Leitner²,

Furman³

et al. 2014

Reproduction

View full text Add to dashboard Cite

Mastitis is associated with decreased fertility in dairy cows. In the current study, we created an experimental model to simulate shortterm mastitis by a single intramammary administration of Gram-negative endotoxin of Escherichia coli origin (GK), or Gram-positive toxin of Staphylococcus aureus origin (GC), to examine the effect of mastitis on oocyte developmental competence. Healthy Holstein cows were synchronized, and follicular fluid (FF) of cows treated with GC or GK and of uninfected cows (controls) was aspirated from the preovulatory follicles by transvaginal ultrasound procedure. The aspirated FF was used as maturation medium for in vitro embryo production. The distribution of matured oocytes into different cortical granule classes and meiotic stages was affected by GK administration (P!0.05) but not by GC administration. The proportion of oocytes that cleaved to two-and four-cell stage embryos (44 h postfertilization) was lower in both GC and GK groups than in controls (P!0.05). Blastocyst formation rate (7-8 days postfertilization) was lower in the GK group (P!0.05) and numerically lower in the GC group compared with their uninfected counterparts. The total cell number in blastocysts did not differ among groups; however, the apoptotic index was higher in the GC group (P!0.05), but not in the GK group, relative to controls. Examining mRNA relative abundance in oocytes and early embryos revealed mastitis-induced alterations in PTGS2 (COX2), POU5F1, and HSF1 but not in SLC2A1 (GLUT1) or GDF9. Results indicate a differential disruptive effect of mastitis induced by GK and GC on oocyte developmental competence in association with alterations in maternal gene expression.

show abstract

“…In addition, Fetal Dromedary Camel Serum (FDCS) supplementation to the media shows better maturation rate which might be due to the compositions of FDCS serum which is composed of many immunoglobulins of the camel species (El-Hatmi et al 2006). In comparison with the results dealing with the in vitro maturation rate of camels oocytes, Coleman et al (2007) showed that the in vitro maturation rate of bovine oocytes matured in TCM-199 supplemented with fetal calve serum amounted to 62%. Whereas, the maturation rate was 53.8% for buffalo oocytes (Shamiah 2004).…”

Section: Discussionmentioning

confidence: 98%

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Abdel-Khalek¹,

Gabr²,

Khalil³

et al. 2017

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter). Meanwhile, Fetal Dromedary Camel Serum (FDCS) was obtained from camel fetuses after slaughtering. Thereafter, only Cumulus Oocyte Complexes (COCs) were matured in vitro in the Tissue Culture Medium (TCM-199) complemented with 10% FDCS. Spermatozoa required for in vitro fertilization were collected from testes (epididymal cauda) of the slaughtered camel bulls. The results clearly showed that the maturation rate of oocytes at metaphase II was about 59.5% while the fertilization rate was around 70.4%. Intriguingly, the embryo rates determined were 13.1%, in 2-cell; 0.0%, in 4-cell; 34.7%, in 8-16% cell; 39.1%, in morula and 13.1% in a blastocyst stage. This study represented a successful in vitro production of Sudanese dromedary camel embryos from epididymal sperm cells and in vitro matured oocytes recovered from slaughtered camels.

show abstract

In vitro maturation and early developmental capacity of bovine oocytes cultured in pure follicular fluid and supplementation with follicular wall

Cited by 21 publications

References 28 publications

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Contact Info

Product

Resources

About