In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells

Semeghini, Mayara Sgarbi; Fernandes, Roger Rodrigo; Chimello, Daniela Thomazatti; Oliveira, Fabíola Singaretti; Bombonato-Prado, Karina Fittipaldi

doi:10.1590/s0103-64402012000400004

Cited by 13 publications

(12 citation statements)

References 25 publications

(30 reference statements)

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“…Several studies have shown that the use of resins as restorative materials is associated with irritation and necrosis of the pulp tissue. In addition, undifferentiated dental pulp cells might differentiate into odontoblasts to replace the cells that are destroyed by caries or dental restorative procedures [28–30]. …”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity of resin composites containing bioactive glass fillers

et al. 2015

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Objective To determine the in vitro cytotoxicity of dental composites containing bioactive glass fillers. Methods Dental composites (50:50 Bis-GMA/TEGDMA resin: 72.5wt% filler, 67.5%Sr-glass and 5% OX50) containing different concentrations (0, 5, 10 and 15 wt %) of two sol-gel bioactive glasses, BAG65 (65 mole% SiO2, 31 mole% CaO, 4 mole% P2O5) and BAG62 (3 mole% F added) were evaluated for cytotoxicity using Alamar Blue assay. First, composite extracts were obtained from 7 day incubations of composite in cell culture medium at 37° C. Undifferentiated pulp cells (OD-21) were exposed to dilutions of the original extracts for 3, 5, and 7 days. Then freshly cured composite disks were incubated with OD-21 cells (n=5) for 2 days. Subsequently, fresh composite disks were incubated in culture medium at 37°C for 7 days, and then the extracted disks were incubated with OD-21 cells for 2 days. Finally, fresh composites disks were light cured for 3, 5, and 20 seconds and incubated with OD-21 cells (n=5) for 1, 3, 5, and 7 days. To verify that the three different curing modes produced different levels of degree of conversion (DC), the DC of each composite was determined by FTIR. Groups (n=5) were compared with ANOVA/Tukey’s (α≤0.05). Results Extracts from all composites significantly reduced cell viability until a dilution of 1:8 or lower, where the extract became equal to the control. All freshly-cured composites showed significantly reduced cell viability at two days. However, no reduction in cell viability was observed for any composite that had been previously soaked in media before exposure to the cells. Composites with reduced DC (3 s vs. 20 s cure), as verified by FTIR, showed significantly reduced cell viability. Significance The results show that the composites, independent of composition, had equivalent potency in terms of reducing the viability of the cells in culture. Soaking the composites for 7 days before exposing them to the cells suggested that the “toxic” components had been extracted and the materials were no longer cytotoxic. The results demonstrate that the cytotoxicity of composites with and without BAG must predominantly be attributed to the release of residual monomers, and not to the presence of the BAG.

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Section: Methodsmentioning

confidence: 99%

Cytotoxicity of resin composites containing bioactive glass fillers

et al. 2015

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“…The suggested duration was selected in accordance with the other authors who examined the odontogenic potential of mouse undifferentiated pulp cells in vitro after 3, 7, 10 and 14 days from culture. (22) .…”

Section: Discussionmentioning

confidence: 99%

“…DMP-1 is also essential for the late dentinogenesis development (11) . ALP gene expression using RT-PCR test was selected in the present study as it is reported that ALP stimulates dentin matrix formation, mineralization It is also observed a correlation between the high levels of ALP and an increase in mineralized globules through Alizarin red stain (22) .…”

Section: To Confirm Odontoblastic Differentiation Frommentioning

confidence: 99%

In Vitro differentiation potential of Isolated dental pulp stem cells

Moussa

Hafez

Sabry

2018

Egyptian Dental Journal

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Objective: The aim of the present study is to isolate and characterize human dental pulp stem cells (DPSCs).Surface markers expression utilizing surface markers for flow cytometry. The odontogenic differentiation of DPSCs was assessed. Material and methods: dental pulp tissuses were collected from impacted third molars. samples were treated with collagenase before centrifugation to allow release of the cells. Cells were cultured in complete culture medium for 12-14 days. After reaching confluence, the isolated cells were characterized by flow cytometry using CD105 and CD45. The cells were induced for odontogenic differentiation by placing the cells in odontogenic induction media for 14 days. Odontogenic differentiation was evaluated by Alizarin Red stain and by the expressions of dentine sialo phospho protein (DSPP), dentin matrix protein-1 (DMP-1) and alkaline phosphatase (ALP) activity, which were assessed by RT-PCR. Results: The results showed that successful isolation of stem cells from human dental pulp was achieved. Cells were successfully sub-cultured and expanded up to the third passage. A flow cytometric analysis was done after stem cells isolation. Isolated DPSCs were identified by positive expression of CD105 and negative expression of hematopoietic marker C. The results showed that differentiatiationed odontoblastic like cells from DPSCs induced by odontogenic medium were stained by Alizarin Red at days 7 and 14. RT-PCR results indicated that all cultured cells efficiently differentiate into dentin forming cells and expressed specific DSPP, DMP1 and ALP genes at days 7 and 14. Conclusion: DPSCs can be a possible source of stable differentiated odontoblastic cells. Their ability of inducing hard tissue formation offer an alternative method to save teeth with compromised structural integrity.

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“…5 Undifferentiated pulp stem cells in the adult have the capacity to proliferate and differentiate into odontoblastic cells responsible for dentine formation and maintenance of its integrity. Former investigations have shown that pulp cells from a murine cell line , when chemically induced during cell culture, may present similar characteristics to odontoblast-like murine cells (MDPC-23), like higher ALP activity and production of mineralized nodules, 6 as well as expression of DMP1, one of the dentine noncollagenous extracellular matrix proteins that has been implicated in regulation of mineralization. 7 Other studies have shown that even some materials can influence and stimulate the odontogenic differentiation of DPSCs, like for example MTA, which is known to up-regulate genes such as Bmp-6, Bmp-2, and Nox-4, associated to the formation of bone, cartilage and connective tissues.…”

Section: Introductionmentioning

confidence: 99%