Lymphocyte proliferation testing in chromium allergic contact dermatitis

Martins, Luis Eduardo Agner Machado; Duarte, Andressa; Aoki, Valéria; Nunes, Ricardo; Ogusuku, S.; Reis, Vítor Manoel Silva dos

doi:10.1111/j.1365-2230.2008.02725.x

Cited by 16 publications

(16 citation statements)

References 23 publications

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“…Occupationally-acquired dermal sensitization and allergic contact dermatitis from metals present opportunities for use of the LPT in better understanding underlying pathophysiologic mechanisms and in providing accurate and safer diagnostic methods.Martins and colleagues recently studied optimal conditions for performance of chromium LPT to detect allergy [42]. Those with chromium allergy, defined as dermatitis and positive patch tests, were compared to those without Cr dermatitis and negative patch tests.…”

Section: Review Of Lpt Uses To Assess Biologic Responses To Various Amentioning

confidence: 99%

The role of lymphocyte proliferation tests in assessing occupational sensitization and disease

Hines

Pacheco

Maier

2012

Current Opinion in Allergy &Amp; Clinical Immunology

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Purpose of Review Lymphocyte proliferation testing (LPT) is used in diagnosing occupationally-acquired delayed-type hypersensitivity. It has been used in beryllium-health effects, and it role is expanding in metal allergy. It may find application in diagnosis of other sensitizers. Recent findings Use of the beryllium LPT (BeLPT) in medical surveillance identifies beryllium sensitization at low exposure with chronic beryllium disease (CBD) that leads to physiologic impairment and need for immunosuppressive medications. New studies indicate that both beryllium exposure and genetic variation are associated with increased risk of CBD. Borderline positive BeLPTs warrant inclusion into diagnostic algorithms. Furthermore, use of LPTs to diagnose metal allergy is being proposed in diagnosis of chromium allergy and hypersensitivity to surgical implants. New occupational sensitizers continue to be identified including metalworking fluids, the sterilizing agent ortho-phthalaldehyde and the solvent parachlorobenzotrifluoride. Use of LPT in occupational surveillance to these agents, and other known sensitizers may play expanding roles. Summary Lymphocyte proliferation testing serves a valuable role in diagnosing occupational sensitization, as demonstrated with beryllium-health effects, as cases continue to be found at low exposure levels. The use of LPTs in diagnosing contact allergy is expanding, and new applications may be identified in human and animal studies.

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Section: Review Of Lpt Uses To Assess Biologic Responses To Various Amentioning

confidence: 99%

The role of lymphocyte proliferation tests in assessing occupational sensitization and disease

Hines

Pacheco

Maier

2012

Current Opinion in Allergy &Amp; Clinical Immunology

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“…This method consists in exposing the patient's lymphocytes to the suspected allergen. 3,13 Based on the new concepts of the pathogenesis of ACD, we should detect differences between the lymphocytes of sensitized and non-sensitized patients, since the inflammation in ACD results from the action of previously sensitized lymphocytes and of memory, effector or regulatory lymphocytes, all of them with a selective production of cytokines. 3,13 Among these differences, there is increased number of these cells (blastic transformation), cytokine proliferation and production; all of these changes can be measured.…”

Section: Introductionmentioning

confidence: 99%

“…3,13 Based on the new concepts of the pathogenesis of ACD, we should detect differences between the lymphocytes of sensitized and non-sensitized patients, since the inflammation in ACD results from the action of previously sensitized lymphocytes and of memory, effector or regulatory lymphocytes, all of them with a selective production of cytokines. 3,13 Among these differences, there is increased number of these cells (blastic transformation), cytokine proliferation and production; all of these changes can be measured. 3,13,14 No matter what the method is, the cells are cultivated in the presence and in the absence of the antigen, and the results found in the stimulated and unstimulated cultures are compared.…”

Section: Introductionmentioning

confidence: 99%

“…3,13 Among these differences, there is increased number of these cells (blastic transformation), cytokine proliferation and production; all of these changes can be measured. 3,13,14 No matter what the method is, the cells are cultivated in the presence and in the absence of the antigen, and the results found in the stimulated and unstimulated cultures are compared. 3,13,14 …”

Section: Introductionmentioning

confidence: 99%

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Cytokine detection for the diagnosis of chromium allergy

Martins

Reis

2013

An. Bras. Dermatol.

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BACKGROUNDPatch testing remains the gold standard method for the identification of the etiologic agent of allergic contact dermatitis. However, it is a subjective, time-consuming exam whose technique demands special care and which presents some contraindications, which hamper its use. In a recent study, we showed that the proliferation assay can suitably replace patch testing for the diagnosis of chromium allergy, which had been previously demonstrated only for nickel allergy. In this study, we try to refine the method by reducing the incubation period of cultures for lymphocyte proliferation assays in response to chromium. OBJECTIVEDevelop an alternative or complementary diagnostic test for chromium allergic contact dermatitis.METHODSWe compared the production of 9 cytokines (IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17 and RANTES) between 18 chromium-allergic patients and 19 controls. RESULTSChromium increased the production of IFN-y, IL-5, IL-2 and IL-13 in allergic patients, but only IL-2 and especially IL-13 helped discriminate allergic patients from controls. The sensitivity, specificity and accuracy found with IL-13 were about 80%. CONCLUSIONS IL-13 and IL-2 detection may be used to diagnose chromium allergy in 2-day cultures. However, in general, the 6-day cultures seem to be superior for this purpose.

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“…Furthermore, this procedure carries the risks of patient sensitization to new antigens and excited skin syndrome, and specialized training is necessary to interpret the results. Therefore, the lymphocyte transformation test (LTT) has attracted attention as a potential new option in testing for metal allergy 12,17) . However, accurate interpretation is difficult, as the LTT in humans can result in nonspecific lymphocyte proliferation and false negative results 6,10,15) .…”

Section: Introductionmentioning

confidence: 99%

Th1/Th2 Balance in Mouse Delayed-type Hypersensitivity Model with Mercuric Chloride via Skin and Oral Mucosa

Ukichi

Okamura

Fukushima

et al. 2011

Bull. Tokyo Dent. Coll.

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In order to compare delayed-type hypersensitivity (DTH) among different exposure sites, we evaluated the sensitization potency of mercuric chloride (HgCl 2 ) via exposure to the skin, or oral or esophageal mucosa using the mouse ear swelling test. Furthermore, we investigated in vitro splenocyte proliferation reaction and cytokine profile in HgCl 2 -exposed and control mice. Sensitization with HgCl 2 was established via the skin and oral mucosa but not via the esophageal mucosa. The splenocyte proliferation reaction was significantly enhanced to a similar degree in skin and oral mucosa-sensitized mice compared with in the control mice. IL-10 levels from cultured splenocytes were significantly increased in skin and oral mucosa-sensitized mice compared with those in control mice, whilst IFN-␥ significantly increased only in splenocytes from skin-sensitized mice. These results suggest that exposure of the skin or oral mucosa to HgCl 2 can induce DTH, but that Th1/Th2 balance differs according to the site of antigen exposure.

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Lymphocyte proliferation testing in chromium allergic contact dermatitis

Abstract: Further investigation is necessary to achieve better sensitivity values.

Cited by 16 publications

References 23 publications

The role of lymphocyte proliferation tests in assessing occupational sensitization and disease

The role of lymphocyte proliferation tests in assessing occupational sensitization and disease

Cytokine detection for the diagnosis of chromium allergy

Th1/Th2 Balance in Mouse Delayed-type Hypersensitivity Model with Mercuric Chloride via Skin and Oral Mucosa

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