Oesophagostomum quadrispinulatum: In Vitro Development of Parasitic Stages

In vitro development of histotropic larvae of Oesophagostomum dentatum under various conditions of cultivation

“…The maximal L4 yield was reached in group B at 12.8% at the end of the cultivation period of 28 days and in group A at only 1.2% on day 19. According to previous reports, fewer L4 develop when blood serum of other animal species is used instead of pig serum (Schulz and Dalchow 1969;Leland 1970). Douvres (1966) obtained development rates of only 1±3% in medium containing sheep serum.…”

“…Douvres (1966) obtained development rates of only 1±3% in medium containing sheep serum. Leland (1970) reported development rates for O. quadrispinulatum L3 to L4 of more than 80% in medium with pig serum. We found in preliminary studies that O. quadrispinulatum develops like O. dentatum in BM-C to the L4 stage, whereas this culture system is not suited for O. radiatum of cattle and O. venulosum of sheep (data not shown).…”

“…L4 hatched in BM-C (trial 10) had a mean length of 2,014 lm and a mean width of 103 lm; the respective values for L4 of group B (BM) were 2,055 lm and 103 lm (n 30 in each group). Generally, embryo extracts, in most cases of chicken origin, are used as a main medium ingredient (30±50% of complete medium) for in vitro cultivation of nodular worms of pigs (Douvres 1966;Eckert 1967;Schulz and Dalchow 1969;Leland 1970;Daugschies 1995). According to Tarakamov and Kaarma (1973), O. dentatum develops to the fourth and ®fth stage in a complex medium with only 5% CEHE.…”