Beneficial effects of Vero cells for developing IVF bovine eggs in two different coculture systems

Menck, M.C.; Guyader-Joly, C.; Peynot, Nathalie; Bourhis, Daniel Le; Lobo, R.B; Renard, J.P.; Heyman, Yvan

doi:10.1051/rnd:19970202

Cited by 40 publications

(24 citation statements)

References 24 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our developmental data are similar to those obtained by Menck et al [19] with Vero cells and B2, and those reported by Holm et al [27] for embryos developed in SOF. Although some data about culturing embryos in SOF with high BSA concentrations have been reported [37], no comparisons among SOF with FCS or high BSA concentrations, and co-culture with Vero cells have been described in terms of vitrification survival.…”

Section: Discussionsupporting

confidence: 93%

“…Also Vero cells have been employed together with B2 medium supplemented with fetal calf serum (FCS) for producing in vitro bovine embryos [19]. Simplification of in vitro culture conditions offers the advantage of reducing the number of problems due to changes in the composition of biological fluids and co-culture with somatic cells, and consequently, other media for producing bovine embryos in vitro have been developed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

et al. 2008

View full text Add to dashboard Cite

The aim of this study was to examine the effects of co-culture with Vero cells during the in vitro maturation (IVM) and three culture media, B2 + 5% fetal calf serum (FCS) on Vero cells, synthetic oviduct fluid (SOF) + 5% FCS, and SOF + 20 g L À1 bovine serum albumin (BSA), on the developmental competence of the embryos and their ability to survive vitrification/warming. We also tested the effect of morphological quality and the age of the embryo on its sensitivity to vitrification. The IVM system neither affects the embryo development up to Day 7 nor survival rates after vitrification. The culture of embryos in SOF + FCS and in Vero cells + B2 allowed obtaining more Day 6 and Day 7 blastocysts, and a higher % of Day 7 blastocysts vitrified than culture in SOF + BSA. Contrarily, on Day 8, more blastocysts were vitrified in SOF + BSA than in SOF + FCS. Blastocysts quality affected development after vitrification/warming, and Day 7 embryos showed higher survival rates than their Day 8 counterparts. Day 7 blastocysts produced in Vero cells or in SOF + BSA survived at higher rates than those produced in SOF + FCS at 24 and 48 h after warming. Embryo culture with BSA allows obtaining hatching rates after vitrification/warming higher than those obtained after coculture with Vero cells in B2 and FCS. Moreover, this system provides hatching rates from Day 8 blastocysts comparable to those obtained on Day 7 in Vero cells. Further studies, including embryo transfer to recipients, are needed to clarify factors affecting the freezability of in vitro produced bovine embryos. #

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

et al. 2008

View full text Add to dashboard Cite

show abstract

“…In our laboratory reconstituted embryos are cocultured in microdrops of B2 medium with vero cells at 39 °C under a controlled CO 2 atmosphere [14].…”

Section: In Vitro Culture Of Reconstituted Embryosmentioning

confidence: 99%

Nuclear transfer: a new tool for reproductive biotechnology in cattle

Heyman

2005

Reprod. Nutr. Dev.

View full text Add to dashboard Cite

-Recent evolutions of somatic cloning by nuclear transfer are reported, especially in the bovine species where potential applications are underway for biomedicine in association with transgenesis, or for agriculture by improving livestock. The overall efficiency of this biotechnology remains low in terms of viable offspring, but significant progress has been achieved on the different steps of the technique. However, the in vivo development of bovine blastocysts derived from somatic nuclear transfer is characterised by some important features that lead to the "cloning syndrome". Important losses occur during the peri-implantation period and further late foetal loss is observed in association with the Large Offspring Syndrome. About 60-70% of the cloned calves born survive normally to the adult stage and present an apparently normal physiology. Recent data already available on bovine somatic clones of both sexes indicate that they have a zootechnical performance similar to non cloned animals and they are able to reproduce normally without the pathologies associated to cloning thus confirming that the deviations observed in clones are of epigenetic origin and not transmitted to the progeny. biotechnology / reproduction / cattle / somatic cloning / nuclear transfer / efficiency

show abstract

“…In vitro development of the different groups of zygotes (delipidated, sham or controls) up to the blastocyst stage was achieved using the coeulture technique on Vero cells currently used in our laboratory for IVF embryos (23). Briefly, groups of 20 to 25 zygotes were placed in drops of 50 laL B2 medium supplemented with 10% FCS, seeded with Vero cells and overlayed with mineral oil.…”

Section: In Vitro Culturementioning

confidence: 99%

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Díez

Heyman

Bourhis³

et al. 2001

Theriogenology

Self Cite

View full text Add to dashboard Cite

To study the effect of partial removal of intracytoplasmatic lipids from bovine zygotes on their in vitro and in vivo survival, presumptive zygotes were delipidated by micromanipulation and cocultured with Veto cells in B2+10% FCS. Blastocyst rates of delipidated (n=960), sham (centrifuged but not delipidated, n=830) and control embryos (n=950) were 42.1, 42.3 and 39.9% respectively (P > 0.05). Day 7 blastocysts derived from delipidated zygotes had a mean of 123.9 + 45.6 nuclei compared to 137.5 + 32.9 for control blastocysts (P > 0.05). The full-term development of delipidated blastocysts after single transfer to recipients was similar to that of control IVF blastocysts (41.2% vs 45.4% respectively). To assess the effect of delipidation on the embryo tolerance to freczing/thawing, delipidated (n=73), control (n=67) and shmn (n=50) Day 7 blastocysts were frozen in 1.36 M glycerol + 0.25 M sucrose in PBS. After thawing, embryos were cocultured for 72 h with Vero cells in B2+10% FCS. Survival rates at 24 h were not significantly different between groups. However, in the delipidated group, the survival rate after 48 h in culture was significantly higher than in the control group (56.2 vs 39.8, P < 0.02), resulting in a higher hatching rate after 3 days in culture (45.2 vs 22.4, P < 0.02). Pregnancy rates for delipidated and control frozen/thawed embryos were respectively 10.5 and 22.2% (P > 0.05). Electron microscopic observations showed much fewer lipid droplets (and smaller) in delipated blastocysts than in controls. Taken together, our data show that delipidation of one cell stage bovine embryos is compatible with their normal development to term and has a beneficial effect on their tolerance to freezing and thawing at the blastocyst stage. This procedure, however, alters the developmental potential of such blastocysts, suggesting that maternally inherited lipid stores interfere with metabolic recovery after thawing.

show abstract

Beneficial effects of Vero cells for developing IVF bovine eggs in two different coculture systems

Cited by 40 publications

References 24 publications

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

Nuclear transfer: a new tool for reproductive biotechnology in cattle

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Contact Info

Product

Resources

About