In vitro induction and characterization of tetraploid Patchouli (Pogostemon cablin Benth.) plant

Widoretno, Wahyu

doi:10.1007/s11240-016-0946-0

Cited by 45 publications

(25 citation statements)

References 20 publications

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“…Pande and Khetmalas [22] also reported such growth inhibitory effects on Stevia rebaudiana using mutagenic sodium azide and colchicine at 0 to 0.250 % for 12 and 24 h at room temperature. The reduction in germination rates with subsequent negative effects on seedling growth were also reported by Widoretno [23] in Pogostemon cablin Benth and Khalili et al [24] in Gerbera jamesonii Bolus cv. Mini Red.…”

Section: Effect Of Colchicine On Seedling Phytochemicals and Nucleic Acidsupporting

confidence: 75%

Germination, Morphological and Physiological Evaluation of Seedlings Pretreated with Colchicine in Soybean (Glycine max L)

Mangena

2021

Walailak J Sci & Tech

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Wider genetic diversity has the potential to improve crop productivity of soybean, especially under environmental stress conditions. The pre-treatment of soybean seeds with antimitotic agents to establish improved genetic pool may also contribute to the enhancement of germination, seedling development, morpho-physiological growth and yield. In this study, 2 soybean genotypes viz. TGx1835-10E and Dundee were imbibed in solutions containing different amounts of colchicine (0.0, 0.1, 0.5 and 1 %) to evaluate the variations in germination, morphometric and physiological parameters. The seeds were imbibed for the period of 12 and 24 h before sowing for germination in plastic pots containing moistened sterile vermiculite. The variance components expressed as means, and mean percentage of total variations showed that colchicine concentration and imbibitional duration were the most important sources of variation for all traits, followed by the genotypes. Significant responses were detected for various germination parameters, seedling morphology and physiological contents such as; chlorophyll content, total phenolics, flavonoids as well as total protein and DNA content in the 2 genotypes used. HIGHLIGHTS Mutagenic pre-treatment of seeds via imbibition Germination and seedling growths of colchicine preconditioned seeds Chloroplastidic pigment analysis of pre-treated seedlings Physiological valuation of primary and secondary metabolites in grown seedlings Role of colchicine on germination, seedling development and growth of soybean plantlets GRAPHICAL ABSTRACT

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Section: Effect Of Colchicine On Seedling Phytochemicals and Nucleic Acidsupporting

confidence: 75%

Germination, Morphological and Physiological Evaluation of Seedlings Pretreated with Colchicine in Soybean (Glycine max L)

Mangena

2021

Walailak J Sci & Tech

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“…Although many studies provide polyploidization protocols (Rêgo et al, 2011;Gomes et al, 2014;Tavan et al, 2015;Widoretno, 2016;Salma et al, 2017;Denaeghe et al, 2018), they generally demonstrate that the optimal procedures are rather species specific. The development of a proper method for polyploidization requires the conduction of several tests to obtain the most suitable combination of antimitotic agent, concentration, exposure time, type of explants, exposure method, and the regeneration mode employed (Allum et al, 2007;Dhooghe et al, 2011;Yang et al, 2014;Sattler et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

et al. 2020

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Polyploidy is widely recognized as a major evolutionary force in plants and has been reported in the genus Lippia (Verbenaceae). Lippia alba, the most studied species, has been documented as a polyploid complex involving at least four ploidal levels. L. alba presents remarkable chemical and genetic variation and represents a model for understanding genome organization. Although the economic and medicinal importance of the species has been widely described, no established polyploid induction protocol has been reported so far. Here, we describe the production of synthetic polyploid plants of L. alba using colchicine. The ploidal levels were estimated by flow cytometry and chromosome counting. In addition, FISH and molecular markers approaches were used to confirm the stability of the synthetic polyploids. The major component of the essential oils was estimated by GCMS to compare with the natural individuals. Tetraploids and triploids were produced providing new opportunities for investigating medicinal, pharmacological, and economic applications as well as addressing intrinsic questions involved in the polyploidization process in tropical plants.

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“…( 2017),where, the Trachyspermum ammi plants exposure to colchicine caused a significant increase in the stomata length compared to the control plants. In the same way, Pogostemon cablin Benth tetraploid plants exhibited larger stomatal size than diploid plants (Widoretno 2016). Similarly, in a study conducted by Gallone et al (2014) in "Oratia Beauty" Hebe plants with different ploidy level, induced with colchicine (500 and 1000 μM) the stomata length was significantly higher in tetraploids compared to diploids.…”

Section: Stomatic Characteristicsmentioning

confidence: 64%

Impact of colchicine on leaf morphology and stomatics of Kalanchoe tubiflora (Harv.) Raym.-Hamet (Crassulaceae)

Mercado¹,

Caleño²,

Urbano³

2021

Bot Pac

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The demand of medicinal plants for consumption is greatly increasing worldwide. The conventional breeding programs are generally dependent on the environment prone to biotic and abiotic stresses. These added to the low content of secondary metabolites at harvest, bring the need for artificial development of polyploid individuals as an alternative to increase productivity. Consequently, the present study evaluated the effect of different colchicine concentrations and exposure time, on Kalanchoe tubiflora leaf morphology and stomata. Initially, K. tubiflora seedlings were harvested and submitted to colchicine concentrations of 0, 0.025, 0.05 and 0.1 % and at two exposure times (24 and 48 hours). Subsequently, morphological measurements such as plant height, leaf width, leaves number, leaf length, leaf thickness and leaf volume every 15 days were made for 16 weeks after planting. Then, the stomata were characterized, taking into account the width, length, stomatal index and the number of chloroplasts per stoma. A significant increase in leaf morphology was found in colchicine treatments of 0.025 % at 48 h and 0.1 % at 24 h. A significant increase in stomatal morphology with the treatment of 0.025 % at 24 h was also recorded. This shows that the correct application of colchicine in term of quantity and time could produce greater growth in a short period and increase the biomass of K. tubiflora medicinal plant.

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In vitro induction and characterization of tetraploid Patchouli (Pogostemon cablin Benth.) plant

Cited by 45 publications

References 20 publications

Germination, Morphological and Physiological Evaluation of Seedlings Pretreated with Colchicine in Soybean (Glycine max L)

Germination, Morphological and Physiological Evaluation of Seedlings Pretreated with Colchicine in Soybean (Glycine max L)

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

Impact of colchicine on leaf morphology and stomatics of Kalanchoe tubiflora (Harv.) Raym.-Hamet (Crassulaceae)

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