In Vitro Hatching of Ascaris suum Eggs

Jaskoski, Benedict J.; Colucci, Anthony V.

doi:10.2307/3224740

Cited by 32 publications

(14 citation statements)

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“…A similar result was reported by Fairbairn (1961), who demonstrated in vitro hatching of A. lumbricoides eggs after the addition of sulfur dioxide, sodium chloride, and sodium bicarbonate to an atmosphere of 5% carbon dioxide and 95% nitrogen. Jaskoski and Colucci (1964) tested the eect of ox bile on egg hatching and found that hatching in the presence of 90% bile failed to produce viable larvae, regardless of carbon dioxide supplementation, whereas a 10% bile concentration resulted in low hatching percentages (25±50%).…”

Section: Discussionmentioning

confidence: 99%

“…The incubation hatching method was modi®ed from the method reported by Jaskoski and Colucci (1964). Infective eggs were washed twice in Hedon-Fleig's solution and then diluted to a concentration of 5 eggs/ll, and 400 ll was transferred to each of 24 wells on a plate.…”

Section: Methods 2: Incubation Hatching Methodsmentioning

confidence: 99%

“…Hatching of A. lumbricoides eggs in vitro has been achieved in an atmosphere of carbon dioxide and reducing agents (Rogers 1958) or using sulfur dioxide, sodium chloride, and sodium bicarbonate in an atmosphere of 5% carbon dioxide and 95% nitrogen (Fairbairn 1961). In other studies, A. suum eggs have been hatched using high carbon dioxide concentrations without reducing agents (Jaskoski and Colucci 1964), carbon dioxide combined with glass beads and magnetic stirring (Stromberg and Soulsby 1976), or glass beads with magnetic stirring in the absence of carbon dioxide (Urban et al 1981).…”

Section: Introductionmentioning

confidence: 99%

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Effects of bile on the in vitro hatching, exsheathment, and migration of Ascaris suum larvae

Han¹,

Eriksen²,

Boes³

et al. 2000

Parasitol Res

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This study reports on the in vitro egg hatching, exsheathment, migratory activity, and sensitivity to anthelmintics of Ascaris suum larvae in cultures with or without bovine bile. Three methods for egg hatching and/or incubation were used: an object-glass method, a glass-bead method, and an incubation method. An agar migration assay (AMA) was developed to test the migratory activity of Ascaris larvae following hatching. Bile appeared to be an important stimulatory factor for both egg hatching and larval mobility in the incubation method. Incubation in low concentrations of bile (2%, 5%, or 10%) stimulated both egg hatching and larval migration, whereas concentrations of at least 20% impaired egg hatching and larval migration. Furthermore, 5% bile seemed to promote exsheathment of A. suum larvae.

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Section: Discussionmentioning

confidence: 99%

Section: Methods 2: Incubation Hatching Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of bile on the in vitro hatching, exsheathment, and migration of Ascaris suum larvae

Han¹,

Eriksen²,

Boes³

et al. 2000

Parasitol Res

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“…Normal guinea pigs under Halothane anesthesia were given intravitreal injections of 0.1 ml of suspensions containing 3,000 artificially hatched infective second stage larvae (L2) of A. suum [Jaskoski and Colucci, 1964], 50 fourth stage larvae (L4) of A. suum produced in vitro / Stromberg et al, 1977] or 500 artificially hatched L2 of T. canis, in sterile phosphatebuffered saline (pH 7.0), or 10 pi (3.1 pg protein) of an antigen of A. suum (ACF antigen) produced in vitro in defined medium during the metamorphosis of L3 to L4 [Stromberg and Soulsby, 1977], Anterior chamber paracentesis was performed at weekly intervals after infection, as previously described, and the fluid along with serum taken at the same time were tested by the 6-day passive cutaneous anaphylaxis (P-K) technique for specific IgE antibodies [Dobson et al, 1971;Donnelly et al, 1977] using antigens prepared from the adult stages of A. suum or T. canis, or the ACF antigen obtained from in vitro culture of larvae of A. suum as described above.…”

Section: Methodsmentioning

confidence: 99%

Intraocular Immunoglobulin E Induced by Intravitreal Infection with Ascaris and Toxocara spp. Larvae

Soulsby¹,

Stromberg²,

Donnelly³

et al. 1980

Ophthalmic Res

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Intravitreal injection of Toxocara canis and Ascaris suum larvae into guinea pigs is being studied as a model for human ocular toxocariasis. IgE antibody-mediated mechanisms may be of importance in the ocular immunopathologic reaction to ascarid parasites. Intravitreal injection of second and fourth stage A. suum larvae, second stage T. canis larvae, or a soluble antigen obtained from cultures in defined media of third stage A. suum molting to the fourth stage (ACF antigen) was followed by the appearance of IgE antibody in aqueous humor and serum, detectable by the 6-day passive cutaneous anaphylactic (P-K) test. Animals systemically immunized to the ACF antigen and possessing serum IgE antibody titers of 1:1,000 to 1:10,000 had little or no IgE antibody in their aqueous humor. The ability of soluble antigens to provoke systemic and intraocular IgE antibody responses suggests that antigens released by living larvae may be important in this process. The observation that intraocular IgE antibodies occur in some animals lacking detectable serum IgE antibody indicates that the intraocular IgE antibody is locally produced.

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“…Em bryonated eggs were deshelled using the m ethod of Haskins and W einstein (16), hatched by the method of Jaskoski and Colucci (20), and cultured in sterile bottles in an atm osphere of C 02 a t 37°C for 12 and 16 days. Eagle's minimum essential medium (MEM) plus 10% antibiotic m ixture (both from G rand Island Biological Co; 3175 Staley Road, G rand Island, N.Y .…”

Section: Preparation Of Antigensmentioning

confidence: 99%

Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae

Guerrero

Silverman

1973

Rev. Soc. Bras. Med. Trop.

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INTRODU CTIONAscaris suum larval metabolic fluids nave been shown to be good protective antigens when injected into mice, prior to an infection (13,14,15). Because of the complexity of the medium in whicíi the larvae ihave to be grown (23) there m ight bs a dim inution of the immune response due to the presence of competing nonprotective immunogens.It is well known th a t the sim ultaneous exposure of an anim al to several antigens decreases the immune response to some of the antigens in the m ixture (1,2,11, 12). It is, therefore, desirable to separate a complex antigenic m ixture into its components. One of the preferred methods with the least biological damage to the antigens is fractionation on the basis of molecular size differences using filtration through a column of agarose gels. These are more rigid th a n m any other gels and have low compressibility so th a t a good flow rate and reproducible separation migih t bs insured (18).The present paper concerns attem pts to isolate the A. suum metabolic larval intigens from the culture medium using exclusion chrom atography. MATERIALS AND METHODSAnimais. One hundred and ten albino Swiss mice averaging 22.2 ± 1.5 gm in weight were divided into 11 groups of 10 anim ais ea ch .T h is stu d y w as p e rfo rm e d a t th e D e p a rtm e n t of Zoology, U n iv e rsity of Illin o is, U rb a n a , Illin o is, U .S .A ., u n d e r p a r tia l su p p o rt of g r a n t AI05910 from th e N a tio n a l I n s t it u t o of Allergy a n d In fe c tio u s Diseases. * C e n tro d e P e sq u isa s em F isio lo g ia e Im u n o lo g ia de H elm in to s, D e p a rta m e n to de M icrobiologia e Parasito lo g ia, E scola P a u lis ta de M ed icin a. São P a u lo . B rasil. ** D e p a rtm e n t o f Biology, U n iv ersity of New M exico, A lb u q u erq u e, New M exico. U .S .A . S u b m itte d to p u b lic a tio n on 6.25.73.

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In Vitro Hatching of Ascaris suum Eggs

Cited by 32 publications

References 0 publications

Effects of bile on the in vitro hatching, exsheathment, and migration of Ascaris suum larvae

Effects of bile on the in vitro hatching, exsheathment, and migration of Ascaris suum larvae

Intraocular Immunoglobulin E Induced by Intravitreal Infection with Ascaris and Toxocara spp. Larvae

Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae

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