In vitro fecal fermentation characteristics of bamboo insoluble dietary fiber and its impacts on human gut microbiota

Ge, Qing; Li, Hang-qing; Zheng, Zhe-yuan; Yang, Kai; Xiao, Zhuqian; Xiao, Guo-ming; Mao, Jianwei

doi:10.1016/j.foodres.2022.111173

Cited by 32 publications

(15 citation statements)

References 34 publications

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“…The Chao1 index shows the microbial richness in samples. The Shannon and Simpson index show the community diversity [ 15 , 32 ]. After the in vitro fermentation, the Shannon index of SMF samples exhibited higher than that of SMCK group ( p < 0.05), accompanied by a slight increase in Chao1 and Simpson index.…”

Section: Resultsmentioning

confidence: 99%

“…Their production is related to the composition of intestinal microbiota, which can metabolize carbohydrates (e.g., dietary fiber, polysaccharides) and proteins in soymilk [ 33 , 34 ]. The previous studies revealed that the production of acetic acid, propionic acid was positively correlated with Bacteroidota , Proteobacteria , Lachnospiraceae [ 32 , 35 ]. It was consistent with the increase of acetic acid, propionic acid ( Figure 1 ) and the alteration of microbiota community in SMF ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the ratio of Firmicutes / Bacteroidota has an increase in inflammatory intestinal environment [ 8 ]. The ratio is also considered a biological indicator of obesity, and its reduction is inversely proportional to obesity [ 32 , 41 ]. In consistent with this study, the treatment with SMF resulted in 0.26-fold decrease, compared to the SMCK ( Figure 3 A,B).…”

Section: Discussionmentioning

confidence: 99%

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Effects of Bacillus subtilis BSNK-5-Fermented Soymilk on the Gut Microbiota by In Vitro Fecal Fermentation

Gao

Hou

et al. 2022

Foods

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The gut microbiota of soymilk intervention is beneficial to maintaining human health. Bacillus subtilis fermented soymilk has brought much interest, due to its richness of thrombolytic nattokinase and the strain of potential probiotic properties. In this study, soymilk was fermented by B. subtilis BSNK-5, and the BSNK-5-fermented soymilk (SMF) on the production of short chain fatty acids (SCFAs) and the regulation of fecal microbiota was initially evaluated by in vitro fecal fermentation. SMF supplementation obviously increased the levels of SCFAs from 32.23 mM to 49.10 mM, especially acetic acid, propionic acid, and isobutyric acid. Additionally, SMF changed the composition and microbial diversity of gut microbiota. After 24 h of anaerobic incubation in vitro, SMF decreased the Firmicutes/Bacteroidota ratio favoring weight loss, increased Lachnospiraceae_UCG-004 and the other beneficial bacteria producing SCFAs, as well as suppressing pathogenic Streptococcus genus. These results revealed the potential use of BSNK-5-fermented soymilk as a potential candidate to promote gut health.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of Bacillus subtilis BSNK-5-Fermented Soymilk on the Gut Microbiota by In Vitro Fecal Fermentation

Gao

Hou

et al. 2022

Foods

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“…Fermentation was studied in vitro according to the previously described method with slight modification ( 25 , 37 ). Briefly, after sterilized by UV irradiation for 12 h, 100 mg of samples (WIP, CMP, INU) were added into sterile fermentation containers, respectively.…”

Section: Methodsmentioning

confidence: 99%

Characteristics and properties of a polysaccharide isolated from Wolfiporia cocos as potential dietary supplement for IBS

Yang

Feng

et al. 2023

Front. Nutr.

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IntroductionAs low FODMAP (Fermentable oligosaccharides, disaccharides, monosaccharides and polyols) diet therapy is recommended for most of Irritable Bowel Syndrome (IBS) patients, the consequent insufficient of dietary fibers (DFs) intake exert an adverse impact on intestinal health. It is necessary to find suitable DFs for IBS patients.MethodsThis study extracted a water-insoluble polysaccharide from Wolfiporia cocos (WIP) by alkali-extraction and acid-precipitation method. Its molecular weight was detected by high performance gel permeation chromatography (HPGPC) analysis. The structure of WIP was analyzed by Fourier transform infrared (FT-IR) spectrum, Nuclear Magnetic Resonance (NMR) spectra and X-ray diffraction (XRD). The properties related to stability, digestion, viscosity, osmotic activity, adsorption and fermentation were investigated, aimed to explore the feasibility of WIP as a new DF supplement for patients with IBS. In addition, 16S rRNA sequencing analysis was conducted to explore its effects on IBS-related gut microbiota.Results and DiscussionThe results showed that WIP had a single homogeneous composition and the molecular weight was 8.1 × 103 Da. WIP was indicated as a kind of pyranose form with β anomeric configuration and the main chain of WIP was 1,3-β-glucan with amorphous structure. In addition to good thermal stability, WIP also has low bioavailability and can reach the colon mostly without being digested. Moreover, the low viscosity and osmotic activity, the high water- swelling and water/oil-holding capacity, fructose adsorption capacity and poor fermentation performance of WIP demonstrated that it is suitable for IBS patients. It is worth noting that WIP regulates IBS associated gut microbiota effectively, such as the abundance of Lachnospiraceae and Prevotella. These findings provide a theoretical basis for the development of WIP as a dietary supplement for IBS patients with low FODMAP diet therapy.GRAPHICAL ABSTRACT

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“…In terms of the role of DF in maintaining intestinal health, Ge [ 9 ] used an in vitro fermentation model to investigate the effect of insoluble dietary fiber from bamboo on human gut microbes and found an increase in the total short-chain fatty acid production, an increase in the relative abundance of Bacteroides , and a decrease in the ratio of Firmicutes to Bacteroides (F/B). Relative to single-component DF, one study found that soluble dextran produced greater changes in gut microbes than pectin polymers after in vitro fermentation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Fiber Compounds on Characteristic Human Flora and Metabolites Mediated by the Longevity Dietary Pattern Analyzed by In Vitro Fermentation

et al. 2022

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The purpose of this study was to investigate the effects of different dietary fiber compounds (DFCs) on characteristic human flora and their metabolites mediated by the longevity dietary pattern analyzed by in vitro fermentation. The results show that DFC1 (cereal fiber) increased the level of Lactobacillus (p < 0.05), DFC2 (fruit and vegetable and cereal fiber) promoted the growth of Lactobacillus and Bifidobacterium more significantly than DFC3 (fruit and vegetable fiber) (p < 0.01), and all three DFCs decreased the level of Escherichia coli (p < 0.05). The metabolomic analysis showed that there was variability in the metabolites and the metabolic pathways of different DFCs. The redundancy analysis revealed that the fiber content was positively correlated with Lactobacillus, Bifidobacterium, Bacteroides, acetic acid, butyric acid, propionic acid, lactic acid, and betaine, and negatively correlated with Escherichia coli, succinic acid, alanine, choline, aspartic acid, and α-glucose. Overall, this study found that different DFCs have different positive correlations on characteristic human flora and metabolites, and DFC2 is more favorable to the proliferation of the intestinal beneficial genera Lactobacillus and Bifidobacterium after in vitro fermentation, having a probiotic role in glucose, amino acid, and lipid metabolisms. This study may provide a theoretical reference for the search of optimal dietary fiber combination strategies mediated by longevity dietary pattern.

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In vitro fecal fermentation characteristics of bamboo insoluble dietary fiber and its impacts on human gut microbiota

Cited by 32 publications

References 34 publications

Effects of Bacillus subtilis BSNK-5-Fermented Soymilk on the Gut Microbiota by In Vitro Fecal Fermentation

Effects of Bacillus subtilis BSNK-5-Fermented Soymilk on the Gut Microbiota by In Vitro Fecal Fermentation

Characteristics and properties of a polysaccharide isolated from Wolfiporia cocos as potential dietary supplement for IBS

Effects of Dietary Fiber Compounds on Characteristic Human Flora and Metabolites Mediated by the Longevity Dietary Pattern Analyzed by In Vitro Fermentation

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