In vitro Growth of Oesophagostomum dentatum (Nematoda: Strongyloidea) from Third-Stage Larvae to Adults with Observations on Inhibited Larval Development

Douvres, F. W.

doi:10.2307/3276554

Cited by 4 publications

(4 citation statements)

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“…According to previous reports, fewer L4 develop when blood serum of other animal species is used instead of pig serum (Schulz and Dalchow 1969;Leland 1970). Douvres (1966) obtained development rates of only 1±3% in medium containing sheep serum. Leland (1970) reported development rates for O. quadrispinulatum L3 to L4 of more than 80% in medium with pig serum.…”

Section: Resultsmentioning

confidence: 92%

“…L4 hatched in BM-C (trial 10) had a mean length of 2,014 lm and a mean width of 103 lm; the respective values for L4 of group B (BM) were 2,055 lm and 103 lm (n 30 in each group). Generally, embryo extracts, in most cases of chicken origin, are used as a main medium ingredient (30±50% of complete medium) for in vitro cultivation of nodular worms of pigs (Douvres 1966;Eckert 1967;Schulz and Dalchow 1969;Leland 1970;Daugschies 1995). According to Tarakamov and Kaarma (1973), O. dentatum develops to the fourth and ®fth stage in a complex medium with only 5% CEHE.…”

Section: Resultsmentioning

confidence: 99%

“…After 28 days the L4 of group B (BM-C) were longer (average 2,581 lm, maximum 3,892 lm, n 50) than those of group A (average 2,423 lm, maximum 3,047 lm, n 50; P < 0.05). Liver extract is commonly used as a medium ingredient (Douvres 1966;Tarakanov and Kaarma 1973;Daugschies 1995Daugschies , 1996 and is important for in vitro cultivation. The animal species from which the extract originates is not of considerable impact (Schulz and Dalchow 1969).…”

Section: Resultsmentioning

confidence: 99%

“…The histotropic fourth-stage larvae (L4) are valuable tools for in vitro application, e.g., for screening of drugs or for physiology and immunology studies (Daugschies 1995(Daugschies , 1996. In vitro cultivation procedures have been described for O. dentatum (Douvres 1966;Tarakanov andKaarma 1972, 1973;Tarakanov 1974;Daugschies 1995). The aim of the present study was to reconsider established cultivation procedures critically and to de®ne essential conditions for in vitro development of O. dentatum to the parasitic L4 stage.…”

Section: Introductionmentioning

confidence: 99%

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In vitro development of histotropic larvae of Oesophagostomum dentatum under various conditions of cultivation

Daugschies¹,

Watzel²

1999

Parasitology Research

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We performed a total of 14 trials to evaluate the culture conditions suited best for the in vitro production of fourth-stage larvae (L4) of Oesophagostomum dentatum. Chicken embryo extract was shown to be redundant, whereas pig blood serum, trypticase, liver extract, and yeast extract proved to be important medium ingredients. Development to L4 could be moderately accelerated by increases of temperature (40 degrees C) and of CO2 concentration (20% in air), but the total yield of L4 could not be improved. Thus, conditions of 38.5 degrees C and 10% CO2 are recommended for routine application.

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Section: Resultsmentioning

confidence: 92%