In Vitro Leukocyte Adhesion in Endothelial Tissue Culture Models Under Flow

Cooper, Scott; Dick, Melissa; Emmott, Alexander; Jonak, Paul; Rouleau, Léonie; Leask, Richard L.

doi:10.5772/19784

Cited by 2 publications

(2 citation statements)

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“…68,69 Tethering and rolling of leukocytes on ECs are controlled by selectins (E and P), adhesion is regulated by integrins, and transmigration is guided through the gap junctions by PECAM-1. Consequently, evaluating CAM expression in flow for ECs is central to understanding the nature of interaction of leukocytes with ECs.…”

Section: Flow-based In Vitro Models and Cams Expressionmentioning

confidence: 99%

Targeting cell adhesion molecules with nanoparticles usingin vivoand flow-basedin vitromodels of atherosclerosis

Khodabandehlou

Masehi-Lano

Poon

et al. 2017

Exp Biol Med (Maywood)

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Impact statementAs atherosclerosis remains the leading cause of death, there is an urgent need to develop better tools for treatment of the disease. The ability to improve current treatments relies on enhancing the accuracy of in vitro and in vivo atherosclerotic models. While in vivo models provide all the relevant testing parameters, variability between animals and among models used is a barrier to reproducible results and comparability of NP efficacy. In vitro cultures isolate cells into microenvironments that fail to take into account flow separation and shear stress, which are characteristics of atherosclerotic lesions. Flow-based in vitro models provide more physiologically relevant platforms, bridging the gap between in vivo and 2D in vitro models. This is the first review that presents recent advances regarding endothelial cell-targeting using adhesion molecules in light of in vivo and flow-based in vitro models, providing insights for future development of optimal strategies against atherosclerosis. AbstractAtherosclerosis is a leading cause of death worldwide; in addition to lipid dysfunction, chronic arterial wall inflammation is a key component of atherosclerosis. Techniques that target cell adhesion molecules, which are overexpressed during inflammation, are effective methods to detect and treat atherosclerosis. Specifically, research groups have identified vascular cell adhesion molecule-1, intercellular adhesion molecule-1, platelet endothelial cell adhesion molecule, and selectins (E-selectin and P-selectin) as correlated to atherogenesis. In this review, we discuss recent strategies both in vivo and in vitro that target cell adhesion molecules. First, we discuss peptide-based and antibody (Ab)-based nanoparticles utilized in vivo for diagnostic, therapeutic, and theranostic applications. Second, we discuss flow-based in vitro models that serve to reduce the traditional disadvantages of in vivo studies such as variability, time to develop the disease, and ethical burden, but preserve physiological relevance. The knowledge gained from these targeting studies can be translated into clinical solutions for improved detection, prevention, and treatment of atherosclerosis.

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Section: Flow-based In Vitro Models and Cams Expressionmentioning

confidence: 99%

Targeting cell adhesion molecules with nanoparticles usingin vivoand flow-basedin vitromodels of atherosclerosis

Khodabandehlou

Masehi-Lano

Poon

et al. 2017

Exp Biol Med (Maywood)

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“…Two‐dimensional models and consequence on cell attachment. (a) Schematic of a parallel‐plate flow chamber (from Cooper et al, ) (b) Top: fluorescence microscopy images showing cells attached either predominantly flatly to collagen struts (left) or in a bridged manner (right). Bottom: schematic diagram of attachment morphologies with flatly attached cells on the left and bridged (either dual or multiattachment points) cells on the right.…”

Section: Obstacles In Defining Optimal Flow Effectsmentioning

confidence: 99%

Strategy for achieving standardized bone models

Hadida

Marchat

2019

Biotech & Bioengineering

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Reliably producing functional in vitro organ models, such as organ‐on‐chip systems, has the potential to considerably advance biology research, drug development time, and resource efficiency. However, despite the ongoing major progress in the field, three‐dimensional bone tissue models remain elusive. In this review, we specifically investigate the control of perfusion flow effects as the missing link between isolated culture systems and scientifically exploitable bone models and propose a roadmap toward this goal.

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In Vitro Leukocyte Adhesion in Endothelial Tissue Culture Models Under Flow

Cited by 2 publications

References 61 publications

Targeting cell adhesion molecules with nanoparticles usingin vivoand flow-basedin vitromodels of atherosclerosis

Targeting cell adhesion molecules with nanoparticles usingin vivoand flow-basedin vitromodels of atherosclerosis

Strategy for achieving standardized bone models

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