In vitro lens capsule model for investigation of posterior capsule opacification

Cleary, Georgia; Spalton, David J.; Zhang, Jinjun; Marshall, John

doi:10.1016/j.jcrs.2010.05.006

Cited by 22 publications

(24 citation statements)

References 15 publications

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“…14,19 -23 With respect to testing IOLs using such systems, the body of literature is limited. 14,19,22,24 Liu et al 14 initially demonstrated that an IOL could impede growth to some degree using the human capsular bag system developed in Norwich. In the present study, we made a modification in the protocol such that the capsular bag containing the IOL was inverted, resulting in the anterior capsule being closest to the plastic dish.…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that pinning directly through the capsule can distort the capsular bag and, therefore, prevent the IOL locating in its natural position; however, using pins to secure the capsular bag has provided results that reflect clinical features of PCO, thus demonstrating the value of this approach. Another variant of the capsular bag system that was recently published by Cleary et al 19 retains the ciliary body, which is pinned to a silicon ring. Using this method, the capsular bag is effectively suspended and can house an IOL, which can also be observed.…”

Section: Discussionmentioning

confidence: 99%

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A Fully Human In Vitro Capsular Bag Model to Permit Intraocular Lens Evaluation

Dawes

Illingworth

Wormstone

2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To establish a fully human in vitro culture model with which to test the putative effects of intraocular lens (IOL) designs in preventing posterior capsule opacification (PCO) after cataract surgery. METHODS. A sham cataract operation was performed to prepare human capsular bags from donor lenses. In one capsular bag of a donor pair, an intraocular lens (PMMA round-edge IOL or acrylic IOL) was implanted while the other capsular bag remained aphakic. Bags were transferred to a Petri dish and secured anterior-face down using entomological pins. Capsular bags were maintained in Eagle's minimum essential medium supplemented with 2% human serum and 10 ng/mL TGF-␤ to drive growth and matrix contraction. RESULTS. In the absence of an IOL, cells appeared within the central posterior capsule at 4.38 Ϯ 0.26 days, whereas in the presence of a PMMA round-edge IOL or an acrylic IOL they appeared at 8 Ϯ 0.41 days and 11 Ϯ 0.7 days, respectively. Immunocytochemical analysis showed an accumulation of cells at the edge of the acrylic IOL and a less evident accumulation with the PMMA round-edge IOL. Moreover, matrix contraction was more prominent in the absence of an IOL but was still apparent, to a lesser degree, in the presence of a PMMA round-edge IOL. The acrylic IOL greatly suppressed matrix contraction. CONCLUSIONS. The authors have developed a fully human in vitro capsular bag system that relates well to clinical observations and permits the testing of novel intraocular lenses. (Invest Ophthalmol Vis Sci. 2012;53:23-29)

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Fully Human In Vitro Capsular Bag Model to Permit Intraocular Lens Evaluation

Dawes

Illingworth

Wormstone

2012

Invest. Ophthalmol. Vis. Sci.

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“…The development of capsular bag models allowed for the introduction of an IOL (Cleary et al, 2010;Dawes et al, 2012;De Groot et al, 2005;Duncan et al, 1997;Eldred et al, 2014;Liu et al, 1996) and thus provide an alternative experimental system for IOL development and importantly permit IOLs to be tested in a human system. For example, the capsular bag model served as an invaluable tool in the development of the Bag-in-the-lens IOL developed by Prof. Tassignon (De Groot et al, 2005), which is now commercially available.…”

Section: Capsular Bagsmentioning

confidence: 99%

“…Efforts were also being made to establish a suspended capsular bag system, such that ElOsta et al (2003) initially secured the periphery of the capsular bag to a lens holder using medical grade glue and this permitted IOLs to be introduced. An advance on this method was developed by Cleary et al (2010), which retained the ciliary body and zonular fibres in association with the capsular bag. The ciliary body is pinned to a silicon ring.…”

Section: Capsular Bagsmentioning

confidence: 99%

Experimental models for posterior capsule opacification research

Wormstone

Eldred

2016

Experimental Eye Research

103

102

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Millions of people worldwide are blinded due to cataract formation. At present the only means of treating a cataract is through surgical intervention. A modern cataract operation involves the creation of an opening in the anterior lens capsule to allow access to the fibre cells, which are then removed. This leaves in place a capsular bag that comprises the remaining anterior capsule and the entire posterior capsule. In most cases, an intraocular lens is implanted into the capsular bag during surgery. This procedure initially generates good visual restoration, but unfortunately, residual lens epithelial cells undergo a wound-healing response invoked by surgery, which in time commonly results in a secondary loss of vision. This condition is known as posterior capsule opacification (PCO) and exhibits classical features of fibrosis, including hyperproliferation, migration, matrix deposition, matrix contraction and transdifferentiation into myofibroblasts. These changes alone can cause visual deterioration, but in a significant number of cases, fibre differentiation is also observed, which gives rise to Soemmering's ring and Elschnig's pearl formation. Elucidating the regulatory factors that govern these events is fundamental in the drive to develop future strategies to prevent or delay visual deterioration resulting from PCO. A range of experimental platforms are available for the study of PCO that range from in vivo animal models to in vitro human cell and tissue culture models. In the current review, we will highlight some of the experimental models used in PCO research and provide examples of key findings that have resulted from these approaches.

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“…The model previously described by Liu et al 13 and developed by Cleary et al 14 was employed. Pairs of donor eyes (corneas previously removed for eye banking; Table) were obtained within 48-hours post mortem with national research ethics committee approval and used in accordance with the tenets of the Declaration of Helsinki.…”

Section: Capsular Bag Preparationmentioning

confidence: 99%