In vitro fertilization of bovine oocytes matured in vivo and collected at laparoscopy

Effect of heparin on the expression of calmodulin-binding proteins in bull spermatozoa

“…Successful in-vitro fertilization has been achieved in cattle following capacitation treatment of spermatozoa with a hyperosmotic medium (Brackett et al, 1982;Lambert et al, 1986) or with the glycosaminoglycan heparin (Parrish et al, 1985(Parrish et al, , 1986a(Parrish et al, ,b, 1988. The molecular mechanisms are still not elucidated, but Ca2 + is recognized as one of the most important factors involved in the process of capacitation (Fraser, 1982(Fraser, , 1987.…”

Embryo Transfer andin vitroFertilization

“…Consequently, oocytes for research were obtained surgically from superovulated cattle in the 1970s and 1980s (Bederian et al, 1975;Brackett et al, 1980). Although laparoscopic procedures for oocyte retrieval via the paralumbar fossa were developed in the 1980s (Lambert et al, 1986), this approach, like surgical procedures, was expensive, ineffi cient, and risked the formation of adhesions with subsequent loss of fertility.…”

Aspectos técnicos e biológicos da obtençãode oócitos bovinos: revisão de literatura

“…Outro aspecto é que a colheita de embriões mostra-se inviável em algumas situações de infertilidade extra-ovariana, como hidrosalpinge bilateral (BOLS; VAN SOON;KRUIF et al, 1996b) e nas obstruções uterinas . Uma limitação adicional diz respeito à difícil aplicabilidade da TE em programas de biotecnologia, como a clonagem, que requer embriões em estádios iniciais de desenvolvimento (LAMBERT et al, 1986).…”