In vitro Inhibitory Effects of Disodium Cromoglycate on Ionic Transports Involved in Sickle Cell Dehydration

Bizumukama, Léonidas; Ferster, Alina; Gulbis, Béatrice; Kumps, Alain; Cotton, Frédéric

doi:10.1159/000215598

Cited by 3 publications

(2 citation statements)

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“…The bioactivity was assessed based on the phenotypic sickling of SS red blood cells (RBCs) in the presence of an oxygenscavenging agent. The individual plant extracts and DEE displayed remarkable sickling inhibitory effects, reverting sickle erythrocytes to a typically normal morphology in the same proportions as cromoglycate, a well-known positive control (18,24,30). It confirms that the DEE extract conserves the previously shown antisickling effects of Moringa oleifera extracts 24 and Drepanoalpha ® herbal powder.…”

Section: Resultssupporting

confidence: 70%

In Vitro Biological Activities of Drepanoalpha® Ethanolic Extract, A Justicia Secunda and Moringa Oleifera-Based Phytomedicine Proposed for The Symptomatic Treatment of Sickle Cell Disease

Zoawe

Nachtergael

Tshibangu

et al. 2023

JFAPS

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Sickle cell disease (SCD) is an autosomal recessive blood disorder characterized by red blood cells that assume an abnormal, rigid sickle shape under low-oxygen conditions. These sickle-shaped erythrocytes tend to lyse, aggregate, and obstruct small blood vessels, leading to major complications. The present study aims to investigate properties that may underlie the activity of Drepanoalphaâ, an antisickling herbal formulation developed in the Democratic Republic of Congo (DRC) for the prevention and symptomatic treatment of sickle cell disease crises. The Drepanoalpha® Ethanolic Extract (DEE) is a dry extract (drug-extract ratio, DER, 100/11) prepared from ethanol (96 %, v/v) percolation of a 1:1 mixture of 2 food plants, Justicia secunda Vahl and Moringa oleifera Lam. Sickling was classically measured by light microscopy on diluted washed erythrocytes obtained from homozygote patients; erythrocytes were treated with 2 % Na2S2O5 in the presence of DEE (suspension in 9 ‰ NaCl), 9 ‰ NaCl (negative control) or disodium cromoglycate (DSCG, positive control). For all tested conditions, the sickle hemoglobin polymerization, the Fe2+/Fe3+ ratio, and the median corpuscular fragility were measured by spectrophotometry. The DEE reversed sickling by 89.1 %, comparable to DSCG (87.7 %; 60.3 µg/mL), inhibiting sickle cell hemoglobin polymerization of 77.8 % and 74.4 %, respectively. The Fe2+/Fe3+ ratio was improved by 18.0 % for DEE and 15.9 % for DSCG. The median corpuscular fragility values were 0.602, 0.714, and 0.732 for NaCl 9 ‰, DSCG, and DEE, respectively. The measured in vitro parameters validate an effective antisickling effect of DEE and confirm the value of this improved traditional herbal formulation for the management of SCD.

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Section: Resultssupporting

confidence: 70%

In Vitro Biological Activities of Drepanoalpha® Ethanolic Extract, A Justicia Secunda and Moringa Oleifera-Based Phytomedicine Proposed for The Symptomatic Treatment of Sickle Cell Disease

Zoawe

Nachtergael

Tshibangu

et al. 2023

JFAPS

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“…Cependant, il a été constaté que les flavonoïdes totaux extraits du Drepanoalpha® montrent une forte inhibition de la falciformation au même titre que le cromoglycate de sodium utilisé comme contrôle positif. La comparaison de ces différentes valeurs avec celle du NaCl 0,9 % prouvent que le NaCl 0,9 % ne contient aucun principe actif capable d'inhiber la formation des drépanocytes alors que flavonoïdes totaux possèdent une activité antifalcémiante prouvée et que le cromoglycate de sodium possède une molécule active bien connue (16,19). La falciformation des globules rouges est un processus qui résulte de la polymérisation de l'Hb S dans des conditions d'hypoxie et de déshydratation cellulaire par perte d'ions (K + , Cl -, Mg ++ ) et d'eau (20)(21).…”

Section: Caractéristiques Microscopiques De La Poudre Du Matériel Vég...unclassified

Profilage phytochimique par chromatographie sur couche mince haute performance d’extraits de flavonoïdes totaux du Drepanoalpha® et évaluation de leur activité anti-drépanocytaire in vitro

Gbolo¹,

Ciala²,

Ngbolua³

et al. 2022

Ann. Afr. Med

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Contexte et objectif. Le défi le plus important dans la drépanocytose consiste à améliorer l’état de santé des patients dans les pays en développement. L'une des meilleures solutions est donc le développement de la phytomédecine basée sur la connaissance de la pharmacopée traditionnelle. L’objectif de la présente étude était d’évaluer les activités anti-drépanocytaires des flavonoïdes totaux extraits du phytomédicament Drépanoalpha® d’une part et déterminer leur profilage chimique par chromatographie sur couche mince haute performance d’autre part. Méthodes. Les flavonoïdes totaux ont été obtenus par fractionnement de l’extrait méthanolique par chromatographie flash (PURIFLASH COLUMN 30 SILICA HP - 12,0 g) et purifies à l’aide d’une cartouche (Polymeric Reversed Phase) puis caractérisés et dosés par chromatographie sur couche mince haute performance (CCMHP). L’activité anti-drépanocytaire a été mise en évidence grâce aux tests d’Emmel, de polymérisation, de rapport Fe2+/Fe3+, d’hémolyse et de la fragilité osmotique membranaire. Résultats. La poudre du Drépanoalpha® contenait une quantité de flavonoïdes totaux de 8,14 mg équivalent de quercétine/g d'extrait. Les flavonoïdes totaux extraits du Drépanoalpha® possèdent une activité antifalcémiante (avec le taux maximal de normalisation d’environ 90 % et une concentration minimale de normalisations de 11,4 μg/mL), un taux d’augmentation du rapport Fe2+/Fe3+ de 97,0 %, une activité anti-hémolytique avec une fragilité corpusculaire membranaire des érythrocytes (FCM) de 0,73 et un taux d’inhibition de la polymérisation de 77,5%. Conclusion. La pertinence des résultats de cette étude permet de confirmer les flavonoids comme phytomarqueur pour le contrôle de qualité et de standardisation de cet alicament. English Title: In vitro evaluation of anti-sickling activity of crude flavonoid extracted from Drepanoalpha® and their phytochemical profiling by high performance thin layer chromatography Abstract Context and objective. The most important challenge in Sickle cell disease is to improve the health status of patients in developing countries. One of the best solutions is the development of phytomedicine based on the knowledge of the traditional pharmacopoeia. This study aimed to evaluate the anti-sickling activities of crude flavonoids extracted from the phytomedicine Drépanoalpha® on the one hand and to determine their chemical profiling by high performance thin layer chromatography on the other hand. Methods. Crude flavonoids were obtained by fractionation of the methanolic extract by flash chromatography (PURIFLASH COLUMN 30 SILICA HP - 12.0 g) and purified using a cartridge (Polymeric Reversed Phase) then characterized and assayed by high performance thin layer chromatography (HPTLC). The anti-sickling activity was carried out using Emmel, polymerization, Fe2+/Fe3+ ratio, hemolysis and membrane osmotic fragility tests. Results. Drepanoalpha® powder contained an amount of total flavonoids of 8.14 mg quercetin eq/g extract. The total flavonoids extracted from Drépanoalpha® have an antisickling activity (with a maximum reversibility rate of about 90% and a minimum reversibility concentration of 11.4 μg/mL), an increase rate of the Fe2+/Fe3+ ratio of 97.0%, an antihemolytic activity with a corpuscular membrane fragility of erythrocytes (FCM) of 0.73 and a polymerization inhibition rate of 77.5%. Conclusion. Findings from the present study confirm flavonoids as a phytomarker for the quality control and standardization of Drépanoalpha®. Keywords: Drépanoalpha®, Sickle cell disease, Hemoglobin S, Polymerization, Methemoglobin, Flavonoids

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Effects of disodium cromoglycate on cationic exchange of deoxygenated sickle cells

Bizumukama¹,

Ferster²,

Gulbis³

et al. 2011

European Journal of Pharmacology

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In vitro Inhibitory Effects of Disodium Cromoglycate on Ionic Transports Involved in Sickle Cell Dehydration

Cited by 3 publications

References 69 publications

In Vitro Biological Activities of Drepanoalpha® Ethanolic Extract, A Justicia Secunda and Moringa Oleifera-Based Phytomedicine Proposed for The Symptomatic Treatment of Sickle Cell Disease

In Vitro Biological Activities of Drepanoalpha® Ethanolic Extract, A Justicia Secunda and Moringa Oleifera-Based Phytomedicine Proposed for The Symptomatic Treatment of Sickle Cell Disease

Profilage phytochimique par chromatographie sur couche mince haute performance d’extraits de flavonoïdes totaux du Drepanoalpha® et évaluation de leur activité anti-drépanocytaire in vitro

Effects of disodium cromoglycate on cationic exchange of deoxygenated sickle cells

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