In Vitro HIV Type-1 Reverse Transcriptase Inhibitory Activity from Leaf Extracts of<i>Scoparia dulcis</i>L.

Porika, Mahendar; Aileni, Mahender; Kokkirala, Venugopal Rao; Gadidasu, Kranthi Kumar; Umate, Pavan; Allini, Venkateswar Rao; Devarakonda, Rama Krishna; Abbagani, Sadanandam

doi:10.1080/10496470903378854

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…This plant has been intensively studied as a traditional herbal medicine (Latha et al 2004). Previous studies have revealed that the presence of numerous phytochemicals, such as coumarins, saponins, tannins, flavonoids and various terpenoids like scoparic acid A, scoparic acid B, scopadulciol and scopadulin, in this plant species are responsible for its medicinal properties (Latha et al 2006(Latha et al , 2009Babincová et al 2008;Riel et al 2002;Porika et al 2009;Ahsan et al 2003). Some of these properties include antitumor-promoting activity, antihyperalgesic, antimalarial, antiulcer, antipyretic and aphrodisiac activities, as well as cytotoxicity activity against cancer cells (Taylor 2006).…”

Section: Introductionmentioning

confidence: 98%

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Mahender

Abbagani

Zhang

2011

Plant Biotechnol Rep

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Efficient Agrobacterium-mediated genetic transformation of Scoparia dulcis L. was developed using Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pCAMBIA1301 with b-glucuronidase (GUS) (uidA) and hygromycin phosphotransferase (hpt) genes. Two-day precultured leaf segments of in vitro shoot culture were found to be suitable for cocultivation with the Agrobacterium strain, and acetosyringone was able to promote the transformation process. After selection on shoot organogenesis medium with appropriate concentrations of hygromycin and carbenicillin, adventitious shoots were developed on elongation medium by twice subculturing under the same selection scheme. The elongated hygromycin-resistant shoots were subsequently rooted on the MS medium supplemented with 1 mg l -1 indole-3-butyric acid and 15 mg l -1 hygromycin. Successful transformation was confirmed by PCR analysis using uidA-and hpt-specific primers and monitored by histochemical assay for b-GUS activity during shoot organogenesis. Integration of hpt gene into the genome of transgenic plants was also verified by Southern blot analysis. High transformation efficiency at a rate of 54.6% with an average of 3.9 ± 0.39 transgenic plantlets per explant was achieved in the present transformation system. It took only 2-3 months from seed germination to positive transformants transplanted to soil. Therefore, an efficient and fast genetic transformation system was developed for S. dulcis using an Agrobacteriummediated approach and plant regeneration via shoot organogenesis, which provides a useful platform for future genetic engineering studies in this medicinally important plant.

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Section: Introductionmentioning

confidence: 98%

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Mahender

Abbagani

Zhang

2011

Plant Biotechnol Rep

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“…Hispidulin, a steroidal glycoside isolated using an aqueous extract of S. dulcis exhibited anti HIV activity (Osei-Safo et al 2009). HIV type 1 reverse transcriptase inhibitory activity of methanolic extracts of leaf was also reported (Porika et al 2009).…”

Section: Introductionmentioning

confidence: 89%

Stable plastid transformation in Scoparia dulcis L.

Narra

Kota

Kumar

et al. 2016

Physiol Mol Biol Plants

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In the present investigation we report stable plastid transformation in L., a versatile medicinal herb via particle gun method. The vector KNTc, harbouring as a selectable marker and as a reporter gene which were under the control of synthetic promoter pNG1014a, targets inverted repeats,/ of the plastid genome. By use of this heterologous vector, recovery of transplastomic lines with suitable selection protocol have been successfully established with overall efficiency of two transgenic lines for 25 bombarded leaf explants. PCR and Southern blot analysis demonstrated stable integration of foreign gene into the target sequences. The results represent a significant advancement of the plastid transformation technology in medicinal plants, which relevantly implements a change over in enhancing and regulating of certain metabolic pathways.

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“…Hydroxamic acid has been isolated from dried roots and aerial parts (Chiu-Ming and Ming-Tyan 1976), which exhibits insecticidal, antifungal and antibacterial activity. Moreover, our research group evaluated its efficacy towards reverse transcriptase (Porika et al 2009). In view of its wide ethnomedical acceptance it has attracted the attention of scientific community for its therapeutical values and thus remained as subject of intensified research.…”

Section: Introductionmentioning

confidence: 99%

Biolistic transformation of Scoparia dulcis L.

Kota

Narra

Kumar

et al. 2016

Physiol Mol Biol Plants

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Here, we report for the first time, the optimized conditions for microprojectile bombardment-mediated genetic transformation in Vassourinha (Scoparia dulcis L.), a Plantaginaceae medicinal plant species. Transformation was achieved by bombardment of axenic leaf segments with Binary vector pBI121 harbouring β-glucuronidase gene (GUS) as a reporter and neomycin phosphotransferase II gene (npt II) as a selectable marker. The influence of physical parameters viz., acceleration pressure, flight distance, gap width & macroprojectile travel distance of particle gun on frequency of transient GUS and stable (survival of putative transformants) expressions have been investigated. Biolistic delivery of the pBI121 yielded the best (80.0 %) transient expression of GUS gene bombarded at a flight distance of 6 cm and rupture disc pressure/acceleration pressure of 650 psi. Highest stable expression of 52.0 % was noticed in putative transformants on RMBI-K medium. Integration of GUS and npt II genes in the nuclear genome was confirmed through primer specific PCR. DNA blot analysis showed more than one transgene copy in the transformed plantlet genomes. The present study may be used for metabolic engineering and production of biopharmaceuticals by transplastomic technology in this valuable medicinal plant.

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In Vitro HIV Type-1 Reverse Transcriptase Inhibitory Activity from Leaf Extracts ofScoparia dulcisL.

Cited by 5 publications

References 17 publications

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Stable plastid transformation in Scoparia dulcis L.

Biolistic transformation of Scoparia dulcis L.

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