In Vitro Investigations of Chromium- and Nickel-Hypersensitivity with Culture of Skin and Peripheral Lymphocytes

Detection of chromium allergy by cellular in vitro methods

“…Several previous studies used the LTT method to discriminate either between chromium-allergic or sensitized volunteers and healthy controls [13][14][15][16]. However, most of the studies were small (n = 8, 8, 5, and 31 allergic/ sensitized individuals, respectively) and none of them addressed the question as to whether sensitized patients with and without clinically manifest allergy were different in terms of their cellular in vitro reactions.…”

“…In contrast to previous studies that compared either allergic [13][14][15] or patch test-positive volunteers [16] with healthy controls, we considered three different groups: with sensitization with allergy, with sensitization without allergy, and non-sensitized healthy controls. We wanted to know whether cellular in vitro responses could discriminate between sensitized individuals with and without clinically manifest allergy.…”

Lymphocyte proliferation testing in chromium allergic contact dermatitis

“…In this study we investigated a number of LPT parameters to optimize testing for the diagnosis of chromium ACD. Once responses with various chromium salts, concentrations and incubation periods were obtained, we focused on these parameters in a preliminary study to investigate the best conditions in which to perform chromium LPT 14,16–20 . Initially, we chose a broad range of concentrations of chromium chloride [Cr(III)] and potassium dichromate [Cr(VI)] to be tested with both short and long incubation periods.…”

Estudo da proliferação linfocitária em pacientes sensibilizados ao níquel

“…4,5 Another diagnostic method mentioned in the literature but with varied and controversial results is the lymphocyte proliferation test -LPT. [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] Peripheral blood lymphocytes of patients are placed in contact with nickel in LPT. If the patient is sensitive to nickel, the circulating memory lymphocytes are activated, synthesize DNA and split.…”

“…6,7,9 Lymphocyte proliferative response was unspecific in cases and normal in controls, that is, nickel acted as a mitogen, stimulating the lymphocytes of sensitive patients as well as of non-sensitive controls. 6,9 Later, most studies were performed with nickel sulfate, 8,10,11,[13][14][15][16][17][18][19][20][21][22][23] but there is no standardization in the literature about cell and nickel concentration used.…”