In vitro fertilization plus preimplantation genetic diagnosis in patients with recurrent miscarriage: an analysis of chromosome abnormalities in human preimplantation embryos

Pellicer, A.; Rubio, Carmen; Vidal, Francesca; Mı́nguez, Yolanda; Giménez, Carles; Egozcue, J.; Remohı́, José; Simón, Carlos

doi:10.1016/s0015-0282(99)00143-0

Cited by 126 publications

(67 citation statements)

References 26 publications

(35 reference statements)

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“…The majority of embryos analyzed exhibited good morphology and embryo development prior to blastomere dissociation for FISH analysis. The high frequency of aneuploidy in human preimplantation embryos has been associated with increasing maternal age (7)(8)(9)(10)(11). Similarly, embryos that display poor development and/or morphology may exhibit more chromosomal abnormalities (7,8,11).…”

Section: Discussionmentioning

confidence: 99%

“…Most studies screening human preimplantation embryos for abnormalities only assess 1 or 2 blastomeres, which may not accurately reflect the chromosome complement of the remaining blastomeres because of significant incidences of chromosomal mosaicism (6)(7)(8)(9)(10)(11)(12)(13). Published studies generally concur on the cytogenetic classification of haploid, polyploid, normal and aneuploid human embryos.…”

Section: Introductionmentioning

confidence: 99%

“…Reported abnormality rates in human preimplantation embryos range from 19.3-81%, with 20-54% being mosaic and/or chaotic, 1.5-37.2% being aneuploid, 1.1-45.5% being polyploid and 0-7% being haploid. Chromosomal abnormality rates fluctuate and correlate with maternal age (7)(8)(9)(10)(11), recurrent pregnancy loss (10,12), failed IVF cycles (9,13), and poor embryo quality (7,8,11). In general, during preimplantation genetic diagnosis (PGD), single blastomeres removed from 8-cell human embryos are analyzed for chromosomal abnormalities that frequently occur in newborns (X, Y, 13,18,21) and in spontaneous abortions (X, Y, 13, 16, 18, 21 and 22) (2,3).…”

Section: Introductionmentioning

confidence: 99%

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Chromosomal instability in rhesus macaque preimplantation embryos

Dupont

Froenicke

Lyons

et al. 2009

Fertility and Sterility

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Objective-To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos. Design-Prospective rhesus monkey IVF study.Setting-Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center. Patients-Young Rhesus macaque females.Interventions-In vitro produced (IVP) entire rhesus macaque preimplantation embryos were cytogenetically assessed using a 5-color fluorescent in situ hybridization (FISH) assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X and Y, using human bacterial artificial chromosome (BAC) probes.Main Outcome Measure(s)-Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established.Result(s)-Fifty preimplantation embryos, displaying good morphology and normal development, from 11 young rhesus macaque females were analyzed. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid and 4 embryos (8%) triploid. Conclusion(s)-These data indicate that IVP rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chromosomal instability in rhesus macaque preimplantation embryos

Dupont

Froenicke

Lyons

et al. 2009

Fertility and Sterility

View full text Add to dashboard Cite

show abstract

“…58 The same is true for patients with a history of unexplained recurrent miscarriages. 59,60 Hypothetically, if we select chromosomally normal embryos for transfer, the miscarriage rate should be reduced. Some authors have published data suggesting that aneuploidy screening in these embryos significantly reduces the pregnancy loss rate.…”

Section: Preimplantation Genetic Diagnosis and Screeningmentioning

confidence: 99%

Advances and Controversies in Assisted Reproductive Technology

Vela

Luna

Sandler

et al. 2009

Mount Sinai J Medicine

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As the incidence and awareness of infertility have increased in the last decades, reproductive endocrinologists and scientists have striven to improve the therapeutic options being offered to patients. One of the most advanced and efficient technologies currently being offered is assisted reproductive technology. Among the various techniques that are comprised by assisted reproductive technology, in vitro fertilization is the most widely studied and used, being responsible for approximately 1% of all live births in the United States. As this technology has evolved, many controversies have arisen, and it is the purpose of this article to review what in the authors' opinion are the most current and controversial aspects of the whole process of in vitro fertilization and its related techniques. Mt Sinai J Med 76:506‐520, 2009. © 2009 Mount Sinai School of Medicine

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“…Although it is an invasive method, pre-implantation genetic diagnosis (PGD)-followed by fluorescence in situ hybridization (FISH)-may be performed to aneuploidy screening at 8-cell stage (15,18,19). While PGD has the advantage of selecting numerically normal embryos for some chromosomes, this technique is only indicated for patients reporting the following conditions: advanced maternal age, recurrent miscarriage, severe male factor, and genetic diseases.…”

Section: Introductionmentioning

confidence: 99%

The impact of pronuclear orientation to select chromosomally normal embryos

Borges

Rossi

Farah

et al. 2005

J Assist Reprod Genet

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Purpose : To find an effective method to select embryos from ICSI with better chromosomal status when preimplantation-genetic-diagnosis (PGD) is not applied. Methods : Several morpholological evaluations were done in same embryos. Embryos from ICSI were classified on day 1 according to pronuclear-nucleoli arrangement. On day 3, classification was done according to number, fragmentation, size and shape of cells. In some patients, embryos exhibiting good quality on day 3 (at least six regular blastomeres with cell fragmentation lower than 20%) were also submitted to PGD, irrespective to pronuclearnucleoli morphology. Results : Forty-two per cent of normally fertilized embryos showed pronuclear-nucleoli-goodmorphology; from those, 86% were also classified as good quality on day 3. Good-quality embryos submitted to PGD have shown lower chromosomal abnormality rates when also classified as pronuclear-nucleoli-good-morphology. Conclusions : Pronuclear-nucleoli morphology seems to be correlated with PGD results. This criterion may prove useful for pre-select embryos with normal chromosomal package when PGD is not applied.

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In vitro fertilization plus preimplantation genetic diagnosis in patients with recurrent miscarriage: an analysis of chromosome abnormalities in human preimplantation embryos

Cited by 126 publications

References 26 publications

Chromosomal instability in rhesus macaque preimplantation embryos

Chromosomal instability in rhesus macaque preimplantation embryos

Advances and Controversies in Assisted Reproductive Technology

The impact of pronuclear orientation to select chromosomally normal embryos

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