In vitro evaluation of the antimicrobial activity of endodontic sealers

Abstract

The purpose of this study is to evaluate the antimicrobial activity of the endodontic sealers: N-Rickert, Sealapex, AH Plus, Mineral Trioxide Aggregate (MTA) and portland cement. The Agar diffusion method was used in plates previously inoculated with the following microorganisms: C. albicans, S. aureus, E. faecalis, E. coli. The diameters of microbial inhibition zones were measured after 24 hours of incubation in kiln at 37 degrees C. According to the methodology used, it was possible to conclude that only the sealers AH Plus and N-Rickert presented antimicrobial activity against C. albicans, S. aureus, and E. coli; no antimicrobial activity in MTA, Sealapex and portland cement was observed. N-Rickert presented the largest inhibition zones varying from 8 to 18 mm, and the microorganism E. faecalis was resistant against all sealers tested.

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

“…However, in this study all materials were ineffective against E. faecalis, as similar to those observed in the literature related to Estrela et al 4 , Ribeiro et al 13 , Zarrabi et al 14 and Miyagak et al 24 , in constrast to the results presented in Sipert et al 11 and Tanumaru Filho et al 12 investigations.…”

Sealing ability of mineral trioxide aggregate, glass ionomer cement and composite resin when repairing large furcal perforations

“…Several studies have shown that MTA does not have any significant growth inhibiting effect on a number of oral bacteria. 36,37 In contrast to these findings, Eldeniz et al,38 evaluating the antibacterial activity of leachable components from selected rootend filling materials, found that set samples of ProRoot MTA cement completely inhibited Pseudomonas aeruginosa and delayed or limited growth of Enter. faecalis.…”

Antimicrobial effects of several calcium silicate-based root-end filling materials

“…The dissociation into calcium and hydroxide ions results in an increase in the pH and calcium ion release 22) . The antimicrobial effects of MTA have been widely assessed, but contradictory findings have been reported 3,18,[23][24][25][26] . An in vitro study showed that MTA exerts antibacterial effects against some facultative bacteria and but not on any species of absolute anaerobes 3) .…”

“…Some studies have shown that white MTA 25) and grey MTA 23,24) exert antifungal effects. However, others have shown that grey MTA has limited or no antifungal effects 19,26) . Torabinejad et al 27) reported that the pH of MTA is 10.5 at the time of mixing but 12.9 after 3 h. According to McHugh et al 28) , E. faecalis is unable to survive at pH values of 11.5 or greater.…”