In vitro–in vivo characterization of gentamicin bone implants

Baro, Manuel; Sánchez, Esther; Delgado, Araceli; Perera, A.; Évora, Carmen

doi:10.1016/s0168-3659(02)00179-7

Cited by 102 publications

(66 citation statements)

References 28 publications

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“…On the protocol in which only half of the medium was replaced, concentration gradient was lower, explaining the slower CHX release kinetics over the 14 days incubation. The observed behavior is in agreement with several studies showing that controlled drug release profiles usually reveal an initial burst release of short duration that is followed by a longer period of continuous but declining release [4,13,18,[42][43][44], and that the release pattern is dependent on the amount of antimicrobial agent adsorbed on biomaterials [13,44].…”

Section: Discussionsupporting

confidence: 91%

“…CHX release results from desorption or partial dissolution of the CHX-phosphate binding. As expected, CHX release kinetics was clearly affected by the protocol used, because CHX is prone to leach, especially close to the discs' surface where rapid diffusion of water molecules was facilitated [13,4,42]. This process depends on the concentration gradient between the bulk solution and the disc surface.…”

Section: Discussionmentioning

confidence: 56%

“…There is a broad choice of active molecules such as anticoagulants, antiinflammatory drugs, antibiotics and extracellular matrix proteins [2]. Several authors [4][5][6][7][8] reported that the adsorption of antibiotics (e.g., gentamicin, ciprofloxacin, amoxicillin, and erythromycin) on medical devices has a huge potential in the medical field. How-ever, there is a consensus in the medical area on the rational use of antibiotics in order to avoid microbial resistance.…”

Section: Introductionmentioning

confidence: 99%

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Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Nanohydroxyapatite possesses exceptional biocompatibility and bioactivity regarding bone cells and tis-sues, justifying its use as a coating material or as a bone substitute. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. Surface functionalization with antimicrobials is a promising strategy to reduce the likelihood of bacterial infestation and colonization on medical devices. Chlorhexidine digluconate is a common and effective antimicrobial agent used for a wide range of medical applications. The purpose of this work was the development of a nanoHA biomaterial loaded with CHX to prevent surface bacterial accumulation and, simultaneously, with good cytocompatibility, for application in the medical field. CHX (5-1500 mg/L) was loaded onto nanoHA discs and the materials were evaluated for CHX adsorption and release profile, physic-chemical features, antibacterial activity against Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis, and cytocompatibility toward L929 fibroblasts. Results showed that the adsorption of CHX on nanoHA surface occurred by electrostatic inter-actions between the cationic group of CHX and the phosphate group of nanoHA. The release of CHX from CHX-loaded nanoHA showed a fast initial rate followed by a slower kinetics release, due to constraints caused by dilution and diffusion-limiting processes. NanoHA.50 to nanoHA.1500 showed strong anti-sessile activity, inhibiting bacterial adhesion and the biofilm formation. CHX-nanoHA caused a dose-and time-dependent inhibitory effect on the proliferation of fibroblasts for nanoHA.100 to nanoHA.1500. Cellular behavior on 2 nanoHA.5 and nanoHA.50 was similar to control. Therefore, CHX-loaded nanoHA surfaces appear as a promising alternative to prevention of devices-related infections.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

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Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…The absorbing properties of HAP have been exploited in the formation of HAP-antibiotic and HAP-drug composites for slow release in situ [14][15][16]. This has proved effective in the treatment of diseases such as osteomyelitis as it allows better recovery from the disease [17].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterisation of nanohydroxyapatite using an ultrasound assisted method

Poinern

Brundavanam

Mondinos

et al. 2009

Ultrasonics Sonochemistry

151

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Nanostructured hydroxyapatite (HAP) was prepared by a wet precipitation method using Ca(NO 3 ) and KH 2 PO 4 as the main material and NH 3 as the precipitator under ultrasonic irradiation. The Ca/P ratio was set at 1.67 and the pH maintained at a minimum of 9. The temperature conditions and ultrasound influences were investigated using X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM) and Fourier Transform Infrared Spectroscopy (FT-IR). The results showed that Nano-HAP can be obtained by this method and the particles were achieved to around 30nm.

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“…Besides the polymeric matrix, other controlled release systems based on different materials have been intensively studied in recent years, such as bioceramic and composites. Some examples are antibiotics like gentamicin, released from the matrix composed of calcium phosphates and poly(DL-lactide) (PLA) (Baro et al, 2002) and from the composite of hydroxyapatite/collagen (Martins & Goissis, 2000), and antineoplastic agents like cisplatin, released from calcium phosphate (CaP) nanoparticles (Barroug et al, 2004). The porosity of conventional matrices is highly heterogeneous due to complex chemical composition, which presents as a great disadvantage the difficulty of ensuring homogeneous drug distribution through the matrix, affecting the release rate.…”

Section: Controlled Release Of Drugsmentioning

confidence: 99%

Multifunctional Nanocomposites Based on Mesoporous Silica: Potential Applications in Biomedicine

Sousa¹,

Souza²,

Mohallem³

et al. 2011

Advances in Diverse Industrial Applications of Nanocomposites

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In vitro–in vivo characterization of gentamicin bone implants

Cited by 102 publications

References 28 publications

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Synthesis and characterisation of nanohydroxyapatite using an ultrasound assisted method

Multifunctional Nanocomposites Based on Mesoporous Silica: Potential Applications in Biomedicine

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