In vitro immunoglobulin response of fetal B-cells is influenced by perinatal infections and antibiotic treatment: a study in preterm infants

Cukrowská, Božena; Lodinová-Žádníková, R; Sokol, Deborah K.; Tlaskalová‐Hogenová, Helena

doi:10.1007/s004310051121

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…439 Premature neonates with perinatal infection or nosocomial infection may show signs of humoral immunoparalysis, manifested by decreased IgM/IgG production ex vivo as compared with production in their healthy age-matched counterparts. 440 Sepsis in early life did not reduce serum antibody titers in preterm infants after heptavalent pneumococcal conjugate vaccine exposure but was associated with a reduced opsonization titer to a single serotype, suggesting the capacity to respond to vaccination or other immune challenge may be altered. 441 In the setting of reduced classic adaptive immune function seen in early life as compared with the function in adults, innate lymphoid populations (which lack B cell receptor and T cell receptor) may play a significant role in protecting the neonate from infectious challenge.…”

Section: Innate Immune Cellular Contributionsmentioning

confidence: 88%

Pathophysiology of Neonatal Sepsis

Wynn¹,

Wong²

2017

Fetal and Neonatal Physiology

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Section: Innate Immune Cellular Contributionsmentioning

confidence: 88%

Pathophysiology of Neonatal Sepsis

Wynn¹,

Wong²

2017

Fetal and Neonatal Physiology

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“…Determination of the concentration of Ig. The concentration of total Ig of IgA and IgM isotypes was determined by the sandwich ELISA method as previously described [15] using isotype specific polyclonal Ab (Sevac). Newborn plasma was used in a twofold dilution starting with 1 : 25.…”

Section: Methodsmentioning

confidence: 99%

Specific Proliferative and Antibody Responses of Premature Infants to Intestinal Colonization with Nonpathogenic Probiotic E. coli Strain Nissle 1917

Cukrowská¹,

Lodinová-Žádníková²,

Enders³

et al. 2002

Scand J Immunol

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110

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The aim of this study was to analyze the influence of oral administration of E. coli Nissle 1917 on the systemic humoral and cellular immunity in premature infants. Thirty-four premature infants were colonized with E. coli Nissle 1917 in a randomized, placebo-controlled blinded clinical trial. Stool samples of infants were analyzed repeatedly for the presence of the administered strain. The proliferative response to bacterial antigens of E. coli origin was measured in whole blood of 34 colonized infants and 27 noncolonized controls. E. coli colonization induced a significant increase in the proliferation of blood cells cultivated with bacterial components of E. coli Nissle 1917 and another E. coli strain in colonized infants as compared with noncolonized controls. Significantly higher amounts of specific anti-E. coli Nissle 1917 antibodies (Ab) of immunoglobulin (Ig)A isotype and nonspecific polyclonal IgM were found in the blood of colonized infants compared to noncolonized placebo controls. We concluded that the oral application of E. coli Nissle 1917 after birth significantly stimulates specific humoral and cellular responses and simultaneously induces nonspecific natural immunity.

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“…As well as causing detrimental effects at the intestinal level, antibiotherapies may have an impact at the peripheral level. Recent study has shown that B-cell activity measured in ×itro is depressed in infants receiving antibiotic treatments (187).…”

Section: Discussionmentioning

confidence: 99%