In vitro inoculation of Aeromonas hydrophila and Pseudomonas fluorescens in cryopreserved silver barb (Barbodes gonionotus) milt: Effect on fertilization capacity and transmission potential to embryos

Boonthai, Traimat; Khaopong, Weerasith; Sangsong, Jumlong; Vuthiphandchai, Verapong; Nimrat, Subuntith

doi:10.1016/j.theriogenology.2017.11.009

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…Similarly, in vitro insemination of cryopreserved sperm showed that bacteria present in cryopreserved sperm affected the fertilization capacity of sperm and was responsible for the bacterial transmission to embryos. 25 These results suggest the importance of revealing sperm microbiota composition and its relationship with sperm quality, as there is still a paucity of investigations on sperm microbiota. Boonthai et al 21 indicated that appropriate sperm collection methods could reduce sperm-related bacterial abundance; however, there was no significant difference in sperm quality.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

Koh

Nizam

Abduh

et al. 2019

Evol Bioinform Online

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Malaysian Mahseer ( Tor tambroides ) is considered as a good prospect for aquaculture in Malaysia. However, knowledge about Malaysian Mahseer-associated sperm microbiota is still limited, although some studies reported that sperm-related bacteria are a factor in the decline of sperm quality, as sperm may become the carrier of pathogenic bacteria to the egg. The goal of this study was to evaluate the sperm microbiota associated with Malaysian Mahseer from 3 different locations (Universiti Malaysia Terengganu [UMT], Ajil, and Pahang) using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting and to compare location differences by cluster analysis. Our results showed that the UMT sample had different sperm microbiota composition and a different trend in its relationship with sperm quality. Correlation analysis showed a relationship between bacterial diversity and sperm quality. Phylogenetic analysis indicated that sperm microbiota was composed of diverse phyla, including Proteobacteria, Firmicutes, and Actinobacteria. Interestingly, bacteria such as Salinisphaera sp., Pelomonas sp., and Staphylococcus spp. were detected in all the locations, suggesting that these bacteria are indigenous bacterial members of the Malaysian Mahseer sperm microbiota, although their function is still unclear.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

Koh

Nizam

Abduh

et al. 2019

Evol Bioinform Online

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“…The bacteria is usually transmitted after insemination of the fish eggs with tainted cryopreserved sperm, which leads to reduced fertilization capacity as the embryos die and egg production gets disrupted. (Boonthai et al, 2016;Boonthai et al, 2018).…”

Section: Aeromonasmentioning

confidence: 99%

Effect of Dietary Papaya Peel and Baker’s Yeast Supplementation on Growth Performance, Hematological Value of Silver Barb (Barbonymus Gonionotus) and Its Resistance to Aeromonas Hydrophila

Phinyo¹,

Areeya²,

Wangkahart³

2021

J. Sustain. Sci. Manage.

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“…The storage of the sperm of some fish is enhanced with antibiotics and antioxidants. The success of short-term sperm storage is based on motility and cell viability and is hampered by infection or bacterial development in the sperm volume and/or sperm solution [11]. This reduces fertilizing potential, cell quality, and viability, endangering germplasm [12].…”

Section: Introductionmentioning

confidence: 99%

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

et al. 2023

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Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of >43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries.

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In vitro inoculation of Aeromonas hydrophila and Pseudomonas fluorescens in cryopreserved silver barb (Barbodes gonionotus) milt: Effect on fertilization capacity and transmission potential to embryos

Cited by 9 publications

References 28 publications

Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

Effect of Dietary Papaya Peel and Baker’s Yeast Supplementation on Growth Performance, Hematological Value of Silver Barb (Barbonymus Gonionotus) and Its Resistance to Aeromonas Hydrophila

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

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