In vitro hydrolytic degradation of poly(ɛ-caprolactone) grafted dextran fibers and films

Bajgai, Madhab Prasad; Kim, Kawn-Woo; Parajuli, Daman Chandra; Yoo, Yeon Chun; Kim, Wan Doo; Khil, Myung-Seob; Kim, Hee Young

doi:10.1016/j.polymdegradstab.2008.08.002

Cited by 18 publications

(15 citation statements)

References 32 publications

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“…The degradation rate slowed down in the next month and about 18 wt % (17.86 ± 2.59) of the original weight lost after 2 months. Bajgai et al studied degradation of PCL grafted dextran nanofibers for 5 months (23 wt % loss) and showed that the weight loss was the highest during the first month and followed with slower degradation rate, compared to the results found here. Similarly, She et al demonstrated that degradation of silk fibroin/chitosan scaffolds slowed down after the first month.…”

Section: Resultssupporting

confidence: 49%

Incorporation of growth factor loaded microspheres into polymeric electrospun nanofibers for tissue engineering applications

Gungor-Ozkerim

Balkan

Köse

et al. 2013

J Biomedical Materials Res

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Nanofibrous double-layer matrices were prepared by electrospinning technique with the bottom layer formed from PCL (poly-ε-caprolactone)/PLLA (poly-l-lactic acid) nanofibers and the upper layer from PCL/Gelatin nanofibers. Bottom layer was designed to give mechanical strength to the system, whereas upper layer containing gelatin was optimized to improve the cell adhesion. Gelatin microspheres were incorporated in the middle of two layers for controlled growth factor delivery. Successful fabrication of the blend nanofibers were shown by spectroscopy. Scanning electron microscopy results demonstrated that bead-free nanofibers with uniform morphology could be obtained by 10% w/v concentrations of PCL/PLLA and PCL/Gelatin solutions. Microspheres prepared by 15% gelatin concentration and cross-linked with 7.5% glutaraldehyde solution were chosen after in vitro release studies for the incorporation to the double-layer matrices. The optimized conditions were used to prepare fibroblast growth factor-2 (FGF-2) loaded microspheres. Preliminary cell culture studies showed that the FGF-2 could be actively loaded into the microspheres and enhanced the cell attachment and proliferation. The complete system had a slow degradation rate in saline (18% weight loss in 2 months) and it could meanwhile preserve its integrity. This sandwich system prevented microsphere leakage from the scaffold, and the hydrophilic and bioactive nature of the fibers at the upper layer promoted cell attachment to the surface. PLLA/PCL layer, on the other hand, improved the mechanical properties of the system and enabled better handling.

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Section: Resultssupporting

confidence: 49%

Incorporation of growth factor loaded microspheres into polymeric electrospun nanofibers for tissue engineering applications

Gungor-Ozkerim

Balkan

Köse

et al. 2013

J Biomedical Materials Res

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“…XPS, yielding information on the outmost 50–100 Å of the surface, has been widely used to investigate polymer nanofibers prepared by electrospinning13–19 and to study the immobilization of proteins or other biomolecules onto the nanofibers 14–19. FTIR spectroscopy has also been widely employed in the characterization of polymer nanofibers,20–23 particularly of nanofibers containing immobilized biomolecules;17–19, 21–23 the possibility of investigating the peptide bond vibrations yields information on the immobilized protein structure 23–25…”

Section: Introductionmentioning

confidence: 99%

Biomimetic peptide‐enriched electrospun polymers: A photoelectron and infrared spectroscopy study

Iucci

Ghezzo

Danesin

et al. 2011

J of Applied Polymer Sci

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Biomimetic polymer nanofibers of poly(ecaprolactone) and poly(L-lactide-caprolactone) copolymer were prepared by electrospinning. Modifications of the polymer nanofibers aimed at improving their biomimetic properties were performed by two different routes: (1) immobilization of an adhesion peptide, which mimicked the adhesion sequence of the extracellular matrix protein fibronectin, on the polymer surface and (2) incorporation of self-complementary oligopeptides, which showed alternated hydrophilic and hydrophobic side chain groups and was capable of generating extended ordered structures by self-assembling, into the polymer nanofibers. The structure of the polymer/peptide nanofibers was investigated by X-ray photoelectron and Fourier transform infrared spectroscopies.

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“…The antimicrobial effects of the mats were significantly increased, up to 99%, by the addition of >30% chitosan (Table ). Since chitosan can adsorb electronegative substances in cells, it can disrupt the physiological activities of the bacteria, leading to bacterial cell death . In addition, the antimicrobial mechanism of chitosan may differ from that of other polysaccharides owing to the positive charges on the surface of chitosan .…”

Section: Resultsmentioning

confidence: 99%

“…Since chitosan can adsorb electronegative substances in cells, it can disrupt the physiological activities of the bacteria, leading to bacterial cell death. 22 In addition, the antimicrobial mechanism of chitosan may differ from that of other polysaccharides owing to the positive charges on the surface of chitosan. 23 In this study, we found that the antibacterial effects of different ratios of electrospun chitosan-PLGA-PEO on were similar between Gram-positive and Gram-negative bacteria.…”

Section: Antimicrobial Testsmentioning

confidence: 99%

Nanofiber mats composed of a chitosan-poly(d ,l -lactic-co -glycolic acid)-poly(ethylene oxide) blend as a postoperative anti-adhesion agent

Kim

et al. 2016

J. Biomed. Mater. Res.

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Postoperative tissue adhesion causes serious complications and suffering in 90% of patients after peritoneum surgery, while commercial anti-adhesion agents cannot completely prevent postoperative peritoneal adhesions. This study demonstrates electrospining of a blended solution of chitosan, poly(d,l-lactic-co-glycolic acid) (PLGA), and poly(ethylene oxide) (PEO) to fabricate a chitosan-based nanofibrous mat as a postoperative anti-adhesion agent. Rheological studies combined with scanning electron microscopy reveal that the spinnability of the chitosan-PLGA solution could be controlled by adjusting the blend ratio and concentration with average fiber diameter from 634 to 913 nm. Biodegradation of the nanofiber specimens showed accelerated hydrolysis by chitosan. Proliferation of fibroblasts and antimicrobial activity of nanofibers containing chitosan was analyzed. Abdominal defects with cecum adhesion in rats demonstrated that the blend nanofiber mats were effective in preventing tissue adhesion as a barrier (4 weeks after abdominal surgery) by coverage of exfoliated peritoneum and insufficient wound sites at the beginning of the wound healing process. Chitosan-PLGA-PEO blend nanofiber mats will provide a promising key as a postoperative anti-adhesion agent. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1906-1915, 2017.

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In vitro hydrolytic degradation of poly(ɛ-caprolactone) grafted dextran fibers and films

Cited by 18 publications

References 32 publications

Incorporation of growth factor loaded microspheres into polymeric electrospun nanofibers for tissue engineering applications

Incorporation of growth factor loaded microspheres into polymeric electrospun nanofibers for tissue engineering applications

Biomimetic peptide‐enriched electrospun polymers: A photoelectron and infrared spectroscopy study

Nanofiber mats composed of a chitosan-poly(d ,l -lactic-co -glycolic acid)-poly(ethylene oxide) blend as a postoperative anti-adhesion agent

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