In Vitro Evaluation of Probiotic Potential of Selected Lactic Acid Bacteria Strains

<i>In Vitro</i> Characterization of Cell Surface Properties of 14 Vaginal <i>Lactobacillus</i> Strains as Potential Probiotics

“…Biofilm formation varied in a large range among the tested vaginal Lactobacillus stains in this study. Similar results were previously found in vaginal Lactobacillus strains [2] [36] [37] and Lactobacillus strains of other origins [16], but most vaginal Lactobacillus strains were found to be weak biofilm producers by Malik et al [35]. On the other hand, a study by Klimko et al suggested the absence of correlation between high hydrophobicity and intense biofilm formation [16], but analysis of data from the 14 Lactobacillus strains in the current study suggested that biofilm formation was highly correlated with Lewis acidity, and moderately correlated with CSH, Lewis basicity and autoaggregation (Table 2).…”

“…Human-origin Lactobacillus is a preferable source of probiotic bacteria, and Lactobacillus isolates from human oral cavity [8] [14], breast milk [15] [16], stomach [17], feces or intestinal tract [18] [19] [20] have been studied on their probiotic characteristics. Lactobacillus plays an important role in maintaining the health of human vagina [4] [6].…”

Effect of Lactobacillus plantarum Biofilms on the Adhesion of Escherichia coli to Urinary Tract Devices

“…Other research groups evaluated the biofilm-forming capacity of L. plantarum and showed that different strains of L. plantarum were able to grow as a biofilm in MRS broth on abiotic surfaces, such as polystyrene and glass [29,[56][57][58][59]. Klimko et al [60] investigated the ability of LAB to form biofilms on a hydrophobic carrier, and L. plantarum was revealed to be the most prolific biofilm-forming bacterium. Several factors, such as the temperature, availability of nutrients, pH, contact time with the surface, growth stage, and surface hydrophobicity, can affect biofilm development [61].…”

“…The ability of microorganisms to adhere to certain substrata depends on van der Waals attraction forces, gravitational forces, steric interactions, protein adhesion, and electrostatic interactions, but one of the more important factors is the hydrophobicity of the cells [3,42,63]. The hydrophobicity of the cell surface depends on the surface components of the bacterial cells, such as proteins and polysaccharides, and the adhesion phenomenon is influenced by the contact between the cell wall and the surface of attachment [60,67]. Previous studies reported the hydrophilic character of L. plantarum [56,67], this being in accordance with the hydrophilicity level determined in the present work.…”

Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components

“…Similarly to EnoA1 and to other adhesins expressed on cell surface of different commensal or pathogen bacteria, L. plantarum PDHB is also involved in biofilm development [82]. The identification of lactobacilli adhesins involved in biofilm development, such as EnoA1 and PDHB, is of interest for the possible impact that the biofilm itself may have on persistence of the microorganism in the colon [86]. Furthermore, few studies showed that factors secreted from lactobacilli biofilms possess immunomodulatory properties [87,88].…”