In-vitro grafting – Current applications and future prospects

Chilukamarri, Laxmi; Ashrafzadeh, Seyedardalan; Leung, David W. M.

doi:10.1016/j.scienta.2021.109899

Cited by 22 publications

(17 citation statements)

References 50 publications

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“…Until now, little information has been available regarding the RNAs and proteins responsible for biosynthesis due to the lack of attention paid to secondary metabolites. It has been demonstrated that extensive movability of RNAs and proteins occurs in model plants like Arabidopsis and tobacco [ 10 – 14 ], indicating the possibility of transfer of these RNAs and proteins, which are closely connected with secondary metabolite biosynthesis. Therefore, the accumulation of particular target compounds could be potentially reflected by the transport of the metabolites, RNA, proteins, and other factors associated with their biosynthesis.…”

Section: The Accumulation Of Secondary Metabolites Is Regulated By Mu...mentioning

confidence: 99%

Grafting: a potential method to reveal the differential accumulation mechanism of secondary metabolites

Dong

Shi

Mou

et al. 2022

Horticulture Research

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Plant secondary metabolites have a great contribution to the agricultural and pharmaceutical industries. Their accumulation is determined by the integrated transport of target compounds and their biosynthesis-related RNA, protein, or DNA. However, it is hard to track the movement of these biomolecules in vivo. Grafting may be an ideal method to solve this problem. The differences of genetic and metabolic backgrounds in rootstock and scion make it feasible to determine the movement of target compounds, RNAs, proteins, and DNA coupled with multiple omics approaches and other molecular tools. In this review, we will introduce how to use the grafting technique, together with molecular biological tools, to reveal the differential accumulation mechanism of plant secondary metabolites from different levels. A detailed case on the transport of one diterpene alkaloid, fuziline, will be further illustrated to clarify how to reveal the specific accumulation model is shaped with the help of grafting and multiple molecular biological tools.

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Section: The Accumulation Of Secondary Metabolites Is Regulated By Mu...mentioning

confidence: 99%

Grafting: a potential method to reveal the differential accumulation mechanism of secondary metabolites

Dong

Shi

Mou

et al. 2022

Horticulture Research

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“…Over the past decades, various methods have been developed for pathogen eradication from the infected plants, such as shoot tip (meristem) culture, micrografting and a combination of thermotherapy, cold therapy or chemotherapy with shoot tip culture (Barba et al, 2017; Chilukamarri et al, 2021; Laimer & Barba, 2011; Panattoni et al, 2013; Wang, Cui, et al, 2018). A combination of thermotherapy with shoot tip culture significantly improved virus eradication (Wang, Cui, et al, 2018).…”

Section: Needs For Pathogen‐free Plantsmentioning

confidence: 99%

Shoot tip cryotherapy for plant pathogen eradication

Bettoni

Zhang

et al. 2022

Plant Pathology

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Diseases caused by plant pathogens such as viruses, viroids and phytoplasmas cause huge economic losses of agricultural production and limit the safe movement of plant materials across borders. The use of pathogen-free planting materials provides a strategy for efficient management of these diseases and facilitates the global exchange of genetic resources. Shoot tip cryotherapy is a novel biotechnology method that uses cryogenic procedures to eradicate plant pathogens from the diseased plants.Combining thermotherapy or chemotherapy with shoot tip cryotherapy has further enhanced pathogen eradication efficiency. This review provides updated and comprehensive information on shoot tip cryotherapy and the combination of thermotherapy or chemotherapy with shoot tip cryotherapy for pathogen eradication. Prospects are proposed for future studies.

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“…Following the advent of in vitro plant tissue culturing in the early 1900s [7], a grafting system using tissue culture (micrografting) was first demonstrated by Doorenbos [8] in ivy and then Holmes [9] in chrysanthemum in the 1950s, and was later developed and standardized for virus eradication from citrus species by Murashige et al [10] and Navarro et al [11]. To date, in vitro micrografting (IVM) has been widely applied (1) in pathogen management to facilitate the eradication, indexing and transmission of pathogens, as well as the assessments of graft incompatibility induced by pathogen infection [11][12][13][14][15]; (2) to facilitate in vitro rooting [16][17][18][19], to invigorate regenerating plant tissue cultures during micropropagation [19][20][21][22], and for the rapid assessment of graft compatibility [23][24][25][26]; and (3) in studies focusing on the molecular mechanism of graft compatibility, as well as the exchange and trafficking of macromolecules between scions and rootstocks [27][28][29][30].…”

Section: Overall Developments and Characters Of Micrograftingmentioning

confidence: 99%

“…For example, IVM of microshoots onto rootstock seedlings was applied to solve the in vitro recalcitrance of Protea cynaroides, an important ornamental species endemic to South Africa [55]; Lens culinaris, an important pulse crop of Mediterranean area [16]; and some Prunus species [21,33,55,66]. The development of suitable micrografting techniques to overcome rooting difficulties of plant species has been highlighted in previous reviews [15,105,106].…”

Section: Root Promotionmentioning

confidence: 99%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

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In vitro micrografting is an important technique supporting the micropropagation of a range of plant species, particularly woody plant species. Over the past several decades, in vitro micrografting has become a strategy to facilitate shoot recovery and acclimatization of in vitro-grown horticultural species. This review focuses on studies on horticultural crops over the past two decades that cover the establishment of in vitro micrografting, discusses factors affecting the success of in vitro micrografting, and provides commentary on the contribution of micrografting applications to the field of micropropagation. Considering the important roles of micrografting in the restoration of vigor and rooting competence, in promotion of shoot recovery following somatic embryogenesis and organogenesis, and in facilitation of shoot regrowth after cryopreservation, the potential use of this technique in facilitation of genetic engineering and safe conservation of horticultural species are specially highlighted.

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In-vitro grafting – Current applications and future prospects

Cited by 22 publications

References 50 publications

Grafting: a potential method to reveal the differential accumulation mechanism of secondary metabolites

Grafting: a potential method to reveal the differential accumulation mechanism of secondary metabolites

Shoot tip cryotherapy for plant pathogen eradication

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

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