In vitro evaluation of the effects of methanolic plant extracts on the embryonation rate of Ascaridia galli eggs

Poulopoulou, I.; Horgan, Mark James; Siewert, Bianka; Siller, Michaela; Palmieri, L.; Martinidou, Eftychia; Martens, Stefan; Fusani, Pietro; Temml, Veronika; Stuppner, Hermann; Gauly, Matthias

doi:10.1007/s11259-022-09958-9

Cited by 5 publications

(6 citation statements)

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“…A. absinthium extract administrated orally to cats at a dose between 300–600 mg/kg bw showed a good anthelmintic activity [ 59 ]. Wormwood methanolic extracts demonstrated a strong in vitro inhibitory effect on the embryonation rate of A. galli eggs [ 60 ]. Several reports highlighted the anthelmintic activity of A. absinthium against A. suum and A. galli [ 61 – 63 ].…”

Section: Discussionmentioning

confidence: 99%

In vivo assessment of the antiparasitic effects of Allium sativum L. and Artemisia absinthium L. against gastrointestinal parasites in swine from low-input farms

Băieş,

Cotuţiu,

Spînu

et al. 2024

BMC Vet Res

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Background Ethno-veterinary practices could be used as a sustainable developmental tool by integrating traditional phytotherapy and husbandry. Phytotherapeutics are available and used worldwide. However, evidence of their antiparasitic efficacy is currently very limited. Parasitic diseases have a considerable effect on pig production, causing economic losses due to high morbidity and mortality. In this respect, especially smallholders and organic producers face severe challenges. Parasites, as disease causing agents, often outcompete other pathogens in such extensive production systems. A total of 720 faecal samples were collected in two farms from three age categories, i.e. weaners, fatteners, and sows. Flotation (Willis and McMaster method), modified Ziehl–Neelsen stained faecal smear, centrifugal sedimentation, modified Blagg technique, and faecal cultures were used to identify parasites and quantify the parasitic load. Results The examination confirmed the presence of infections with Eimeria spp., Cryptosporidium spp., Balantioides coli (syn. Balantidium coli), Ascaris suum, Oesophagostomum spp., Strongyloides ransomi, and Trichuris suis, distributed based on age category. A dose of 180 mg/kg bw/day of Allium sativum L. and 90 mg/kg bw/day of Artemisia absinthium L. powders, administered for 10 consecutive days, revealed a strong, taxonomy-based antiprotozoal and anthelmintic activity. Conclusions The results highlighted the therapeutic potential of both A. sativum and A. absinthium against gastrointestinal parasites in pigs. Their therapeutic effectiveness may be attributed to the content in polyphenols, tocopherols, flavonoids, sterols, sesquiterpene lactones, and sulfoxide. Further research is required to establish the minimal effective dose of both plants against digestive parasites in pigs.

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Section: Discussionmentioning

confidence: 99%

In vivo assessment of the antiparasitic effects of Allium sativum L. and Artemisia absinthium L. against gastrointestinal parasites in swine from low-input farms

Băieş,

Cotuţiu,

Spînu

et al. 2024

BMC Vet Res

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“…Studies on ethnoveterinary plants and the exploration of their antiparasitic effects most probably lead to the identification of promising active compounds not only for veterinary medicine but also for human health (Valente et al, 2021). C. alpina is a tall alpine herb that accumulates phytochemicals with demonstrated antiparasitic activity (Poulopoulou et al, 2022), while recent studies have also noted its strong antioxidant response and its potential as a treatment for metabolism-related diseases (Zheleva-Dimitrova et al, 2023). These characteristics render the species an attractive candidate for integration into the pharmaceutical and agricultural industries, warranting further research.…”

Section: Discussionmentioning

confidence: 99%

“…Until recently, its utilization was limited to such local culinary applications, food preparations, and salads. However, it has been discovered that leaf extracts from C. alpina exhibit potent antiparasitic activity against animal nematodes (Poulopoulou et al, 2022) which offers new application and use of the plant.…”

Section: Introductionmentioning

confidence: 99%

“…In a recent study, eggs of Ascaridia galli, an intestinal parasite that infects poultry and is responsible for 60-84% of annual production losses (Zaman et al, 2020), were treated in vitro with methanolic extracts of ten different known AH plants found in the Italian Alps. Among them, C. alpina extract showed the lowest rates of embryonic development and was therefore selected for further studies (Poulopoulou et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the chemical and genetic variability among accessions of Cicerbita alpina (L.) Wallr., an alpine plant with anthelmintic properties

Martinidou,

Palmieri,

Sordo

et al. 2023

Front. Plant Sci.

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Cicerbita alpina (L.) Wallr, is a perennial alpine plant and a member of the Asteraceae family, typically found at altitudes above 1000 meters in the Italian Alps. Although previously utilized primarily as a local delicacy, recent studies have revealed strong antiparasitic activity through in vitro experiments. In Europe, numerous chemical drugs employed to combat nematodes — helminths that infest the digestive tract of livestock — are banned due to their environmental harm or show only reduced efficiency because of the development of resistance. Consequently, there is a growing demand for new alternative anthelmintic treatments in agricultural practices. Specialized metabolites found in the extracts of C. alpina could offer a sustainable and biological alternative to chemical drugs, specifically for nematode control. For this purpose, a unique germplasm collection originating from eight distinct natural populations in the Italian Alps was analyzed for its chemical diversity using state-of-the-art targeted LC-MS/MS spectrometry, including quantification based on multiple reaction monitoring. The predominant metabolites identified from the species were the caffeic acid derivatives chicoric acid, chlorogenic acid, and 3. 5-dicaffeoylquinic acid, the sesquiterpene lactone derivative 8-O-acetyl-15-ß-D-glucopyranosyl lactucin and the flavone glycosides, apigenin-7-O-glucoside and luteolin-7-O-glucoside, alongside their precursors apigenin and luteolin, respectively. Additionally, the genetic diversity of eighty individual plants within the germplasm collection was evaluated using ten DNA molecular markers (Simple Sequence Repeats), successfully transferred from two closely related species (Cichorium intybus and Tanacetum parthenium). This investigation unveiled a significant range of genetic diversity within the examined populations, resulting in the establishment of three distinct genetic groups. The findings were further correlated with the original ecological environment and local climate conditions spanning a biennial period, indicating substantial variations among the different accessions and the intricate interplay between genetic background and environmental factors. These results could serve as a basis for future domestication of the species through plant breeding programs ensuring product quality, but also facilitating the cultivation of C. alpina in more diverse geographic regions.

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“…One widely studied heterocycle, nocodazole 5 , gave crucial information into the role of CBSIs in microtubule dynamics at the cell biology level . Established benzimidazoles with antimitotic mechanisms against human and veterinary parasites support their wider safe use as medications. , A recent prodrug lisavanbulin with a benzimidazole moiety, reached phase I/IIa trials for advanced solid tumors . Interestingly, the synthetic indole 2 demonstrated potent tumor growth inhibition in a taxane resistant model, suggesting it might circumvent certain types of MDR that taxanes cannot evade .…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel β-Tubulin Inhibitors Using a Combined In Silico/In Vitro Approach

Horgan,

Zell,

Siewert

et al. 2023

J. Chem. Inf. Model.

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Due to their potential as leads for various therapeutic applications, including as antimitotic and antiparasitic agents, the development of tubulin inhibitors offers promise for drug discovery. In this study, an in silico pharmacophore-based virtual screening approach targeting the colchicine binding site of β-tubulin was employed. Several structure- and ligand-based models for known tubulin inhibitors were generated. Compound databases were virtually screened against the models, and prioritized hits from the SPECS compound library were tested in an in vitro tubulin polymerization inhibition assay for their experimental validation. Out of the 41 SPECS compounds tested, 11 were active tubulin polymerization inhibitors, leading to a prospective true positive hit rate of 26.8%. Two novel inhibitors displayed IC50 values in the range of colchicine. The most potent of which was a novel acetamide-bridged benzodiazepine/benzimidazole derivative with an IC50 = 2.9 μM. The screening workflow led to the identification of diverse inhibitors active at the tubulin colchicine binding site. Thus, the pharmacophore models show promise as valuable tools for the discovery of compounds and as potential leads for the development of cancer therapeutic agents.

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In vitro evaluation of the effects of methanolic plant extracts on the embryonation rate of Ascaridia galli eggs

Cited by 5 publications

References 47 publications

In vivo assessment of the antiparasitic effects of Allium sativum L. and Artemisia absinthium L. against gastrointestinal parasites in swine from low-input farms

In vivo assessment of the antiparasitic effects of Allium sativum L. and Artemisia absinthium L. against gastrointestinal parasites in swine from low-input farms

Assessment of the chemical and genetic variability among accessions of Cicerbita alpina (L.) Wallr., an alpine plant with anthelmintic properties

Identification of Novel β-Tubulin Inhibitors Using a Combined In Silico/In Vitro Approach

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