In Vitro Identification of Gold Nanorods through Hyperspectral Imaging

Stacy, Bradley M.; Comfort, Kristen K.; Comfort, Donald A.; Hussain, Saber M.

doi:10.1007/s11468-013-9538-6

Cited by 18 publications

(9 citation statements)

References 26 publications

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“…Furthermore, semiquantitative analysis of cellular uptake of Au NPs was performed using dark-field microscopy. Dark-field microscopy is used to characterize the expression of scattered light from Au NPs that can be utilized to visualize Au NPs in living cells. , As shown in Figure c, it was obvious the cellular uptake level of Au NPs in DMEM was much higher than that in c-DMEM by both cell lines, and much more Au NPs were internalized by RAW 264.7 cells than Hep G2 cells. There was no significant difference in the cellular uptake of small-sized Au NPs for both cells lines.…”

Section: Resultsmentioning

confidence: 99%

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Cheng

Tian

et al. 2015

ACS Appl. Mater. Interfaces

255

212

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The interaction at nanobio is a critical issue in designing safe nanomaterials for biomedical applications. Recent studies have reported that it is nanoparticle-protein corona rather than bare nanoparticle that determines the nanoparticle-cell interactions, including endocytic pathway and biological responses. Here, we demonstrate the effects of protein corona on cellular uptake of different sized gold nanoparticles in different cell lines. The experimental results show that protein corona significantly decreases the internalization of Au NPs in a particle size- and cell type-dependent manner. Protein corona exhibits much more significant inhibition on the uptake of large-sized Au NPs by phagocytic cell than that of small-sized Au NPs by nonphagocytic cell. The endocytosis experiment indicates that different endocytic pathways might be responsible for the differential roles of protein corona in the interaction of different sized Au NPs with different cell lines. Our findings can provide useful information for rational design of nanomaterials in biomedical application.

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Section: Resultsmentioning

confidence: 99%

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Cheng

Tian

et al. 2015

ACS Appl. Mater. Interfaces

255

212

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“…For example, research is underway to develop AuNP-based procedures for drug and gene delivery, bioimaging techniques, sensor systems, cancer therapeutics, and vaccine development (Dykman and Khlebtsov 2012;Stacy et al 2013). AuNPs are viewed as advantageous over other materials for a multitude of reasons, including a general biocompatibility, standardized synthesis protocols, ease of functionalization, and distinctive plasmonic properties (Yeh et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Preliminary protein corona formation stabilizes gold nanoparticles and improves deposition efficiency

2015

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Due to their advantageous characteristics, gold nanoparticles (AuNPs) are being increasingly utilized in a vast array of biomedical applications. However, the efficacy of these procedures are highly dependent upon strong interactions between AuNPs and the surrounding environment. While the field of nanotechnology has grown exponentially, there is still much to be discovered with regards to the complex interactions between NPs and biological systems. One area of particular interest is the generation of a protein corona, which instantaneously forms when NPs encounter a protein-rich environment. Currently, the corona is viewed as an obstacle and has been identified as the cause for loss of application efficiency in physiological systems. To date, however, no study has explored if the protein corona could be designed and advantageously utilized to improve both NP behavior and application efficacy. Therefore, we sought to identify if the formation of a preliminary protein corona could modify both AuNP characteristics and association with the HaCaT cell model. In this study, a corona comprised solely of epidermal growth factor (EGF) was successfully formed around 10-nm AuNPs. These EGF-AuNPs demonstrated augmented particle stability, a modified corona composition, and increased deposition over stock AuNPs, while remaining biocompatible. Analysis of AuNP dosimetry was repeated under dynamic conditions, with lateral flow significantly disrupting deposition and the nano-cellular interface. Taken together, this study demonstrated the plausibility and potential of utilizing the protein corona as a means to influence NP behavior; however, fluid dynamics remains a major challenge to progressing NP dosimetry.

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“…As such, finding a mechanism to avoid or account for this spectral shift is a major challenge that must be overcome to progress the development of GNR-based biological applications. 23 To date, a few studies on nonendosomal delivery of gold nanospheres have been performed, 24 but none have evaluated delivery and integrity of more complex nanoparticles, such as GNRs. Several physical methods have been developed to actively delivery NMs to targeted cellular locations, thus avoiding endosomes and lysosomes, including sonoporation, Genegun, and microinjection techniques.…”

Section: ■ Introductionmentioning

confidence: 99%

Tannic Acid Coated Gold Nanorods Demonstrate a Distinctive Form of Endosomal Uptake and Unique Distribution within Cells

Untener

Comfort

Maurer

et al. 2013

ACS Appl. Mater. Interfaces

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One of the primary challenges associated with nanoparticle-dependent biological applications is that endosomal entrapment in a physiological environment severely limits the desired targeting and functionality of the nanoconstructs. This study sought to overcome that challenge through a systematic approach of gold nanorod (GNR) functionalization: evaluating the influence of both aspect ratio and surface chemistry on targeted cellular internalization rates and preservation of particle integrity. Owing to their unique spectral properties and enhanced surface area, GNRs possess great potential for the advancement of nanobased delivery and imaging applications. However, their ability for efficient intracellular delivery while maintaining their specific physiochemical parameters has yet to be satisfactorily explored. This study identified that longer and positively charged GNRs demonstrated a higher degree of internalization compared to their shorter and negative counterparts. Notably, of the four surface chemistries explored, only tannic acid resulted in retention of GNR integrity following endocytosis into keratinocyte cells, due to the presence of a strong protein corona matrix that served to protect the particles. Taken together, these results identify tannic acid functionalized GNRs as a potential candidate for future development in nanobased biomolecule delivery, bioimaging, and therapeutic applications.

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In Vitro Identification of Gold Nanorods through Hyperspectral Imaging

Cited by 18 publications

References 26 publications

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Preliminary protein corona formation stabilizes gold nanoparticles and improves deposition efficiency

Tannic Acid Coated Gold Nanorods Demonstrate a Distinctive Form of Endosomal Uptake and Unique Distribution within Cells

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