In-vitro interaction of cerium oxide nanoparticles with hemoglobin, insulin, and dsDNA at 310.15 K: Physicochemical, spectroscopic and in-silico study

Chetty, Rajlakshmi; Singh, Man

doi:10.1016/j.ijbiomac.2020.03.067

Cited by 17 publications

(16 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 Oxidation potentials and numbers of electronegative oxygen atoms are related to their ability to interact with biomacromolecules, e.g., proteins, nucleic acids, and others in specic medium. 3 Cerium(IV) oxide is also known as cerium dioxide, ceria oxide, ceria dioxide and ceria, and has been widely used as an antibacterial agent, biosensor, catalyst, uorescent material, and for agricultural applications. [4][5][6][7] Nanoceria is a cerium oxide with a size between 1 and 100 nm, and has diverse applications such as sensors and drug delivery 8,9 due to its attractive properties, e.g., biocompatibility, chemical and thermal stability,…”

mentioning

confidence: 99%

Synthesis and characterization of nanoceria for electrochemical sensing applications

et al. 2021

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Synthesis and characterization of nanoceria for electrochemical sensing applications

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The implication of this statement is that electrostatic interaction is purely physical in nature which may not describe quantitatively an equilibrium state of a system. The domain of steric interaction occurs at Thymidine 12 (DT) moiety (the macromolecule to which the moiety belongs was not explicitly stated by the authors) [23]. The electrostatic interaction which was reported to have taken place between C2 of DT 12 (perhaps thymidine 12 of ds-DNA) and Ce atom and C6 of DT 12 and O atom of CNP at 2.466 and 2.312 Å may be the highest with the highest energy.…”

Section: Non-equilibrium Binding and Total Translational Energiesmentioning

confidence: 98%

“…(A.34)) is the total electrostatic force of attraction derived in the appendix section and q the square of the instantaneous translational velocity is given as  2 (Eq. (1)) /m 2 ;  is the viscosity coefficient equal to 0.697 kg/m s at 310.15 k [23] and R E is the hydrodynamic radius of the enzyme. Substitution of Eq.…”

Section: Review Of Theorymentioning

confidence: 99%

“…The binding of cerium oxide nanoparticles, CNP to ds-deoxyribonucleic acid, ds-DNA occur by a nonbonding mode, an electrostatic interaction [23]. The implication of this statement is that electrostatic interaction is purely physical in nature which may not describe quantitatively an equilibrium state of a system.…”

Section: Non-equilibrium Binding and Total Translational Energiesmentioning

confidence: 99%

“…The importance of non-equilibrium binding energies is better corroborated or elucidated with examples of researches where binding affinity is regularly mentioned apart from the formation of encounter complex formation preceding enzyme-substrate complex formation used as model in this research. The webderimers (poly(amidoamine)) that are believed to possess binding affinity for nanoparticles for drug and gene encapsulation for drug transport and delivery [42] and cerium oxide nanoparticles (CNPs) for therapeutic applications for the cure of neurological oxidative stresses [23] requires information about the binding energy with both target and unintended target organ, tissue, cell etc. The 1st tier dendrimers with a common 1, 3,5-benzenetricarbonyl trichloride/trimesoyl chloride (TMC) core [42] can only exhibit its scavenging activity against reactive oxygen species (free radicals which triggers carcinogenesis, cardiovascular disease etc) in an effective manner if they possess strong binding affinity (energy) that remains essentially irreversible.…”

Section: Importance Of Non-equilibrium Binding Energymentioning

confidence: 99%

See 2 more Smart Citations

Non-equilibrium Binding Energy Determined Using Alpha-amylase Catalysed Amylolysis of Gelatinised Starch as a Probable Generalisable Model and Importance

Udema¹

2020

AJOCS

View full text Add to dashboard Cite

Objectives: This research was undertaken to determine the non–equilibrium binding energy by calculation after substituting experimental data into derived equations, present its role distinct from energy associated with activated enzyme–substrate (ES) complex and ultimately elucidate the importance of binding energies. Background: There are overwhelming pieces of evidence in the literature that binding interaction is essential for the ultimate transformation of a substrate, inhibition of vital enzymes of pathogens, covid-19 in particular. Intrinsic binding energy herein referred to as non–equilibrium binding energy and energy associated with activated ES are seen to be chemical in origin. Much attention seemed not to be given to theoretical approach to the determination of non–equilibrium binding energy. Methods: Experimental approach (Bernfeld method of enzyme assay) and calculational. Results and Discussion: The non–equilibrium translational (2.691–2.726 kJ/mol) and total electrostatic energies (2.755-3.154 kJ/mol) were > than the thermal energy at 310.15 k. The interfacial distance between the bullet and target molecule was expectedly very short; the range was between 6.672 and 7.570 exp (- 12) m. This was attributed to the interaction between charged enzyme and weakly polar substrate. Conclusion: The equations of non–equilibrium and translational energies were derivable. The binding interaction serves to fix the bullet molecule on or into the target (supra) molecule before the commencement of transition state formation. The non–equilibrium binding interactions of the bullet (drugs, substrate, etc) and target (receptors e.g. enzymes, pathogens such as Covid–19, Plasmodium etc) and the ultimate complex are likely to be stabilised against the thermal energy in furtherance of enzymatic and drug action since the electrostatic interaction energy is higher than thermal energy.

show abstract

Green Synthesis, in vitro Cytotoxicity, Antioxidant Activity and Interaction Studies of CuO Nanoparticles with DNA, Serum Albumin, Hemoglobin and Lysozyme

2022

View full text Add to dashboard Cite

The present study reports an environmentally safe synthesis of CuO NPs employing Echinophora platyloba DC extract. The production of CuO NPs was authenticated using various analytical characterization techniques such as FT-IR, TEM, XRD, SEM-EDX, DLS, zeta potential, and UV-Vis. The DPPH assay was employed to assess the scavenging efficacy of synthesized CuO NPs. The results of the MTT assay exhibited that CuO NPs have efficient anticancer potential on two used cancer cell lines (MCF-7 and Burkitt's lymphoma). Thus, it tempts us to study the synthesized CuO NPs biocompatible aspects by interactions with several biologically significant molecules. The present experimental evidence demonstrates the direct interaction of CuO NPs with DNA, which might be one of the credible highways elevating its anticancer potential. Moreover, we investigated the interaction of CuO NPs with the most important blood proteins (HSA, HHb, and Lys) in simulated physiological conditions using spectroscopic methods. The interaction findings suggest that the native conformation of the above proteins was preserved at the level of secondary structure in spite of complex formations with CuO NPs. The binding constant values revealed the greater binding affinity of CuO NPs with HSA, HHb, and Lys as compared to ct-DNA; the binding affinity was found in the order HSA > HHb > Lys > ct-DNA.

show abstract

In-vitro interaction of cerium oxide nanoparticles with hemoglobin, insulin, and dsDNA at 310.15 K: Physicochemical, spectroscopic and in-silico study

Cited by 17 publications

References 70 publications

Synthesis and characterization of nanoceria for electrochemical sensing applications

Synthesis and characterization of nanoceria for electrochemical sensing applications

Non-equilibrium Binding Energy Determined Using Alpha-amylase Catalysed Amylolysis of Gelatinised Starch as a Probable Generalisable Model and Importance

Green Synthesis, in vitro Cytotoxicity, Antioxidant Activity and Interaction Studies of CuO Nanoparticles with DNA, Serum Albumin, Hemoglobin and Lysozyme

Contact Info

Product

Resources

About