In Vitro Genotoxicity of Wastewaters from the Town of Settat, Morocco

Asslouj, Jaouad El; Amahdar, Loubna; Glouib, Kamal; Kholtei, Sanae; Paaza, N. El Amrani; Verschaeve, Luc; Hilali, Abderraouf

doi:10.2478/10004-1254-60-2009-1926

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…The highly toxic effect of heavy metals on plants genotoxicity is because of its ability to inhibit cell division by inducing chromosomal aberrations . Genotoxicity such as drop in mitotic index and increase in the micronucleus have been reported in many previous studies . The mitotic index indicates the frequency of cell division.…”

Section: Introductionmentioning

confidence: 99%

Seed germination, root elongation, root‐tip mitosis, and micronucleus induction of five crop plants exposed to chromium in fluvo‐aquic soil

Hou

Liu

Xue

et al. 2014

Enviro Toxic and Chemistry

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The present study aimed to determine the toxic effects of chromium (Cr) on cabbage (Brassica oleracea), cucumber (Cucumis sativus), lettuce (Lactuca sativa), wheat (Triticum aestivum), and corn (Zea mays), and identify the sensitive plant species and appropriate bioassays for potential use in phytotoxicity assessment of Cr in soil. Results showed that seed germination might not be a sensitive assay for assessing Cr toxicity because at most of the Cr levels there were no toxic effects. Root elongation was more sensitive to Cr than seed germination. The lowest concentration of adverse effect (LOAEC) of lettuce was 20 mg Cr/kg(-1) soil, and that of the other 4 species was 50 mg Cr/kg(-1) soil. The mitotic index fluctuated with increasing Cr concentration, thus it was insufficient to assess toxicity of Cr in soil. However, micronucleus assay showed that 5 mg Cr/kg(-1) soil caused a significant increase in micronucleus frequency in cabbage, cucumber, and lettuce. For wheat and corn, however, the LOAEC was 20 and 50 mg/Cr/kg(-1) soil, respectively. Furthermore, the analysis of Cr accumulation showed that lettuce significantly accumulated Cr for all the tested concentrations. However, corn and wheat significantly accumulated Cr only with the highest tested dose. This may explain the higher inhibitory effects of Cr on root growth. It can be concluded that root elongation and micronucleus assay are good indicators to assess the phytotoxicity of Cr in soil. Lettuce is the most sensitive species for indicating the toxicity of Cr in soil.

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Section: Introductionmentioning

confidence: 99%

Seed germination, root elongation, root‐tip mitosis, and micronucleus induction of five crop plants exposed to chromium in fluvo‐aquic soil

Hou

Liu

Xue

et al. 2014

Enviro Toxic and Chemistry

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“…A similar study carried out by Amaral et al 43 applied SMART in Drosophila melanogaster to evaluate the genotoxicity of the Ca ı River, employing crude samples as well as 50% and 25% concentrations. El Asslouj et al 44 performed the CBMN assay to assess the genotoxicity of the Bounoumossa River, adding 50 mL, 100 mL, 200 mL and 400 mL of wastewater to the lymphocyte cultures. The authors recorded an increase in micronucleus frequency proportional to increasing doses.…”

Section: Discussionmentioning

confidence: 99%

Micronucleus study of the quality and mutagenicity of surface water from a semi-arid region

et al. 2011

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The present study evaluated the mutagenic potential of surface water from the Lucrecia dam. The Tradescantia-micronucleus (Trad-MCN) test and CBMN assay in human peripheral blood lymphocytes were applied, corresponding to an in vivo and in vitro system, respectively. Heavy metals and some physicochemical properties were also measured. Water samples were collected in November 2009 (dry season) and May 2010 (rainy season) at three different points. Results of both assays for raw water showed positive responses for the points analyzed when compared to the negative control. The CBMN assay showed that diluted water was still able to induce a significant increase in micronucleus frequency. For both assays, the highest mean MN was observed in the dry season. Chemical analyses detected an increase in heavy metal levels at the sampling points and in the different seasons. These findings indicate the presence of genotoxins, such as heavy metals, in the water, which may be affecting the entire ecosystem, as well as human health. More prolonged monitoring is recommended in order to better characterize this public water supply.

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Buccal micronucleus cytome assay of populations under chronic heavy metal and other metal exposure along the Santiago River, Mexico

Gómez‐Meda

Zúñiga‐González

Sánchez-Orozco

et al. 2017

Environ Monit Assess

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The Santiago River is one of the most contaminated rivers in Mexico, with heavy metal levels above the allowed limits. Scientific evidence indicates that chronic heavy metal exposure leads to cytogenotoxic effects. The aims of this study were to evaluate the genotoxic and cytotoxic effects of such exposure in buccal mucosa cells by micronucleus (MN) assay and to identify other nuclear abnormalities (NAs), such as nuclear buds (NBUDs), binucleated cells (BNs), pyknotic nuclei (PNs), karyorrhexis (KX), karyolysis (KL), and abnormally condensed chromatin (CC). Assays were performed on samples from four populations located alongside the Santiago River that are under chronic exposure to heavy metals and other metals (HMMs), and the results were compared with those of a population without exposure to HMMs. The exposed group showed increased frequencies of NAs (KX, CC, and KL), which are associated with cytotoxic damage, and NBUDs, which are associated with genotoxic damage. Increased frequencies of NBUDs and CC were observed in subjects from El Salto/Juanacatlán, Ocotlán, and Paso de Guadalupe, and an increase in KX frequency was observed in subjects from El Salto/Juanacatlán. Significant differences in KL frequency were observed in subjects from La Barca, El Salto/Juanacatlán, Paso de Guadalupe, and Ocotlán. Predictors for increased development of MNs and NBUDs were high concentrations of Al, Zn, and Cu. In conclusion, chronic exposure to HMMs, especially Al, Cu, and Zn, in the studied population could be related to increased frequencies of NAs, such as NBUDs, KX, CC, and KL, in the buccal mucosa cells.

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In Vitro Genotoxicity of Wastewaters from the Town of Settat, Morocco

Cited by 6 publications

References 18 publications

Seed germination, root elongation, root‐tip mitosis, and micronucleus induction of five crop plants exposed to chromium in fluvo‐aquic soil

Seed germination, root elongation, root‐tip mitosis, and micronucleus induction of five crop plants exposed to chromium in fluvo‐aquic soil

Micronucleus study of the quality and mutagenicity of surface water from a semi-arid region

Buccal micronucleus cytome assay of populations under chronic heavy metal and other metal exposure along the Santiago River, Mexico

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