In vitro intestinal epithelium responses to titanium dioxide nanoparticles

Pedata, Paola; Ricci, Giulia; Malorni, Livia; Venezia, Antonella; Cammarota, Marcella; Volpe, Maria Grazia; Iannaccone, Nunzia; Guida, Vincenzo; Schiraldi, Chiara; Romano, Marco; Iacomino, Giuseppe

doi:10.1016/j.foodres.2018.10.041

Cited by 48 publications

(36 citation statements)

References 52 publications

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“…Consequently, anatase influences the mitochondrial membrane and is more prone to activate the apoptosis pathway. TiO 2 NPs treated Caco-2 cell indicated the affected intestinal epithelium layer after 24 h exposure and the cell viability shows the 13% reduction compared to the control sample ( Pedata et al, 2019 ). Transmission electron microscope (TEM) observations showed that TiO 2 NPs were selectively accumulated in Caco-2 monolayers, as indicated in Figure 5 .…”

Section: Toxicity Of Common Metallic and Metal Oxide Npsmentioning

confidence: 99%

“…Transmission electron microscope (TEM) observations showed that TiO 2 NPs were selectively accumulated in Caco-2 monolayers, as indicated in Figure 5 . Also, it was proved that titanium ions have the potential to trigger the production of the pro-inflammatory cytokines and led to some toxic effects on the intestinal epithelium layer ( Pedata et al, 2019 ). Experiments on the A549 cell line (human lung epithelial cells) confirmed the significant cytotoxic effects of citrate-coated TiO 2 NPs also the DNA damage experiments by comet assay indicated the increasing genotoxic effects in these citrate coated NPs.…”

Section: Toxicity Of Common Metallic and Metal Oxide Npsmentioning

confidence: 99%

“…A peak at 25 eV compatible with the TiM 2,3 edge electron energy loss was detected. Adapted from Pedata et al (2019) with permission from Elsevier.…”

Section: Toxicity Of Common Metallic and Metal Oxide Npsmentioning

confidence: 99%

See 2 more Smart Citations

The Toxicity Phenomenon and the Related Occurrence in Metal and Metal Oxide Nanoparticles: A Brief Review From the Biomedical Perspective

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Front. Bioeng. Biotechnol.

158

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Thousands of different nanoparticles (NPs) involve in our daily life with various origins from food, cosmetics, drugs, etc. It is believed that decreasing the size of materials up to nanometer levels can facilitate their unfavorable absorption since they can pass the natural barriers of live tissues and organs even, they can go across the relatively impermeable membranes. The interaction of these NPs with the biological environment disturbs the natural functions of cells and its components and cause health issues. In the lack of the detailed and comprehensive standard protocols about the toxicity of NPs materials, their control, and effects, this review study focuses on the current research literature about the related factors in toxicity of NPs such as size, concentration, etc. with an emphasis on metal and metal oxide nanoparticles. The goal of the study is to highlight their potential hazard and the advancement of green non-cytotoxic nanomaterials with safe threshold dose levels to resolve the toxicity issues. This study supports the NPs design along with minimizing the adverse effects of nanoparticles especially those used in biological treatments.

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Section: Toxicity Of Common Metallic and Metal Oxide Npsmentioning

confidence: 99%

Section: Toxicity Of Common Metallic and Metal Oxide Npsmentioning

confidence: 99%

See 1 more Smart Citation

The Toxicity Phenomenon and the Related Occurrence in Metal and Metal Oxide Nanoparticles: A Brief Review From the Biomedical Perspective

Attarilar

Yang

Ebrahimi

et al. 2020

Front. Bioeng. Biotechnol.

158

View full text Add to dashboard Cite

show abstract

“…A few recent experiments on a well-established cell line Caco-2 support former studies, indicating detrimental effects on the intestinal epithelium layer. Taken together, the results point out that exposure to TiO 2 NPs has the following local effects: induces an inflammatory response [ 69 , 70 , 71 , 72 ]; increases the release of mucins and the expression of some efflux pumps [ 69 ]; increases ROS generation [ 70 ]; induces morphological changes or decreases the number of intestinal microvilli, which in turn decreases the surface area needed for optimal nutrients absorption [ 70 , 72 , 73 ]; leads to their internalization and entrapment by Caco-2 monolayers [ 71 ]. …”

Section: Routes Of Exposure and Toxicity Of Tio 2 mentioning

confidence: 89%

Titanium Dioxide Nanoparticles in Food and Personal Care Products—What Do We Know about Their Safety?

et al. 2020

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Titanium dioxide (TiO2) is a material of diverse applications commonly used as a food additive or cosmetic ingredient. Its prevalence in products of everyday use, especially in nanosize, raises concerns about safety. Current findings on the safety of titanium dioxide nanoparticles (TiO2 NPs) used as a food additive or a sunscreen compound are reviewed and systematized in this publication. Although some studies state that TiO2 NPs are not harmful to humans through ingestion or via dermal exposure, there is a considerable number of data that demonstrated their toxic effects in animal models. The final agreement on the safety of this nanomaterial has not yet been reached among researchers. There is also a lack of official, standardized guidelines for thorough characterization of TiO2 NPs in food and cosmetic products, provided by international authorities. Recent advances in the application of ‘green-synthesized’ TiO2 NPs, as well as comparative studies of the properties of ‘biogenic’ and ‘traditional’ nanoparticles, are presented. To conclude, perspectives and directions for further studies on the toxicity of TiO2 NPs are proposed.

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“…The exact mechanism of action is still unknown. A possible mechanism is induction of oxidative stress, which results in the formation of reactive oxygen species (ROS) (Ranjan et al, 2016b ; Fadda et al, 2018 ; Huerta-García et al, 2018 ; Gea et al, 2019 ; Pedata et al, 2019 ; Santonastaso et al, 2019 ). As such, any possible adverse effects of ingested TNPs need to be examined to rule out any health risk associated with consumption of TNP-containing products.…”

Section: Introductionmentioning

confidence: 99%

Involvement of Bcl-2 Activation and G1 Cell Cycle Arrest in Colon Cancer Cells Induced by Titanium Dioxide Nanoparticles Synthesized by Microwave-Assisted Hybrid Approach

Ranjan

Dasgupta

Mishra

et al. 2020

Front. Bioeng. Biotechnol.

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The toxic effect of TiO 2 nanoparticles (TNP) greatly varies with the variation in synthesis protocol followed. Any morphological alteration of TNPs affects their activity. In the present study, we report the detailed toxicological analysis of TNPs fabricated by a microwave irradiation-assisted hybrid chemical approach. The toxicological mechanism was studied in human colon cancer cell lines (HCT116). Results indicate that TNP induces oxidative stress on HCT116, which, in turn, causes mitochondrial membrane depolarization. We also observed activation of Bcl-2 and caspase-3 by Western blot analysis. This indicates TNPs induce mitochondrial-mediated apoptosis. Furthermore, G1 cell cycle arrest was observed by flow-cytometric analysis. This study provides an understanding of the mechanism of action for apoptosis induced by TNPs, which can be further used to design safe TNPs for various consumer products and also suggests that extensive research needs to be done on harmful effects of TNPs synthesized from different approaches before commercial application.

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In vitro intestinal epithelium responses to titanium dioxide nanoparticles

Cited by 48 publications

References 52 publications

The Toxicity Phenomenon and the Related Occurrence in Metal and Metal Oxide Nanoparticles: A Brief Review From the Biomedical Perspective

The Toxicity Phenomenon and the Related Occurrence in Metal and Metal Oxide Nanoparticles: A Brief Review From the Biomedical Perspective

Titanium Dioxide Nanoparticles in Food and Personal Care Products—What Do We Know about Their Safety?

Involvement of Bcl-2 Activation and G1 Cell Cycle Arrest in Colon Cancer Cells Induced by Titanium Dioxide Nanoparticles Synthesized by Microwave-Assisted Hybrid Approach

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