In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

Aghajanova, Lusine; Houshdaran, Sahar; Balayan, Shaina; Manvelyan, Evelina; Irwin, Juan C.; Huddleston, Heather G.; Giudice, Linda C.

doi:10.1007/s10815-018-1130-8

Cited by 82 publications

(74 citation statements)

References 73 publications

(90 reference statements)

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“…18 Another study published by Siwen Zhang demonstrated that menstrual blood-derived stromal cell along with platelet-rich plasma transplantation can improve the endometrial damage repairment and promote reproductive function in IUA rats. 20 Their results revealed that activated PRP can remarkably enhance the proliferation and migration of human endometrial cells, suggesting a potential application of activated PRP for endometrial regeneration in clinical settings. 19 Besides, a study conducted by Lusine Aghajanova evaluated the effect of activated platelet-rich plasma (PRP) on different types of Data are presented as Mean ± SEM; n = 5; *P < .05, **P < .01, and ***P < .001 vs the IUA group, ##P < .01 vs the Sham group human endometrial cells involved in the regeneration of endometrium.…”

Section: Discussionmentioning

confidence: 98%

Using 17β‐estradiol heparin‐poloxamer thermosensitive hydrogel to enhance the endometrial regeneration and functional recovery of intrauterine adhesions in a rat model

Zhang

Xiang

et al. 2019

FASEB j.

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Mechanical damage or infection to the endometrium can lead to the formation of adhesions in the uterine cavity, which may result in reduced reproductive outcome and/ or pregnancy complications. The prognosis of this disease is poor due to few effective treatments and the complex environment of endometrium. Heparin-Poloxamer Hydrogel (HP hydrogel) is a nontoxic and biodegradable biomaterial, which has been commonly used as a sustained-release delivery system. In this study, we applied a miniendometrial curette to scrape the endometrium of rats to mimic the process of curettage in patients. After the establishment of IUA model in rats, we injected the thermo-sensitive hydrogel(E 2 -HP hydrogel) into the injured uterine cavity and evaluated the therapeutic effect of E 2 -HP hydrogel on the recovery of IUA. Our results showed that E 2 -HP hydrogel can significantly facilitate the regeneration of injured endometrium along with inhibiting the cell apoptosis in IUA model. Furthermore, we revealed that E 2 -HP hydrogel on the recovery of IUA was closely associated with the upregulation of kisspeptin through activating the ERK1/2 and MAPKs p38 pathways. In conclusion, E 2 -HP hydrogel can effectively transfer E 2 into the injured endometrium and it can be considered as a promising therapeutic method for the women with intrauterine adhesions. K E Y W O R D S17β-estradiol, apoptosis, heparin-poloxamer hydrogel, intrauterine adhesions, kisspeptin, regeneration | 447 ZHANG et Al.

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Section: Discussionmentioning

confidence: 98%

Using 17β‐estradiol heparin‐poloxamer thermosensitive hydrogel to enhance the endometrial regeneration and functional recovery of intrauterine adhesions in a rat model

Zhang

Xiang

et al. 2019

FASEB j.

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“…The first in vitro study designed to elucidate the cellular mechanisms involved in PRP endometrial regeneration presented evidence that activated PRP increased cell migration and proliferation in different in vitro cell types and enhanced expression of matrix proteins [77]. However, the study also showed that platelet-poor plasma (PPP) and non-activated PRP preparations were also effective at promoting cell migration and proliferation and increased gene expression of matrix proteins.…”

Section: Platelet-rich Plasma and Endometrial Receptivitymentioning

confidence: 99%

Platelet-rich plasma or blood-derived products to improve endometrial receptivity?

Bös-Mikich

Ferreira

Oliveira³

et al. 2019

J Assist Reprod Genet

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The use of platelet-rich plasma (PRP) to improve endometrial receptivity is gaining increasing attention in assisted reproduction technologies. The authors report that autologous PRP intrauterine administration improves pregnancy and birth rates, particularly in cases of patients presenting poor endometrial growth. Different groups of scientists proposed a similar approach years ago using whole blood-derived products also to improve endometrial receptivity. The important role played by cytokines and growth factors during embryo implantation has been well-known for a long time. These signaling molecules are present and released by blood cells during physiological, normal endometrial growth and implantation. Similar blood mediators are released from platelet granules upon a blood vessel injury. Methods described for PRP preparation for intrauterine administration are not precise, and they seem to be similar to those used to prepare peripheral blood-derived products. Thus, it is possible that when preparing PRP from whole blood, the final plasma product used as BPRP^contains platelets in addition to the important cytokines and growth factors released by the peripheral blood mononuclear cells present in the whole blood. Precise knowledge of the identity, concentration, and effects of the individual blood factors, their origin, whether platelets or blood mononuclear cells, will greatly contribute to improve and to make results obtained in fertility treatments more repeatable.

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“…Given that the endometrium contains glands, epithelium and supportive stroma, the regenerative potency of endometrium comes from both epithelial and stromal stem/progenitor cells. 3,5 A number of studies indicated the existence of a population of endometrial mesenchymal stem/stromal cells (eMSC) in human endometrium after cultures from menstrual blood (MB). 1,3,6 These studies showed that the isolated eMSC were multipotent and their properties and phenotype were similar to bone marrow and adipose tissue MSC.…”

Section: Introductionmentioning

confidence: 99%

Receptivity markers in endometrial mesenchymal stem cells of recurrent implantation failure and non‐recurrent implantation failure women: A pilot study

Esmaeilzadeh

Mohammadi

Mahdinejad

et al. 2020

J of Obstet and Gynaecol

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Aim Endometrial mesenchymal stem cells (eMSC) have a vital role in regeneration of endometrium during menstrual cycles. Since it has been suggested that (eMSC) likely play a role in uterine receptivity and establishment of pregnancy, we aimed to evaluate the expression levels of five most known receptivity markers‐Integrin (ITG) β1, Rac1, HoxA11, ITGβ3 and Noggin‐in eMSC of recurrent implantation failure (RIF) and non‐RIF women. Methods Human eMSC were isolated from menstrual blood (MB) of RIF and non‐RIF women. The isolated eMSC characterized based on their morphological and behavioral characteristics, expression of MSC‐specific surface CD markers and their capacity of differentiation into osteocytes and adipocytes. The expression levels of the five mentioned receptivity markers were analyzed with real time reverse transcription polymerase chain reaction. Results Our findings revealed that RIF and non‐RIF eMSC expressed all tested genes at different levels. ITGb1 expression in RIF eMSC was lower than its expression in non‐RIF cells. On the other hand, all the other markers were expressed at higher levels in RIF eMSC than in non‐RIF cells although only HOXA11 and ITG β3 showed statistically significant (P < 0.05) higher expression levels. Conclusion This pilot study on determination of the expression levels of uterine receptivity markers in eMSC interestingly indicated that RIF and non‐RIF eMSC were different regarding the expression of these markers. Future studies using these findings can brighten up more the role of eMSC in the endometrium receptivity and establishment of pregnancy.

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In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

Cited by 82 publications

References 73 publications

Using 17β‐estradiol heparin‐poloxamer thermosensitive hydrogel to enhance the endometrial regeneration and functional recovery of intrauterine adhesions in a rat model

Using 17β‐estradiol heparin‐poloxamer thermosensitive hydrogel to enhance the endometrial regeneration and functional recovery of intrauterine adhesions in a rat model

Platelet-rich plasma or blood-derived products to improve endometrial receptivity?

Receptivity markers in endometrial mesenchymal stem cells of recurrent implantation failure and non‐recurrent implantation failure women: A pilot study

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