In vitro fertilization of bovine follicular oocytes and their development up to hatched blastocysts in vitro.

Kajihara, Y.; Goto, Kazufumi; Kosaka, S.; Nakanishi, Yoshihiko; Ogawa, Kohei

doi:10.1262/jrd1977.33.173

Cited by 52 publications

(20 citation statements)

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“…In contrast, Kajihara et al (1987) (Whittingham, 1971) and once with the maturation medium (25 mM-Hepes TCM 199 with Earle's salts; Gibco, Cat. 380-2340, NY, U.S.A.) supplemented with 5% neonatal calf serum (Gibco, Lot.…”

Section: Introductionmentioning

confidence: 99%

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Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes

Goto

Kajihara²,

Kosaka³

et al. 1988

Reproduction

259

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Section: Introductionmentioning

confidence: 99%

“…The method used was similar to that of Kajihara et al (1987). Briefly the following procedure was used.…”

Section: Introductionmentioning

confidence: 99%

Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes

Goto

Kajihara²,

Kosaka³

et al. 1988

Reproduction

259

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“…The IVM oocytes were subsequently fertilized in vitro (time 0 of IVF embryos). After IVF, the oocytes were cultured at 38.5 C in a 500-μl droplet of the maturation medium with cumulus cells (IVC medium) in an atmosphere of 3% CO2 in air [1]. Embryos at the zygote to hatched blastocyst stage 0-9 d after the beginning of IVF were used for the following experiments.…”

mentioning

confidence: 99%

“…VP embryos such as in vitro maturation/in vitro fertilization/in vitro culture (IVM/IVF/IVC) embryos [1,2] and nuclear transfer (NT) embryos [3,4] have been shown to lower developmental ability [5,6], lower tolerance to low temperatures and freezing [7,8] and lower pregnancy rates [3,9] compared to in vivo-collected (VIVO) embryos. Therefore, improvements of IVP systems and methods of evaluating produced IVP embryos in the livestock industry to produce calves might be reexamined based on the development of VIVO embryos.…”

mentioning

confidence: 99%

Transition of Cell Numbers in Bovine Preimplantation Embryos: In Vivo Collected and In Vitro Produced Embryos

Ushijima

Akiyama²,

Tajima

2008

Journal of Reproduction and Development

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Abstract. The total cell numbers (TCNs) of bovine embryos collected from superovulated donors (VIVO embryos) were counted 0-9 d after ovulation to quantify the developmental process. Using numerical analysis of embryo development, we also compared the developmental process of VIVO embryos, in vitro-fertilized (IVF) embryos and nuclear transfer (NT) embryos obtained from enucleated oocytes and blastomere nuclei. The TCNs of embryos were measured using the air-dry method. Cleavage divisions (CD) of the embryos were obtained using logarithmic transformation of the TCN. The TCN of the VIVO embryos increased significantly (P<0.001) with time. The relationship between the CD of the VIVO embryos at 0-9 d after ovulation and age in days was described by a linear equation with a high correlation (y=1.03x+0. 16, r=0.99), showing that CD occurs about once each day for all blastomeres. However, compared to the VIVO embryos, the TCN of the IVF embryos did not increase from 3-4 d nor after 7 d; the TCN of the NT embryos did not increase after 7 d (P>0.05). The results suggest a delay in development at these developmental stages. The slopes of regression lines of the IVF and NT embryos were significantly (P<0.001) smaller, indicating that quantification of the developmental process of VIVO embryos according to TCN and CD would be useful as criteria for numerical evaluation of the developmental process of bovine in vitro produced embryos. Key words: Bovine, Cleavage division, Preimplantation embryo, Total cell numbers (J. Reprod. Dev. 54: [239][240][241][242][243] 2008) VP embryos such as in vitro maturation/in vitro fertilization/in vitro culture (IVM/IVF/IVC) embryos [1, 2] and nuclear transfer (NT) embryos [3,4] have been shown to lower developmental ability [5,6], lower tolerance to low temperatures and freezing [7,8] and lower pregnancy rates [3,9] compared to in vivo-collected (VIVO) embryos. Therefore, improvements of IVP systems and methods of evaluating produced IVP embryos in the livestock industry to produce calves might be reexamined based on the development of VIVO embryos.Because the preimplantation embryo grows by self-replicating its blastomeres [10,11], total cell numbers (TCN) of embryos and cleavage divisions (CD) of embryos, as calculated from TCN, are commonly used to evaluate the developmental ability of IVC embryos [5,8,12]. A regression relationship between CD and embryo age in days also allows expression of the embryonic developmental process as segmental speed of blastomeres. Therefore, this numerical evaluation is useful for comparing the developmental ability and production methods of embryos [13][14][15]. However, bovine VIVO embryos used in TCN measurements are limited to those at a certain period in the late stage of culture [15], with no one yet having obtained measurements for all developmental stages of IVP embryos continuously during the 0-9 d period. Consequently, we consider that establishing an optimal timeline based on bovine VIVO embryos using the TCN and CD of embryos would be useful ...

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“…, cumulus cells (Kajihara et al, 1987), granulosa cells (Rexroad, 1989) or bovine oviductal epithelial cells (Gandolfi and Moor, 1987;Eyestone et al, 1987). All these feeder cells used in coculture were able to improve in vitro development of bovine zygotes compared to a medium devoid of cells.…”

mentioning

confidence: 99%

Beneficial effects of Vero cells for developing IVF bovine eggs in two different coculture systems

Menck¹,

Guyader-Joly²,

Peynot³

et al. 1997

Reprod. Nutr. Dev.

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In vitro fertilization of bovine follicular oocytes and their development up to hatched blastocysts in vitro.

Cited by 52 publications

References 0 publications

Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes

Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes

Transition of Cell Numbers in Bovine Preimplantation Embryos: In Vivo Collected and In Vitro Produced Embryos

Beneficial effects of Vero cells for developing IVF bovine eggs in two different coculture systems

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