In vitro evaluation of the oxidative stress and genotoxic potentials of anthracene on mycorrhizal chicory roots

Propiconazole Toxicity on the Non-Target Organism, the Arbuscular Mycorrhizal Fungus, Glomus irregulare

“…The disruption in polyunsaturated FA levels may therefore be related to the direct reaction of oxygen-free radicals with unsaturated lipids. These observations are in accordance with previous studies, which reported that abiotic stresses such as pollution, drought, salinity and heat induced an oxidative stress leading to MDA production by plant cells (Sinha et al, 2005;Bidar et al, 2007;He et al, 2007;Debiane et al, 2008Debiane et al, , 2009Yamauchi et al, 2008). In fact, the oxidative stress arising from abiotic stress exposure could generate ROS (Apel and Hirt, 2004), which can interact with polyunsaturated FA to generate aldehydes of which MDA is the main one (Esterbauer et al, 1991).…”

“…Besides fungicides, many studies reported negative effects on Glomus sp. development of various pollutants (i.e., polycyclic aromatic hydrocarbons and heavy metal) and other abiotic stresses (i.e., salinity) (Schützendübel and Polle, 2002;Verdin et al, 2006;He et al, 2007;Hildebrandt et al, 2007;Debiane et al, 2008Debiane et al, , 2009. In order to explain propiconazole toxicity on AMF development, changes in the lipids, especially FA, PL, PLFA and the lipid peroxidation biomarker MDA, were investigated in Glomus irregulare grown under propiconazole treatment.…”

“…After centrifugation (3 min/10000g), supernatants were divided in 250 μL-aliquots and supplemented with (2,6 di-tert-butyl-4-methylphenol at 2.5 g.L -1 ethanol) to determine MDA, peroxidase and proteins. A high performance liquid chromatography MDA assay was used to evaluate the MDA production as described by Debiane et al (2008). Tetraethoxypropane was used as the standard, and thiobarbituric acid (TBA) as the reagent.…”

“…The in vitro cultures allow non-destructive observations of AMF (Fortin et al, 2002), they are standardized and reproducible method enabling comparisons of various molecules impact on AMF (Debiane et al, 2008(Debiane et al, , 2009Hillis et al, 2008;Zocco et al, 2008;Campagnac et al, 2010). In addition, thanks to these monoxenic cultures, big amounts of biological material free of contaminant microorganisms can be obtained.…”

In vitrogermination and post-seminal development ofRuta graveolensL. under the influence of phenanthrene and benzo[a]pyrene

“…Ensaios sob condições controladas, como ocorre sob condições in vitro, demonstram que as respostas vegetais podem ser exclusivas da espécie em estudo, anulando os efeitos das variações sazonais e favorecendo a produtividade de plantas e substâncias de interesse (REYNOSO-CUEVAS et al, 2008;SINGH;CHATURVEDI, 2012). Além de visar respostas morfogênicas específicas, os sistemas in vitro também são indicados nos estudos relacionados à avaliação da capacidade de as células vegetais tolerar, detoxificar e armazenar/imobilizar substâncias orgânicas contaminantes, sendo possível estudar os fitormônios ativos sob tais condições, viabilizando a realização de testes de toxicidade com contaminantes orgânicos, em concentrações controladas (DEBIANE et al, 2008). Dentre os estudos de desenvolvimento vegetal in vitro, a germinação tem se mostrado uma estratégia importante visto que a própria composição do meio pode influenciar no início do processo e no estabelecimento in vitro de plantas (LENCINA et al, 2014), alterando parâmetros morfológicos e fisiológicos (VICTÓRIO; LAGE, 2009).…”