In vitro growth characteristics of Fusarium langsethiae isolates recovered from oats and wheat grain in the UK

Imathiu, Samuel; Ray, Rumiana V.; Back, Matthew A.; Hare, M. C.; Edwards, Simon

doi:10.1556/038.51.2016.2.1

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…On artificial media, the optimum temperature for mycelial growth of FP was reported to be 20-25°C (Brennan et al, 2003), whereas for FL, it was shown to be best at 25°C (Medina & Magan, 2010). Similar results were obtained by Imathiu et al (2016); however, they also reported that FL growth was possible at 10°C.…”

Section: Discussionmentioning

confidence: 76%

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Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

et al. 2018

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High occurrence of Fusarium poae (FP) and Fusarium langsethiae (FL) and their mycotoxins nivalenol (NIV) and T‐2/HT‐2 have been observed in Swiss oats. Early prediction of mycotoxin levels is important for farmers and the cereal industry to minimize the risk of contaminated food and feed. Therefore, climate chamber experiments were conducted to investigate the influence of different temperatures (10, 15, 20 °C) and durations (4, 8, 12 h) at 99% relative humidity (RH) on the infection of oats with FP and FL. In addition, to discover the most susceptible period of oats, artificial FL inoculations were conducted at different growth stages. Field experiments were performed to observe the dispersal of these fungal species within the field and to investigate the weather conditions that influence the dispersal. The climate chamber experiments revealed higher contamination with NIV and T‐2/HT‐2 in the 10 °C treatments and with a prolonged humidity duration of 12 h 99% RH. Inoculations of oat plants at early (DC 61) and mid (DC 65) anthesis, led to higher FL infection and T‐2/HT‐2 accumulation in the grains compared with treatments at earlier growth stages, which might be due to an increased susceptibility during anthesis. No indication for spore dispersal was observed in the field experiments. The results obtained, together with the cropping factors that influence infection and mycotoxin production, could be used as a first step in developing forecasting models to predict the contamination of oats with the mycotoxins NIV and T‐2/HT‐2.

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Section: Discussionmentioning

confidence: 76%

“…Similar results were obtained by Imathiu et al . (); however, they also reported that FL growth was possible at 10 °C.…”

Section: Discussionmentioning

confidence: 97%

Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

et al. 2018

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“…This finding is in line with a study by Bernhoft et al 2012 who reported a positive relationship between F. langsethiae and the mean July temperature (typically corresponding to the flowering time of oats in Norway). Fusarium langsethiae has an optimum growth rate between 20°C and 24°C (Imathiu et al 2016, Nazari et al 2014). This suggests that increased temperatures, within the range of temperatures normally observed in Norway and UK, will stimulate the growth of F. langsethiae, thereby increasing the risk of elevated HT2 + T2 levels in oats.…”

Section: Discussionmentioning

confidence: 99%

HT-2 and T-2 toxins in Norwegian oat grains related to weather conditions at different growth stages

et al. 2018

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High concentrations of the mycotoxins HT-2 and T-2 (HT2 + T2), primarily produced by Fusarium langsethiae, have occasionally been detected in Norwegian oat grains. In this study, we identified weather variables influencing accumulation of HT2 + T2 in Norwegian oat grains. Oat grain samples from farmers' fields were collected together with weather data (2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012)(2013). Spearman rank correlation coefficients were calculated between the HT2 + T2 contamination in oats at harvest and a range of weather summarisations within estimated phenological windows of growth stages in oats (tillering, flowering etc.). Furthermore, we developed a mathematical model to predict the risk of HT2 + T2 in oat grains. Our data show that adequate predictions of the risk of HT2 + T2 in oat grains at harvest can be achieved, based upon weather data observed during the growing season. Humid and cool conditions, in addition to moderate temperatures during booting, were associated with increased HT2 + T2 accumulation in harvested oat grains, whereas warm and humid weather during stem elongation and inflorescence emergence, or cool weather and absence of rain during booting reduced the risk of HT2 + T2 accumulation. Warm and humid weather immediately after flowering increased the risk, while moderate to warm temperatures and absence of rain during dough development, reduced the risk of HT2 + T2 accumulation in oat grains. Our data indicated that HT2 + T2 contamination in oats is influenced by weather conditions both pre-and post-flowering. These findings are in contrast with a previous study examining the risk of deoxynivalenol contamination in oat reporting that toxin accumulation was mostly influenced by weather conditions from flowering onwards.

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“…Toxin production by FL strains occurred over 10-35°C with an optimal range of 20-30°C (Nazari et al 2014). For isolates from oat in north-western Europe, the optimal range was found to be the same (Medina and Magan 2011;Kokkonen et al 2012b;Imathiu et al 2016). The growth and toxin production by FL were reduced by water activity (a w ) when it fell below 0.98, and at 0.90-0.93 the growth rate was very low (Medina and Magan 2011;and Kokkonen et al 2012b).…”

Section: Introductionmentioning

confidence: 91%

Contrasting responses of T-2, HT-2 and DON mycotoxins and Fusarium species in oat to climate, weather, tillage and cereal intensity

et al. 2019

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Analysis of survey data from 804 spring-oat fields divided into five climatic regions suggested that low inoculum level of Fusarium langsethiae was a major limiter of T-2 + HT-2. A 30-year climate with a cool and rainy 10-day period preceding estimated mid-anthesis and a warm 3-week period following anthesis were positively associated with T-2 + HT-2 contamination. In 12 survey years, warm weather from 4 weeks before GS65 until harvesting increased T-2 + HT-2, except in the 1-2 weeks preceding mid-anthesis, when the requirement for high humidity dominated. F. langsethiae and F. sporotrichioides were consistently promoted by high temperature from mid-anthesis onwards. A positive response of T-2 + HT-2 to high humidity peaked 5-10 days earlier and was shorter and weaker than the responses of DON and F. graminearum. The probability of high concentration and regional mean concentration of T-2 + HT-2 tended to be lower under ploughing than under nonploughing, and they tended to increase with increasing cereal intensity. T-2 + HT-2 was positively associated with zero tillage when compared with minimum tillage. The responses of F. langsethiae and F. sporotrichioides to ploughing and cereal intensity varied by region. In contrast to T-2 + HT-2, DON was the same or higher under ploughing than under non-ploughing, not consistently affected by cereal intensity and not associated with zero tillage. F. graminearum was consistently more common under ploughing than under non-ploughing, and decreased with increasing cereal intensity in four regions. Also, F. culmorum and F. poae tended to be more common under ploughing. F. culmorum increased with cereal intensity in three regions.

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In vitro growth characteristics of Fusarium langsethiae isolates recovered from oats and wheat grain in the UK

Cited by 8 publications

References 21 publications

Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

HT-2 and T-2 toxins in Norwegian oat grains related to weather conditions at different growth stages

Contrasting responses of T-2, HT-2 and DON mycotoxins and Fusarium species in oat to climate, weather, tillage and cereal intensity

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