In vitro lymphocyte transformation in atopic patients: Induced by antigens

Girard, Jean Pierre; Rose, Noel R.; Kunz, Marie L.; Kobayashi, Setsuo; Arbesman, Carl E.

doi:10.1016/0021-8707(67)90113-x

Cited by 92 publications

(33 citation statements)

References 16 publications

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“…These include antigen-induced lymphocyte transformation and measuring the elaboration of lymphocyte mediators (MIF and mitogenic factor). Other investigators have previously shown that lymphocytes from atopic patients with summer or-fall hayfever transformed and/ or incorporated increased amounts of [3H]thymidine in vitro (1)(2)(3)(4)(5) and produced mediators (4)(5)(6) in response to grass or ragweed pollen antigens. We found that lymphocytes from 22/28 patients with ragweed hayfever incorporated increased amounts of [3H]thymidine in response to RAgE.…”

Section: Discussionmentioning

confidence: 99%

“…To what extent components of cellular immunity (delayed hypersensitivity) are involved in this response, however, is not altogether clear. Since it is often difficult to elicit positive delayed hypersensitivity skin tests after the intradermal injection of pollen antigens, some investigators have elected to study in vitro lymphocyte responses to certain pollens as a correlate of in vivo hypersensitivity (1)(2)(3)(4)(5)(6). Some of the techniques which measure in vitro lymphocyte activation include the incorporation of ['H]thymidine into cellular DNA (7) and the detection of soluble mediators such as migration inhibitory factor (MIF)' and mitogenic (lymphocyte transforming) factor (8).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Rocklin¹,

Pence²,

Kaplan³

et al. 1974

J. Clin. Invest.

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A B S T R A C T The in vivo and in vitro responses to ragweed antigen E were evaluated in 28 untreated atopic patients with ragweed hayfever. The methods employed included direct skin testing, measurement of total serum IgE, measurement of specific IgE anti-ragweed antibodies, leukocyte histamine release, lymphocyte transformation, and release of lymphocyte mediators (migration inhibitory factor and mitogenic factor). The patients could be divided into sensitive and insensitive groups on the basis of their in vitro reactivity to antigen E. 20 patients in the sensitive group had statistically higher levels of total serum IgE, higher levels of specific IgE anti-ragweed antibodies, and greater leukocyte sensitivity as measured by antigen-induced histamine release than did eight patients in the insensitive group. Lymphocytes from sensitive patients produced greater amounts of migration inhibitory factor and mitogenic factor when challenged by antigen E than did lymphocytes from insensitive patients. A possible role for the lymphocyte in this allergic disease is discussed. serum IgE levels of paIgEAR levels but normal total serum IgE levels. Of interest was the finding that a small number of patients had diminished lymphocyte mediator production, histamine release, IgE, and IgEAR levels. One could not distinguish clinically between these two groups. METHODSPatients. 28 atopic patients with symptoms compatible with seasonal allergic rhinitis and no previous history of receiving ragweed immunotherapy were included in this study. The ages ranged from 15 to 64 yr (mean 28.9 yr) and included 23 males and 5 females. 18 nonatopic subjects served as controls.Skin tests. At one forearm site 0.05 ml of aqueous ragweed extract (100 protein nitrogen units/ml) alone was injected intradermally. In the opposite forearm 0.05 ml chlorpheniramine maleate (10 mg/ml) was injected before the subsequent intradermal injection of 0.05 ml ragweed extract at the same site. At a third site chlorpheniramine alone was injected (0.05 ml). The amount of erythema and induration were measured at each site at 15 min and 48 h. In one subject a punch biopsy specimen was obtained at 48 h from sites injected with ragweed extract plus chlorpheniramine and chlorpheniramine alone.Serum IgE levels. Serum samples were obtained from patients at the time of initial evaluation and IgE levels determined by use of a Phadebas kit purchased from Pharmacia (Uppsala, Sweden). Serum samples for IgE levels were also obtained from 50 subjects in the same age range as the patient population.Specific IgEAR levels. The radioallergosorbent test (RAST) was used to measure specific IgEAR levels (9). 10 ml of a 20% whole ragweed extract was conjugated to 500 mg of cyanogen bromide-activated cellulose particles (10). 25-, 50-, and 75-il samples of sera were added to 0.5-mg aliquots of the antigen-cellulose conjugate and incubated overnight in a horizontal rotator. After washing, 0.05 ml of "I-labeled immunosorbent purified anti-IgE (Fc) was added to each tube. The anti-IgE (...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Rocklin¹,

Pence²,

Kaplan³

et al. 1974

J. Clin. Invest.

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“…Control cultures were incubated without antigen but received AgE (50 ,ug/ml) at the end of the incubation period (R cultures). The (4)(5)(6). However, the relation between this cellular response and the in vivo allergic reaction remains to be studied.…”

Section: Methodsmentioning

confidence: 99%

“…In several species of experimental animals, T and B cell interaction is prerequisite for the production of reaginic antibody (IgE) (3). The role of this interaction in human IgE antibody response is not clear because experiments showing proliferation of lymphocytes exposed to allergens in vitro (4) with synthesis of macrophage migration inhibitory factor and mitogenic factor (5,6) were performed with unfractionated suspensions of lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Cooperation between human thymus-derived and bone marrow-derived lymphocytes in the antibody response to ragweed antigen E in vitro.

Geha¹,

Colten²,

Schneeberger³

et al. 1975

J. Clin. Invest.

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“…However, recent evidence suggests that cellular immune responses to ragweed antigen may also be detected (2)(3)(4)(5)(6)(7)(8). In our previous study (2) we measured a broad range of immunologic responses including antigen-induced leukocyte histamine release, the radioallergosorbent test (RAST)1 for specific IgE antiragweed antibody (IgEAR), lymphocyte proliferation, and the production of two lymphocyte mediators, migration inhibitory factor (MIF) and mitogenic factor (MF).…”

Section: Introductionmentioning

confidence: 99%

The effect of immunotherapy on humoral and cellular responses in ragweed hayfever.

Evans¹,

Pence²,

Kaplan³

et al. 1976

J. Clin. Invest.

140

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A B S T R A C T The effect of specific immunotherapy on several in vitro responses to ragweed antigen E has been evaluated in 17 atopic patients with ragweed hayfever. The methods employed were leukocyte histamine release, measurement of specific IgE anti-ragweed antibody and specific IgG anti-ragweed antibody, lymphocyte proliferation, and the production of two lymphocyte mediators (migration inhibitory factor and mitogenic factor). The duration of treatment and symptom improvement were also recorded for comparison.Immunotherapy was associated with a decrease in leukocyte sensitivity for histamine release to ragweed antigen E in a majority of the patients. In addition, there was a significant decrease in IgE anti-ragweed antibody and a significant increase in IgG anti-ragweed antibody. Immunotherapy also resulted in a significant decrease in lymphocyte responsiveness to ragweed antigen E as measured by proliferation and the production of mediators. Symptomatic improvement was best correlated with the presence of IgG anti-ragweed antibody responses. The production of this antibody was also associated with a decrease in lymphocyte responsiveness. The results of this study indicate that specific immunotherapy in ragweed-sensitive patients induces alterations in immunologic reactivity to ragweed antigen in vitro. This response is antigen specific, includes elements of both humoral and cellular immunity, and may account

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In vitro lymphocyte transformation in atopic patients: Induced by antigens

Cited by 92 publications

References 16 publications

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Cooperation between human thymus-derived and bone marrow-derived lymphocytes in the antibody response to ragweed antigen E in vitro.

The effect of immunotherapy on humoral and cellular responses in ragweed hayfever.

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