In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage (‘comet’ assay) in relation to the induction of sister-chromatid exchanges and micronuclei

Villarini, Milena; Moretti, Massimo; Pasquini, Rossana; Scassellati-Sforzolini, Giuseppina; Fatigoni, Cristina; Marcarelli, Massimiliano; Monarca, Silvano; Rodrı́guez, Alberto Vicent

doi:10.1016/s0300-483x(98)00097-3

Cited by 109 publications

(50 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For this reason, these organisms are extremely sensitive to neurotoxic effects of pyrethroids when they reach surface water-courses (Bradbury andCoats 1989, Haya, 1989;Mittal et al, 1994). One of the pyrethroids that has found wide acceptability and is extremely used in agriculture and forestry because of its high activity against a broad spectrum of insect pests (Villarini et al, 1998) is deltamethrin ((S)α-cyano-3-phenoxybenzyl-(1R)-cis-3-(2.2-dibromovinyl)-2,2-dimethyl cyclopropane carboxylate). However, its effects on nervous, respiratory, and hematological systems in fishes have been reported (Ural andSağlam, 2005, Pimpão et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Biochemical biomarkers of exposure to deltamethrin in freshwater fish, Ancistrus multispinis

Assis

Nicareta

Salvo

et al. 2009

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Biochemical biomarkers of exposure to deltamethrin in freshwater fish, Ancistrus multispinis

Assis

Nicareta

Salvo

et al. 2009

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…The genotoxic and/or cytotoxic potential of some Type II pyrethroid insecticides such as cyfluthrin [Tisch et al, 2005], cypermethrin [Amer and Aboul-Ela, 1985;Bhunya and Pati, 1988;Amer et al, 1993;Chauhan et al, 1997Chauhan et al, , 1999Hadnagy et al, 1999;Institóris et al, 1999;Giri et al, 2003;Patel et al, 2006;Suman et al, 2006], deltamethrin [Agarwal et al, 1994;Surrallés et al, 1995;Gandhi et al, 1995;Villarini et al, 1998], fenvalerate [Pati and Bhunya, 1989;Puig et al, 1989;Surrallés et al, 1990;Caballo et al, 1992;Ghosh et al, 1992;Giri et al, 2002], and k-cyhalothrin [Campana et al, 1999;Ç elik et al, 2003, 2005a, 2005bNaravaneni and Jamil, 2005] has been shown in previous studies.…”

Section: Introductionmentioning

confidence: 99%

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

Kocaman

Topaktaş

2008

Environ and Mol Mutagen

View full text Add to dashboard Cite

a-Cypermethrin, a highly active pyrethroid insecticide, is effective against a wide range of insects encountered in agriculture and animal husbandry. The potential genotoxicity of a commercial formulation of a-cypermethrin (Fastac 100 EC, containing 10% a-cypermethrin as the active ingredient) on human peripheral lymphocytes was examined in vitro by sister chromatid exchange (SCE), chromosomal aberrations (CAs), and micronucleus (MN) tests. The human lymphocytes were treated with 5, 10, 15, and 20 lg/ml of a-cypermethrin for 24-and 48-hr. a-Cypermethrin induced SCEs and CAs significantly at all concentrations and treatment times and MN formation was significantly induced at 5 and 10 lg/ml of a-cypermethrin when compared with both the control and solvent control. Binuclear cells could not be detected sufficiently in the highest two concentration of a-cypermethrin (15 and 20 lg/ml) for both the 24-and 48-hr treatment times. a-Cypermethrin decreased the proliferation index (PI) at three high concentrations (10, 15, and 20 lg/ml) for both treatment periods as compared with the control groups. In addition, acypermethrin reduced both the mitotic index (MI) and nuclear division index (NDI) significantly at all concentrations for two treatment periods. The PI and MI were reduced by a-cypermethrin in a concentration-dependent manner during both treatment times. In general, a-cypermethrin showed higher cytotoxic and cytostatic effects than positive control (MMC) at the two highest concentrations for the 24-and 48-hr treatment periods. The present study is the first to report the genotoxic and cytotoxic effects of commercial formulation of a-cypermethrin in peripheral blood lymphocytes. Environ. Mol. Mutagen. 50:27-36, 2009. V V C 2008 Wiley-Liss, Inc.

show abstract

“…Primary DNA damage was evaluated in the in vitro culture of isolated human peripheral blood lymphocytes with the comet assay after a 1-h treatment period. To date, several studies have been performed to evaluate the genotoxic and/or cytotoxic potential of different commercial pesticide formulations by means of various test systems (Koller et al 2012;Moretti et al 2002;Ribas et al 1997;Santovito et al 2011;Undeger and Basaran 2005;Villarini et al 1998). As far as is known, there are no reports related to DNA damage induced by the commercial formulation CP.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity of commercial fungicide Cabrio Plus on human cell

Çayır

Çoşkun

2015

Cytotechnology

View full text Add to dashboard Cite

Cabrio Plus, a commercial fungicide, is used in agriculture as the control agent for a broad spectrum of diseases including black dot, early blight, late blight and powdery mildew. This study aimed to evaluate the genotoxicity of commercial formulation of Cabrio Plus which has been inadequately evaluated. The genotoxic potential of Cabrio Plus in isolated human peripheral blood lymphocytes was measured by means of an alkaline version of the comet assay (pH [ 13) and in whole blood by use of the in vitro micronucleus test. Cabrio Plus induced a statistically significant increase in DNA damage assessed with the in vitro micronucleus assay and the comet assay. Cabrio Plus also induced cytotoxicity in a dosedependent manner with the in vitro micronucleus assay. It can be concluded that a commercially available pesticide formulation, Cabrio Plus, has the ability to cause DNA damage and cytotoxicity.

show abstract

In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage (‘comet’ assay) in relation to the induction of sister-chromatid exchanges and micronuclei

Cited by 109 publications

References 41 publications

Biochemical biomarkers of exposure to deltamethrin in freshwater fish, Ancistrus multispinis

Biochemical biomarkers of exposure to deltamethrin in freshwater fish, Ancistrus multispinis

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

Genotoxicity of commercial fungicide Cabrio Plus on human cell

Contact Info

Product

Resources

About