In vitro growth stimulatory and in vivo wound healing studies on cycloartane-type saponins of Astragalus genus

Sevimli-Gur, Canan; Onbaşılar, İlyas; Atilla, Pergin; Genç, Rükan; Çakar, Nur; Deliloglu-Gurhan, Ismet; Bedir, Erdal

doi:10.1016/j.jep.2011.01.030

Cited by 63 publications

(37 citation statements)

References 18 publications

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“…Although EGF exhibits a promising wound closure effect, its short half-life in the wound bed limits its widespread clinical usage. In the present study, treating scratched HEKn cell monolayers with CAG accelerated scratch wound closure, which is consistent with a recent report on the wound closure effect of this molecule [51]. Although CAG did not affect HEKn cell migration, it induced the proliferation of HEKn cells, which can contribute to scratch wound closure.…”

Section: Discussionsupporting

confidence: 93%

Cycloastragenol Is a Potent Telomerase Activator in Neuronal Cells: Implications for Depression Management

Ip¹,

Ng²,

An³

et al. 2014

Neurosignals

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Cycloastragenol (CAG) is an aglycone of astragaloside IV. It was first identified when screening Astragalus membranaceus extracts for active ingredients with antiaging properties. The present study demonstrates that CAG stimulates telomerase activity and cell proliferation in human neonatal keratinocytes. In particular, CAG promotes scratch wound closure of human neonatal keratinocyte monolayers in vitro. The distinct telomerase-activating property of CAG prompted evaluation of its potential application in the treatment of neurological disorders. Accordingly, CAG induced telomerase activity and cAMP response element binding (CREB) activation in PC12 cells and primary neurons. Blockade of CREB expression in neuronal cells by RNA interference reduced basal telomerase activity, and CAG was no longer efficacious in increasing telomerase activity. CAG treatment not only induced the expression of bcl2, a CREB-regulated gene, but also the expression of telomerase reverse transcriptase in primary cortical neurons. Interestingly, oral administration of CAG for 7 days attenuated depression-like behavior in experimental mice. In conclusion, CAG stimulates telomerase activity in human neonatal keratinocytes and rat neuronal cells, and induces CREB activation followed by tert and bcl2 expression. Furthermore, CAG may have a novel therapeutic role in depression. © 2014 S. Karger AG, Basel

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Section: Discussionsupporting

confidence: 93%

Cycloastragenol Is a Potent Telomerase Activator in Neuronal Cells: Implications for Depression Management

Ip¹,

Ng²,

An³

et al. 2014

Neurosignals

View full text Add to dashboard Cite

show abstract

“…The scratch assay has been used to study proliferation/migration properties of different cell lines treated with extracts from species of e.g., the genera Astragalus, Calendula, Hemigraphis, Matricaria and Simmondsia Edwin and Nair, 2011;Ranzato et al, 2011;Sevimli-Gur et al, 2011). A stimulation of cell proliferation and migration was reported for extracts from all of these species.…”

Section: Wound Healing Properties Evaluated With the Scratch Assaymentioning

confidence: 99%

Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay

Zubaır

Ekholm

Nybom

et al. 2012

Journal of Ethnopharmacology

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“…1) contained in AR may have the therapeutic potential for wound treatment. Interestingly, Sevimli-Gür et al (2011) recently reported the increasing effect of astragalosides IV on keratinocytes viability and the promotion of the epithelization and inflammation in the granulation tissue in rat skin lesions. However, the underlying mechanism remains largely unknown and deserves further investigations.…”

Section: Introductionmentioning

confidence: 99%

The healing and anti-scar effects of astragaloside IV on the wound repair in vitro and in vivo

Chen

Peng

et al. 2012

Journal of Ethnopharmacology

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In vitro growth stimulatory and in vivo wound healing studies on cycloartane-type saponins of Astragalus genus

Cited by 63 publications

References 18 publications

Cycloastragenol Is a Potent Telomerase Activator in Neuronal Cells: Implications for Depression Management

Cycloastragenol Is a Potent Telomerase Activator in Neuronal Cells: Implications for Depression Management

Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay

The healing and anti-scar effects of astragaloside IV on the wound repair in vitro and in vivo

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